Betaproteobacteria bacterium SCN2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; unclassified Betaproteobacteria

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2629 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H9Y8K1|A0A6H9Y8K1_9PROT C-type cytochrome OS=Betaproteobacteria bacterium SCN2 OX=2651628 GN=F8A87_00185 PE=4 SV=1
MM1 pKa = 7.83SDD3 pKa = 3.0TPEE6 pKa = 4.11YY7 pKa = 8.7KK8 pKa = 9.19TWMCLLCGFIYY19 pKa = 10.51DD20 pKa = 4.65EE21 pKa = 4.44EE22 pKa = 5.07KK23 pKa = 10.69GWPQDD28 pKa = 3.53GIAPGTRR35 pKa = 11.84WEE37 pKa = 4.3DD38 pKa = 3.35VPMNWTCPDD47 pKa = 3.26CGGRR51 pKa = 11.84KK52 pKa = 8.92EE53 pKa = 4.37DD54 pKa = 3.97FEE56 pKa = 4.79MIAVV60 pKa = 4.03

Molecular weight: 6.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H9Y1Q2|A0A6H9Y1Q2_9PROT TolC family protein OS=Betaproteobacteria bacterium SCN2 OX=2651628 GN=F8A87_10605 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.86GRR39 pKa = 11.84HH40 pKa = 4.92RR41 pKa = 11.84LAVV44 pKa = 3.37

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2629

0

2629

806251

32

1917

306.7

33.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.82 ± 0.071

0.933 ± 0.016

5.354 ± 0.039

6.224 ± 0.049

3.644 ± 0.031

7.996 ± 0.045

2.379 ± 0.024

4.883 ± 0.033

3.955 ± 0.042

11.002 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.586 ± 0.025

2.814 ± 0.027

4.949 ± 0.033

3.721 ± 0.029

6.901 ± 0.047

5.215 ± 0.031

4.621 ± 0.03

7.089 ± 0.036

1.416 ± 0.023

2.501 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski