Leptonychotes weddellii papillomavirus 3
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I8B2P6|A0A2I8B2P6_9PAPI Replication protein E1 OS=Leptonychotes weddellii papillomavirus 3 OX=2077304 GN=E1 PE=3 SV=1
MM1 pKa = 7.61 IGTEE5 pKa = 4.13 PTLKK9 pKa = 10.68 DD10 pKa = 3.16 IVLGEE15 pKa = 4.18 QPVPADD21 pKa = 4.33 DD22 pKa = 3.97 LWCYY26 pKa = 10.94 EE27 pKa = 4.08 EE28 pKa = 5.38 LPPEE32 pKa = 3.94 EE33 pKa = 5.1 AEE35 pKa = 4.89 RR36 pKa = 11.84 SDD38 pKa = 3.35 QAPYY42 pKa = 9.93 RR43 pKa = 11.84 VHH45 pKa = 7.17 ASCGFCGRR53 pKa = 11.84 GIEE56 pKa = 4.26 LVVLSSRR63 pKa = 11.84 VAIRR67 pKa = 11.84 TLQHH71 pKa = 6.84 LLLEE75 pKa = 4.71 NLDD78 pKa = 4.11 IVCPDD83 pKa = 3.84 CARR86 pKa = 11.84 TRR88 pKa = 11.84 ALHH91 pKa = 6.35 HH92 pKa = 6.63 GGG94 pKa = 3.46
Molecular weight: 10.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.729
IPC2_protein 4.889
IPC_protein 4.762
Toseland 4.673
ProMoST 4.889
Dawson 4.736
Bjellqvist 4.876
Wikipedia 4.596
Rodwell 4.647
Grimsley 4.596
Solomon 4.736
Lehninger 4.685
Nozaki 4.851
DTASelect 4.978
Thurlkill 4.685
EMBOSS 4.635
Sillero 4.927
Patrickios 3.35
IPC_peptide 4.736
IPC2_peptide 4.914
IPC2.peptide.svr19 4.862
Protein with the highest isoelectric point:
>tr|A0A2I8B2Q2|A0A2I8B2Q2_9PAPI Minor capsid protein L2 OS=Leptonychotes weddellii papillomavirus 3 OX=2077304 GN=L2 PE=3 SV=1
MM1 pKa = 7.41 VEE3 pKa = 3.97 NLAKK7 pKa = 10.65 RR8 pKa = 11.84 LDD10 pKa = 3.75 LVRR13 pKa = 11.84 DD14 pKa = 3.82 GLLDD18 pKa = 4.06 LYY20 pKa = 10.2 EE21 pKa = 4.86 RR22 pKa = 11.84 CDD24 pKa = 4.53 DD25 pKa = 4.02 SLEE28 pKa = 4.23 SQLKK32 pKa = 8.81 HH33 pKa = 4.91 WQLLRR38 pKa = 11.84 QEE40 pKa = 4.05 YY41 pKa = 10.43 AILYY45 pKa = 6.48 FARR48 pKa = 11.84 RR49 pKa = 11.84 SGIIRR54 pKa = 11.84 VGCQPVPPAQVSRR67 pKa = 11.84 EE68 pKa = 3.86 RR69 pKa = 11.84 ARR71 pKa = 11.84 DD72 pKa = 3.72 AIEE75 pKa = 3.85 VHH77 pKa = 6.95 LMLQSLQQSNFCNEE91 pKa = 3.7 PWTLADD97 pKa = 3.75 TSLEE101 pKa = 4.13 RR102 pKa = 11.84 YY103 pKa = 7.53 KK104 pKa = 10.53 TEE106 pKa = 4.97 PQGCWKK112 pKa = 10.22 KK113 pKa = 9.83 NPEE116 pKa = 4.2 MVEE119 pKa = 3.58 VRR121 pKa = 11.84 YY122 pKa = 10.89 DD123 pKa = 3.57 GDD125 pKa = 3.65 RR126 pKa = 11.84 EE127 pKa = 4.0 NATLHH132 pKa = 5.91 TLWQDD137 pKa = 2.71 IYY139 pKa = 11.04 YY140 pKa = 10.28 RR141 pKa = 11.84 NSDD144 pKa = 3.81 GVWCKK149 pKa = 7.72 THH151 pKa = 5.99 GQVDD155 pKa = 3.76 GHH157 pKa = 6.17 GLFYY161 pKa = 10.91 LDD163 pKa = 4.39 NGCKK167 pKa = 9.56 RR168 pKa = 11.84 YY169 pKa = 10.03 YY170 pKa = 11.01 VYY172 pKa = 10.53 FADD175 pKa = 4.62 DD176 pKa = 3.3 AKK178 pKa = 10.7 KK179 pKa = 10.51 YY180 pKa = 9.54 SRR182 pKa = 11.84 SGIWEE187 pKa = 3.82 VEE189 pKa = 4.17 YY190 pKa = 11.01 KK191 pKa = 10.91 NEE193 pKa = 3.96 ILSPLDD199 pKa = 3.47 SVTSTTAPAHH209 pKa = 5.22 VCRR212 pKa = 11.84 PPEE215 pKa = 4.52 GPARR219 pKa = 11.84 TPARR223 pKa = 11.84 GSAGRR228 pKa = 11.84 LSTPPEE234 pKa = 4.05 PRR236 pKa = 11.84 HH237 pKa = 5.71 TPWSPQVSTSRR248 pKa = 11.84 ADD250 pKa = 3.65 VPDD253 pKa = 3.59 TTSPLGGPVPALAAQPTLPTAVSRR277 pKa = 11.84 SWKK280 pKa = 9.39 SATRR284 pKa = 11.84 RR285 pKa = 11.84 HH286 pKa = 5.74 RR287 pKa = 11.84 RR288 pKa = 11.84 CLGPVTAATPGATDD302 pKa = 3.36 TRR304 pKa = 11.84 GGAAAAPYY312 pKa = 9.87 SAEE315 pKa = 4.56 APPSSPTPAEE325 pKa = 4.16 GPEE328 pKa = 4.08 RR329 pKa = 11.84 EE330 pKa = 4.76 PEE332 pKa = 4.05 RR333 pKa = 11.84 QPADD337 pKa = 3.52 PGRR340 pKa = 11.84 RR341 pKa = 11.84 LPPAARR347 pKa = 11.84 PNRR350 pKa = 11.84 GRR352 pKa = 11.84 PGRR355 pKa = 11.84 LLRR358 pKa = 11.84 GTGDD362 pKa = 3.71 CSPALAVLRR371 pKa = 11.84 GSGNVLKK378 pKa = 10.53 CYY380 pKa = 10.26 RR381 pKa = 11.84 YY382 pKa = 9.86 RR383 pKa = 11.84 IKK385 pKa = 10.59 KK386 pKa = 7.63 KK387 pKa = 9.47 HH388 pKa = 5.6 RR389 pKa = 11.84 RR390 pKa = 11.84 LFRR393 pKa = 11.84 AVSTTWHH400 pKa = 5.2 WTGEE404 pKa = 4.21 DD405 pKa = 3.67 GADD408 pKa = 3.37 RR409 pKa = 11.84 AGEE412 pKa = 3.83 ARR414 pKa = 11.84 LLVWFEE420 pKa = 4.19 NPAQRR425 pKa = 11.84 SEE427 pKa = 4.23 FFEE430 pKa = 4.49 SVPMPPSVSAYY441 pKa = 9.82 EE442 pKa = 4.15 GLGGLL447 pKa = 4.48
Molecular weight: 49.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.269
IPC2_protein 8.273
IPC_protein 8.185
Toseland 8.083
ProMoST 8.653
Dawson 8.873
Bjellqvist 9.092
Wikipedia 8.902
Rodwell 8.902
Grimsley 8.068
Solomon 8.96
Lehninger 8.946
Nozaki 9.194
DTASelect 8.858
Thurlkill 8.902
EMBOSS 9.033
Sillero 9.18
Patrickios 4.507
IPC_peptide 8.96
IPC2_peptide 8.17
IPC2.peptide.svr19 8.086
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2336
94
632
389.3
43.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.449 ± 0.476
2.568 ± 0.724
5.437 ± 0.21
6.507 ± 0.54
3.81 ± 0.525
7.705 ± 0.758
2.226 ± 0.183
3.767 ± 0.509
3.938 ± 0.649
8.69 ± 0.801
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.584 ± 0.524
3.682 ± 0.753
6.807 ± 1.007
3.981 ± 0.408
7.363 ± 0.839
7.834 ± 0.651
5.95 ± 0.477
5.95 ± 0.764
1.627 ± 0.42
3.082 ± 0.325
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here