Kribbella sp. VKM Ac-2527

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Kribbellaceae; Kribbella; unclassified Kribbella

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8831 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6JLY8|A0A4R6JLY8_9ACTN Glyoxylase-like metal-dependent hydrolase (Beta-lactamase superfamily II) OS=Kribbella sp. VKM Ac-2527 OX=2512215 GN=EV643_12216 PE=4 SV=1
MM1 pKa = 7.38TSIALLVGTTACSNDD16 pKa = 3.46NPPEE20 pKa = 4.04EE21 pKa = 4.67TPSSEE26 pKa = 4.0QTPAAVDD33 pKa = 3.87TPSSEE38 pKa = 4.14VSTVTPTEE46 pKa = 4.12VISRR50 pKa = 11.84LSGAGYY56 pKa = 8.31TCGRR60 pKa = 11.84DD61 pKa = 2.95GDD63 pKa = 4.27YY64 pKa = 11.14AICTTGAVAVWVLTGNHH81 pKa = 5.24TRR83 pKa = 11.84PPVVSLHH90 pKa = 6.37SEE92 pKa = 4.41GSADD96 pKa = 3.29VAAAAIGKK104 pKa = 7.74VLPQALEE111 pKa = 3.92LAQVSPAQPMVDD123 pKa = 3.03WFGEE127 pKa = 4.31QADD130 pKa = 3.94EE131 pKa = 4.22TAAQTTEE138 pKa = 4.3GDD140 pKa = 3.52WQIDD144 pKa = 3.15WSVEE148 pKa = 3.87VDD150 pKa = 3.52SEE152 pKa = 4.51EE153 pKa = 4.49PGAHH157 pKa = 6.78LSLMDD162 pKa = 4.24KK163 pKa = 10.92LCTANCQAEE172 pKa = 4.12

Molecular weight: 17.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R6KRN1|A0A4R6KRN1_9ACTN Uncharacterized protein OS=Kribbella sp. VKM Ac-2527 OX=2512215 GN=EV643_101232 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 5.34KK15 pKa = 10.47KK16 pKa = 8.84HH17 pKa = 5.5GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.64GRR40 pKa = 11.84QRR42 pKa = 11.84LAAA45 pKa = 4.56

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8831

0

8831

2844625

25

3980

322.1

34.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.547 ± 0.034

0.717 ± 0.007

6.117 ± 0.022

5.416 ± 0.028

2.975 ± 0.017

8.944 ± 0.025

2.073 ± 0.012

3.977 ± 0.015

2.373 ± 0.02

10.322 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.749 ± 0.009

2.203 ± 0.019

5.77 ± 0.024

3.129 ± 0.016

7.309 ± 0.033

5.458 ± 0.021

6.37 ± 0.03

8.672 ± 0.024

1.633 ± 0.012

2.245 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski