Sewage-associated gemycircularvirus 6

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus sewopo3; Sewage derived gemycircularvirus 3

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7CL87|A0A0A7CL87_9VIRU Replication-associated protein OS=Sewage-associated gemycircularvirus 6 OX=1519401 PE=3 SV=1
MM1 pKa = 7.62SSFSFHH7 pKa = 6.37ARR9 pKa = 11.84YY10 pKa = 9.75ALLTYY15 pKa = 8.93AQCGDD20 pKa = 4.24LCPFTIVDD28 pKa = 4.65LLSTMGAEE36 pKa = 4.28CIIGRR41 pKa = 11.84EE42 pKa = 4.01HH43 pKa = 6.42HH44 pKa = 6.33QDD46 pKa = 3.17GGIHH50 pKa = 5.88LHH52 pKa = 5.67VFVDD56 pKa = 4.46FGRR59 pKa = 11.84KK60 pKa = 7.79YY61 pKa = 10.01RR62 pKa = 11.84SRR64 pKa = 11.84RR65 pKa = 11.84ADD67 pKa = 3.3TFDD70 pKa = 3.1VGGFHH75 pKa = 7.34PNISQSYY82 pKa = 6.35GTPEE86 pKa = 3.51KK87 pKa = 10.88GYY89 pKa = 10.89DD90 pKa = 3.65YY91 pKa = 11.08ACKK94 pKa = 10.67DD95 pKa = 3.3GDD97 pKa = 4.13VVAGGLDD104 pKa = 3.18RR105 pKa = 11.84PVPTEE110 pKa = 3.67GRR112 pKa = 11.84GGDD115 pKa = 3.77SKK117 pKa = 9.73THH119 pKa = 6.28SIWSQITSATTRR131 pKa = 11.84EE132 pKa = 4.34SFWDD136 pKa = 3.95LVHH139 pKa = 7.71DD140 pKa = 5.62LDD142 pKa = 4.59PKK144 pKa = 10.56SAVTCFTQLQKK155 pKa = 11.25YY156 pKa = 9.43CDD158 pKa = 3.1WKK160 pKa = 11.12YY161 pKa = 10.72RR162 pKa = 11.84YY163 pKa = 9.41CPPAYY168 pKa = 9.27EE169 pKa = 4.48SPAGARR175 pKa = 11.84FRR177 pKa = 11.84NDD179 pKa = 2.89TSDD182 pKa = 4.64GRR184 pKa = 11.84GDD186 pKa = 3.27WLLQSGIGGARR197 pKa = 11.84VGRR200 pKa = 11.84VKK202 pKa = 10.87SLVLYY207 pKa = 10.59GPSQTGKK214 pKa = 6.66TTWARR219 pKa = 11.84SLGAHH224 pKa = 7.37IYY226 pKa = 9.89QVGLLSGSEE235 pKa = 4.17CMKK238 pKa = 11.07APDD241 pKa = 3.17VEE243 pKa = 4.49YY244 pKa = 10.88AVFDD248 pKa = 5.02DD249 pKa = 4.07IRR251 pKa = 11.84GGMKK255 pKa = 9.69FFPSFKK261 pKa = 9.92EE262 pKa = 3.7WLGCQPHH269 pKa = 5.71VCVKK273 pKa = 10.06EE274 pKa = 4.09LYY276 pKa = 9.83RR277 pKa = 11.84EE278 pKa = 3.93PRR280 pKa = 11.84VIEE283 pKa = 3.94WGKK286 pKa = 8.54PAIWCSNADD295 pKa = 3.41PRR297 pKa = 11.84DD298 pKa = 3.73DD299 pKa = 3.64MSYY302 pKa = 11.47CDD304 pKa = 4.3VQWMEE309 pKa = 4.25ANCTFIEE316 pKa = 4.19ITEE319 pKa = 4.35KK320 pKa = 11.12LLDD323 pKa = 3.58WEE325 pKa = 4.44

Molecular weight: 36.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7CL87|A0A0A7CL87_9VIRU Replication-associated protein OS=Sewage-associated gemycircularvirus 6 OX=1519401 PE=3 SV=1
MM1 pKa = 7.02THH3 pKa = 7.41PSFICQQPNMIRR15 pKa = 11.84SRR17 pKa = 11.84RR18 pKa = 11.84TRR20 pKa = 11.84SRR22 pKa = 11.84RR23 pKa = 11.84SVRR26 pKa = 11.84PRR28 pKa = 11.84RR29 pKa = 11.84PLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ITKK38 pKa = 9.73RR39 pKa = 11.84RR40 pKa = 11.84PTVKK44 pKa = 10.16RR45 pKa = 11.84AISKK49 pKa = 10.22KK50 pKa = 9.82RR51 pKa = 11.84ILNITSRR58 pKa = 11.84KK59 pKa = 9.05KK60 pKa = 10.33RR61 pKa = 11.84DD62 pKa = 3.84VMNQWSNTTADD73 pKa = 4.15GGSKK77 pKa = 10.34PITNGIVTLAGPVGGYY93 pKa = 8.74FYY95 pKa = 10.34WQATARR101 pKa = 11.84TLDD104 pKa = 3.41QGPGQIGIVSDD115 pKa = 3.56EE116 pKa = 4.29ATRR119 pKa = 11.84TATTCFMRR127 pKa = 11.84GLSEE131 pKa = 5.64RR132 pKa = 11.84IDD134 pKa = 3.35IQTNSSLPWRR144 pKa = 11.84WRR146 pKa = 11.84RR147 pKa = 11.84ICITTKK153 pKa = 10.67DD154 pKa = 3.39NSYY157 pKa = 11.23DD158 pKa = 3.72HH159 pKa = 7.37SDD161 pKa = 3.43LGALQAYY168 pKa = 8.22GGWLLSSAGIVRR180 pKa = 11.84PWVNSYY186 pKa = 10.88INNNGTTQAGQWVDD200 pKa = 3.59LFEE203 pKa = 4.87GAGNVDD209 pKa = 3.11WADD212 pKa = 3.52VNSAKK217 pKa = 10.03IDD219 pKa = 3.36TRR221 pKa = 11.84RR222 pKa = 11.84IDD224 pKa = 3.26LHH226 pKa = 6.5YY227 pKa = 11.16DD228 pKa = 2.93KK229 pKa = 10.65TITINSGNSSGVFRR243 pKa = 11.84TYY245 pKa = 10.77RR246 pKa = 11.84RR247 pKa = 11.84WHH249 pKa = 6.37PMNKK253 pKa = 9.52NLVYY257 pKa = 10.86DD258 pKa = 4.63DD259 pKa = 4.9DD260 pKa = 4.58QDD262 pKa = 3.76GGKK265 pKa = 8.88MDD267 pKa = 3.99SSHH270 pKa = 7.38LSVTDD275 pKa = 3.18KK276 pKa = 11.14RR277 pKa = 11.84GMGNYY282 pKa = 8.97IVLDD286 pKa = 3.85FFQPHH291 pKa = 6.51ASATSADD298 pKa = 3.96RR299 pKa = 11.84LILRR303 pKa = 11.84SNSALFWHH311 pKa = 6.89EE312 pKa = 3.9KK313 pKa = 9.73

Molecular weight: 35.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

638

313

325

319.0

36.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.956 ± 0.138

2.508 ± 1.041

7.524 ± 0.332

3.135 ± 1.248

3.605 ± 0.705

8.777 ± 0.746

2.665 ± 0.288

5.643 ± 0.931

4.545 ± 0.049

6.113 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.038 ± 0.134

3.605 ± 1.656

4.389 ± 0.373

3.605 ± 0.154

8.621 ± 1.721

8.15 ± 0.534

6.897 ± 0.947

5.329 ± 0.361

3.135 ± 0.04

3.762 ± 0.596
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski