Amino acid dipepetide frequency for Microbacterium phage Megan

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.519AlaAla: 16.519 ± 1.251
0.899AlaCys: 0.899 ± 0.267
9.215AlaAsp: 9.215 ± 0.716
8.259AlaGlu: 8.259 ± 0.644
3.09AlaPhe: 3.09 ± 0.392
9.102AlaGly: 9.102 ± 0.784
2.416AlaHis: 2.416 ± 0.45
5.45AlaIle: 5.45 ± 0.969
2.304AlaLys: 2.304 ± 0.37
13.485AlaLeu: 13.485 ± 1.186
3.371AlaMet: 3.371 ± 0.429
3.259AlaAsn: 3.259 ± 0.512
6.799AlaPro: 6.799 ± 0.563
4.832AlaGln: 4.832 ± 0.566
9.664AlaArg: 9.664 ± 0.934
7.81AlaSer: 7.81 ± 0.648
8.259AlaThr: 8.259 ± 0.695
8.596AlaVal: 8.596 ± 0.758
2.978AlaTrp: 2.978 ± 0.363
3.259AlaTyr: 3.259 ± 0.454
0.0AlaXaa: 0.0 ± 0.0
Cys
1.236CysAla: 1.236 ± 0.31
0.337CysCys: 0.337 ± 0.147
1.18CysAsp: 1.18 ± 0.291
0.618CysGlu: 0.618 ± 0.159
0.112CysPhe: 0.112 ± 0.077
1.798CysGly: 1.798 ± 0.373
0.449CysHis: 0.449 ± 0.151
0.281CysIle: 0.281 ± 0.117
0.169CysLys: 0.169 ± 0.092
0.506CysLeu: 0.506 ± 0.129
0.112CysMet: 0.112 ± 0.081
0.169CysAsn: 0.169 ± 0.1
0.787CysPro: 0.787 ± 0.224
0.337CysGln: 0.337 ± 0.128
0.618CysArg: 0.618 ± 0.174
0.787CysSer: 0.787 ± 0.263
0.449CysThr: 0.449 ± 0.201
0.674CysVal: 0.674 ± 0.2
0.169CysTrp: 0.169 ± 0.087
0.281CysTyr: 0.281 ± 0.109
0.0CysXaa: 0.0 ± 0.0
Asp
10.226AspAla: 10.226 ± 0.749
0.787AspCys: 0.787 ± 0.235
6.63AspAsp: 6.63 ± 0.829
5.057AspGlu: 5.057 ± 0.69
1.629AspPhe: 1.629 ± 0.355
7.754AspGly: 7.754 ± 0.677
0.899AspHis: 0.899 ± 0.25
3.034AspIle: 3.034 ± 0.45
1.292AspLys: 1.292 ± 0.301
4.776AspLeu: 4.776 ± 0.576
2.023AspMet: 2.023 ± 0.341
0.955AspAsn: 0.955 ± 0.235
2.922AspPro: 2.922 ± 0.447
1.236AspGln: 1.236 ± 0.298
3.708AspArg: 3.708 ± 0.543
3.652AspSer: 3.652 ± 0.449
4.045AspThr: 4.045 ± 0.595
5.113AspVal: 5.113 ± 0.503
1.461AspTrp: 1.461 ± 0.293
1.292AspTyr: 1.292 ± 0.278
0.0AspXaa: 0.0 ± 0.0
Glu
9.046GluAla: 9.046 ± 0.955
0.674GluCys: 0.674 ± 0.2
4.214GluAsp: 4.214 ± 0.553
4.439GluGlu: 4.439 ± 0.628
1.461GluPhe: 1.461 ± 0.27
4.27GluGly: 4.27 ± 0.534
3.259GluHis: 3.259 ± 0.447
0.787GluIle: 0.787 ± 0.259
1.124GluLys: 1.124 ± 0.234
3.259GluLeu: 3.259 ± 0.543
1.011GluMet: 1.011 ± 0.246
1.011GluAsn: 1.011 ± 0.313
4.326GluPro: 4.326 ± 0.518
2.416GluGln: 2.416 ± 0.336
7.023GluArg: 7.023 ± 0.763
2.079GluSer: 2.079 ± 0.347
2.585GluThr: 2.585 ± 0.377
6.068GluVal: 6.068 ± 0.487
2.023GluTrp: 2.023 ± 0.375
1.629GluTyr: 1.629 ± 0.299
0.0GluXaa: 0.0 ± 0.0
Phe
2.585PheAla: 2.585 ± 0.389
0.281PheCys: 0.281 ± 0.116
1.742PheAsp: 1.742 ± 0.299
1.18PheGlu: 1.18 ± 0.244
0.337PhePhe: 0.337 ± 0.113
2.36PheGly: 2.36 ± 0.382
0.337PheHis: 0.337 ± 0.105
1.236PheIle: 1.236 ± 0.246
0.787PheLys: 0.787 ± 0.194
1.348PheLeu: 1.348 ± 0.372
0.843PheMet: 0.843 ± 0.225
0.843PheAsn: 0.843 ± 0.25
0.955PhePro: 0.955 ± 0.268
0.787PheGln: 0.787 ± 0.17
1.461PheArg: 1.461 ± 0.329
1.348PheSer: 1.348 ± 0.219
1.686PheThr: 1.686 ± 0.281
1.91PheVal: 1.91 ± 0.358
0.506PheTrp: 0.506 ± 0.188
0.393PheTyr: 0.393 ± 0.127
0.0PheXaa: 0.0 ± 0.0
Gly
10.338GlyAla: 10.338 ± 0.851
0.787GlyCys: 0.787 ± 0.216
5.619GlyAsp: 5.619 ± 0.594
3.877GlyGlu: 3.877 ± 0.527
2.191GlyPhe: 2.191 ± 0.302
7.36GlyGly: 7.36 ± 0.736
2.304GlyHis: 2.304 ± 0.432
4.439GlyIle: 4.439 ± 0.663
2.023GlyLys: 2.023 ± 0.335
7.248GlyLeu: 7.248 ± 0.497
2.247GlyMet: 2.247 ± 0.331
2.416GlyAsn: 2.416 ± 0.388
3.708GlyPro: 3.708 ± 0.542
2.809GlyGln: 2.809 ± 0.41
4.045GlyArg: 4.045 ± 0.442
6.461GlySer: 6.461 ± 0.763
6.237GlyThr: 6.237 ± 0.731
6.574GlyVal: 6.574 ± 0.633
2.247GlyTrp: 2.247 ± 0.326
2.641GlyTyr: 2.641 ± 0.41
0.0GlyXaa: 0.0 ± 0.0
His
2.809HisAla: 2.809 ± 0.481
0.225HisCys: 0.225 ± 0.097
1.742HisAsp: 1.742 ± 0.292
1.461HisGlu: 1.461 ± 0.351
0.618HisPhe: 0.618 ± 0.184
2.416HisGly: 2.416 ± 0.418
0.449HisHis: 0.449 ± 0.146
0.843HisIle: 0.843 ± 0.258
0.281HisLys: 0.281 ± 0.109
2.023HisLeu: 2.023 ± 0.349
0.899HisMet: 0.899 ± 0.224
0.506HisAsn: 0.506 ± 0.156
1.068HisPro: 1.068 ± 0.227
0.169HisGln: 0.169 ± 0.107
1.405HisArg: 1.405 ± 0.336
0.787HisSer: 0.787 ± 0.224
1.798HisThr: 1.798 ± 0.334
2.191HisVal: 2.191 ± 0.413
0.225HisTrp: 0.225 ± 0.106
0.449HisTyr: 0.449 ± 0.167
0.0HisXaa: 0.0 ± 0.0
Ile
5.562IleAla: 5.562 ± 0.564
0.506IleCys: 0.506 ± 0.192
3.09IleAsp: 3.09 ± 0.555
3.427IleGlu: 3.427 ± 0.563
0.674IlePhe: 0.674 ± 0.201
3.877IleGly: 3.877 ± 0.613
0.73IleHis: 0.73 ± 0.211
1.91IleIle: 1.91 ± 0.584
0.899IleLys: 0.899 ± 0.206
2.247IleLeu: 2.247 ± 0.522
0.787IleMet: 0.787 ± 0.214
1.011IleAsn: 1.011 ± 0.303
2.641IlePro: 2.641 ± 0.661
1.686IleGln: 1.686 ± 0.388
2.135IleArg: 2.135 ± 0.389
1.517IleSer: 1.517 ± 0.333
4.383IleThr: 4.383 ± 0.52
3.54IleVal: 3.54 ± 0.499
0.787IleTrp: 0.787 ± 0.176
0.787IleTyr: 0.787 ± 0.182
0.0IleXaa: 0.0 ± 0.0
Lys
3.708LysAla: 3.708 ± 0.483
0.225LysCys: 0.225 ± 0.112
1.068LysAsp: 1.068 ± 0.283
1.292LysGlu: 1.292 ± 0.295
0.618LysPhe: 0.618 ± 0.182
2.304LysGly: 2.304 ± 0.372
0.281LysHis: 0.281 ± 0.106
0.899LysIle: 0.899 ± 0.216
0.506LysLys: 0.506 ± 0.21
0.225LysLeu: 0.225 ± 0.103
0.169LysMet: 0.169 ± 0.094
0.337LysAsn: 0.337 ± 0.122
1.461LysPro: 1.461 ± 0.313
0.618LysGln: 0.618 ± 0.195
2.416LysArg: 2.416 ± 0.393
0.618LysSer: 0.618 ± 0.201
0.787LysThr: 0.787 ± 0.159
2.135LysVal: 2.135 ± 0.453
0.281LysTrp: 0.281 ± 0.111
0.337LysTyr: 0.337 ± 0.139
0.0LysXaa: 0.0 ± 0.0
Leu
9.72LeuAla: 9.72 ± 0.781
0.674LeuCys: 0.674 ± 0.236
6.124LeuAsp: 6.124 ± 0.529
3.764LeuGlu: 3.764 ± 0.419
1.124LeuPhe: 1.124 ± 0.3
7.192LeuGly: 7.192 ± 0.707
1.236LeuHis: 1.236 ± 0.212
3.989LeuIle: 3.989 ± 0.754
0.787LeuLys: 0.787 ± 0.166
6.012LeuLeu: 6.012 ± 0.745
1.91LeuMet: 1.91 ± 0.332
1.18LeuAsn: 1.18 ± 0.288
4.72LeuPro: 4.72 ± 0.636
1.854LeuGln: 1.854 ± 0.342
4.776LeuArg: 4.776 ± 0.476
5.675LeuSer: 5.675 ± 0.665
7.192LeuThr: 7.192 ± 0.728
6.742LeuVal: 6.742 ± 0.669
1.573LeuTrp: 1.573 ± 0.353
1.236LeuTyr: 1.236 ± 0.266
0.0LeuXaa: 0.0 ± 0.0
Met
3.146MetAla: 3.146 ± 0.5
0.112MetCys: 0.112 ± 0.079
1.068MetAsp: 1.068 ± 0.27
1.18MetGlu: 1.18 ± 0.197
0.562MetPhe: 0.562 ± 0.197
1.91MetGly: 1.91 ± 0.349
0.562MetHis: 0.562 ± 0.167
0.843MetIle: 0.843 ± 0.224
0.281MetLys: 0.281 ± 0.132
2.697MetLeu: 2.697 ± 0.362
0.562MetMet: 0.562 ± 0.18
0.73MetAsn: 0.73 ± 0.196
1.573MetPro: 1.573 ± 0.332
0.73MetGln: 0.73 ± 0.195
2.416MetArg: 2.416 ± 0.412
2.585MetSer: 2.585 ± 0.4
2.36MetThr: 2.36 ± 0.347
1.18MetVal: 1.18 ± 0.243
0.112MetTrp: 0.112 ± 0.071
0.449MetTyr: 0.449 ± 0.154
0.0MetXaa: 0.0 ± 0.0
Asn
2.922AsnAla: 2.922 ± 0.413
0.281AsnCys: 0.281 ± 0.122
1.236AsnAsp: 1.236 ± 0.302
1.068AsnGlu: 1.068 ± 0.231
0.337AsnPhe: 0.337 ± 0.15
2.191AsnGly: 2.191 ± 0.36
0.506AsnHis: 0.506 ± 0.153
0.899AsnIle: 0.899 ± 0.261
0.506AsnLys: 0.506 ± 0.183
1.854AsnLeu: 1.854 ± 0.358
0.506AsnMet: 0.506 ± 0.137
0.618AsnAsn: 0.618 ± 0.243
1.573AsnPro: 1.573 ± 0.333
0.393AsnGln: 0.393 ± 0.155
1.517AsnArg: 1.517 ± 0.282
1.292AsnSer: 1.292 ± 0.263
1.686AsnThr: 1.686 ± 0.347
2.023AsnVal: 2.023 ± 0.325
0.562AsnTrp: 0.562 ± 0.226
0.506AsnTyr: 0.506 ± 0.186
0.0AsnXaa: 0.0 ± 0.0
Pro
7.023ProAla: 7.023 ± 0.7
0.899ProCys: 0.899 ± 0.22
3.708ProAsp: 3.708 ± 0.466
4.888ProGlu: 4.888 ± 0.502
1.629ProPhe: 1.629 ± 0.282
3.989ProGly: 3.989 ± 0.463
1.405ProHis: 1.405 ± 0.287
2.023ProIle: 2.023 ± 0.365
1.798ProLys: 1.798 ± 0.393
4.214ProLeu: 4.214 ± 0.376
1.517ProMet: 1.517 ± 0.329
1.236ProAsn: 1.236 ± 0.246
4.439ProPro: 4.439 ± 0.507
1.686ProGln: 1.686 ± 0.398
2.079ProArg: 2.079 ± 0.365
3.764ProSer: 3.764 ± 0.403
5.169ProThr: 5.169 ± 0.59
4.776ProVal: 4.776 ± 0.516
0.899ProTrp: 0.899 ± 0.223
1.405ProTyr: 1.405 ± 0.3
0.0ProXaa: 0.0 ± 0.0
Gln
5.001GlnAla: 5.001 ± 0.415
0.169GlnCys: 0.169 ± 0.097
1.798GlnAsp: 1.798 ± 0.368
1.967GlnGlu: 1.967 ± 0.324
0.506GlnPhe: 0.506 ± 0.203
2.36GlnGly: 2.36 ± 0.388
0.787GlnHis: 0.787 ± 0.217
1.629GlnIle: 1.629 ± 0.304
0.562GlnLys: 0.562 ± 0.202
1.011GlnLeu: 1.011 ± 0.411
1.18GlnMet: 1.18 ± 0.3
1.011GlnAsn: 1.011 ± 0.278
1.854GlnPro: 1.854 ± 0.321
1.011GlnGln: 1.011 ± 0.31
2.36GlnArg: 2.36 ± 0.368
1.798GlnSer: 1.798 ± 0.326
1.967GlnThr: 1.967 ± 0.345
1.405GlnVal: 1.405 ± 0.259
0.449GlnTrp: 0.449 ± 0.161
0.787GlnTyr: 0.787 ± 0.173
0.0GlnXaa: 0.0 ± 0.0
Arg
8.372ArgAla: 8.372 ± 0.85
0.955ArgCys: 0.955 ± 0.264
4.045ArgAsp: 4.045 ± 0.524
5.001ArgGlu: 5.001 ± 0.647
2.36ArgPhe: 2.36 ± 0.444
5.001ArgGly: 5.001 ± 0.47
1.686ArgHis: 1.686 ± 0.297
2.585ArgIle: 2.585 ± 0.429
1.742ArgLys: 1.742 ± 0.285
6.518ArgLeu: 6.518 ± 0.643
2.023ArgMet: 2.023 ± 0.325
1.292ArgAsn: 1.292 ± 0.297
2.978ArgPro: 2.978 ± 0.45
2.304ArgGln: 2.304 ± 0.439
6.518ArgArg: 6.518 ± 0.696
3.933ArgSer: 3.933 ± 0.485
3.877ArgThr: 3.877 ± 0.539
5.169ArgVal: 5.169 ± 0.642
2.135ArgTrp: 2.135 ± 0.338
2.304ArgTyr: 2.304 ± 0.383
0.0ArgXaa: 0.0 ± 0.0
Ser
7.978SerAla: 7.978 ± 0.64
0.674SerCys: 0.674 ± 0.167
4.663SerAsp: 4.663 ± 0.569
3.146SerGlu: 3.146 ± 0.395
1.91SerPhe: 1.91 ± 0.371
5.394SerGly: 5.394 ± 0.483
1.068SerHis: 1.068 ± 0.251
2.753SerIle: 2.753 ± 0.369
1.348SerLys: 1.348 ± 0.302
4.776SerLeu: 4.776 ± 0.413
1.461SerMet: 1.461 ± 0.309
1.292SerAsn: 1.292 ± 0.303
2.809SerPro: 2.809 ± 0.4
1.573SerGln: 1.573 ± 0.332
3.764SerArg: 3.764 ± 0.487
2.528SerSer: 2.528 ± 0.381
4.27SerThr: 4.27 ± 0.551
3.764SerVal: 3.764 ± 0.511
1.686SerTrp: 1.686 ± 0.32
1.292SerTyr: 1.292 ± 0.281
0.0SerXaa: 0.0 ± 0.0
Thr
8.821ThrAla: 8.821 ± 0.966
1.068ThrCys: 1.068 ± 0.27
4.607ThrAsp: 4.607 ± 0.524
4.102ThrGlu: 4.102 ± 0.55
1.236ThrPhe: 1.236 ± 0.264
5.843ThrGly: 5.843 ± 0.778
1.236ThrHis: 1.236 ± 0.269
3.09ThrIle: 3.09 ± 0.479
1.405ThrLys: 1.405 ± 0.326
6.405ThrLeu: 6.405 ± 0.656
1.517ThrMet: 1.517 ± 0.288
1.854ThrAsn: 1.854 ± 0.312
6.124ThrPro: 6.124 ± 0.689
2.304ThrGln: 2.304 ± 0.417
4.607ThrArg: 4.607 ± 0.529
4.495ThrSer: 4.495 ± 0.555
4.607ThrThr: 4.607 ± 0.611
4.832ThrVal: 4.832 ± 0.497
1.348ThrTrp: 1.348 ± 0.338
1.124ThrTyr: 1.124 ± 0.327
0.0ThrXaa: 0.0 ± 0.0
Val
9.552ValAla: 9.552 ± 0.686
0.787ValCys: 0.787 ± 0.218
3.315ValAsp: 3.315 ± 0.415
4.944ValGlu: 4.944 ± 0.454
1.629ValPhe: 1.629 ± 0.392
6.574ValGly: 6.574 ± 0.571
1.967ValHis: 1.967 ± 0.382
3.989ValIle: 3.989 ± 0.448
1.798ValLys: 1.798 ± 0.318
5.506ValLeu: 5.506 ± 0.604
1.798ValMet: 1.798 ± 0.343
1.798ValAsn: 1.798 ± 0.289
5.675ValPro: 5.675 ± 0.544
1.798ValGln: 1.798 ± 0.274
6.518ValArg: 6.518 ± 0.651
4.214ValSer: 4.214 ± 0.547
5.731ValThr: 5.731 ± 0.527
6.686ValVal: 6.686 ± 0.655
1.742ValTrp: 1.742 ± 0.27
1.292ValTyr: 1.292 ± 0.224
0.0ValXaa: 0.0 ± 0.0
Trp
2.36TrpAla: 2.36 ± 0.294
0.506TrpCys: 0.506 ± 0.153
1.629TrpAsp: 1.629 ± 0.334
1.967TrpGlu: 1.967 ± 0.287
0.506TrpPhe: 0.506 ± 0.189
1.348TrpGly: 1.348 ± 0.254
0.562TrpHis: 0.562 ± 0.178
0.506TrpIle: 0.506 ± 0.2
0.393TrpLys: 0.393 ± 0.149
1.967TrpLeu: 1.967 ± 0.318
0.506TrpMet: 0.506 ± 0.159
0.506TrpAsn: 0.506 ± 0.145
0.73TrpPro: 0.73 ± 0.234
0.449TrpGln: 0.449 ± 0.151
1.91TrpArg: 1.91 ± 0.311
1.573TrpSer: 1.573 ± 0.327
1.573TrpThr: 1.573 ± 0.329
1.854TrpVal: 1.854 ± 0.364
0.393TrpTrp: 0.393 ± 0.162
0.674TrpTyr: 0.674 ± 0.2
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.641TyrAla: 2.641 ± 0.415
0.225TyrCys: 0.225 ± 0.137
1.798TyrAsp: 1.798 ± 0.331
1.068TyrGlu: 1.068 ± 0.242
0.393TyrPhe: 0.393 ± 0.149
2.528TyrGly: 2.528 ± 0.558
0.225TyrHis: 0.225 ± 0.09
1.011TyrIle: 1.011 ± 0.237
0.281TyrLys: 0.281 ± 0.129
1.405TyrLeu: 1.405 ± 0.279
0.562TyrMet: 0.562 ± 0.199
0.449TyrAsn: 0.449 ± 0.15
1.405TyrPro: 1.405 ± 0.264
0.618TyrGln: 0.618 ± 0.146
1.91TyrArg: 1.91 ± 0.329
1.236TyrSer: 1.236 ± 0.262
1.967TyrThr: 1.967 ± 0.434
2.079TyrVal: 2.079 ± 0.302
0.337TyrTrp: 0.337 ± 0.128
0.73TyrTyr: 0.73 ± 0.191
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 90 proteins (17799 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski