Ochrobactrum gallinifaecis
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3370 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A502BSM1|A0A502BSM1_9RHIZ ABC transporter ATP-binding protein OS=Ochrobactrum gallinifaecis OX=215590 GN=FHY56_00275 PE=3 SV=1
MM1 pKa = 7.32 KK2 pKa = 10.21 KK3 pKa = 8.94 WVLSAGFSLVASAALAGEE21 pKa = 4.24 ISIPLPDD28 pKa = 4.84 DD29 pKa = 3.65 VDD31 pKa = 3.78 VTEE34 pKa = 4.65 NSVLYY39 pKa = 10.61 KK40 pKa = 10.9 CGDD43 pKa = 3.58 QNISATYY50 pKa = 9.87 YY51 pKa = 10.43 NAGEE55 pKa = 4.01 NALVQLEE62 pKa = 4.5 MEE64 pKa = 5.21 DD65 pKa = 3.54 NTVIAANVLAGSGARR80 pKa = 11.84 YY81 pKa = 9.96 AGGIYY86 pKa = 9.75 IWWTKK91 pKa = 10.68 GDD93 pKa = 3.63 TADD96 pKa = 5.21 LYY98 pKa = 11.87 NLMDD102 pKa = 5.14 DD103 pKa = 4.71 PEE105 pKa = 4.2 QEE107 pKa = 4.28 KK108 pKa = 10.5 PISCKK113 pKa = 10.63 EE114 pKa = 3.82 EE115 pKa = 3.77
Molecular weight: 12.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.796
IPC2_protein 4.012
IPC_protein 3.935
Toseland 3.745
ProMoST 4.037
Dawson 3.91
Bjellqvist 4.126
Wikipedia 3.834
Rodwell 3.77
Grimsley 3.656
Solomon 3.897
Lehninger 3.846
Nozaki 4.037
DTASelect 4.215
Thurlkill 3.795
EMBOSS 3.834
Sillero 4.05
Patrickios 1.914
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.971
Protein with the highest isoelectric point:
>tr|A0A502BUA7|A0A502BUA7_9RHIZ Pyridoxamine 5'-phosphate oxidase family protein OS=Ochrobactrum gallinifaecis OX=215590 GN=FHY56_04080 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATAGGRR28 pKa = 11.84 KK29 pKa = 8.88 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3370
0
3370
1063554
17
4228
315.6
34.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.926 ± 0.051
0.774 ± 0.012
5.633 ± 0.036
5.849 ± 0.046
3.967 ± 0.028
8.113 ± 0.085
2.024 ± 0.018
6.379 ± 0.031
4.333 ± 0.034
9.811 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.56 ± 0.023
3.407 ± 0.031
4.573 ± 0.033
3.374 ± 0.021
6.195 ± 0.046
6.06 ± 0.039
5.265 ± 0.034
7.071 ± 0.038
1.227 ± 0.015
2.459 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here