Bordetella virus LK3
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D0W900|A0A2D0W900_9CAUD Putative structural protein OS=Bordetella virus LK3 OX=1926943 GN=LK3_22 PE=4 SV=1
MM1 pKa = 7.47 TPMDD5 pKa = 5.03 PEE7 pKa = 4.24 NLPPPIATLGPDD19 pKa = 3.0 VTFYY23 pKa = 11.54 DD24 pKa = 4.63 DD25 pKa = 4.11 GDD27 pKa = 3.83 VTEE30 pKa = 4.81 GLTYY34 pKa = 9.98 YY35 pKa = 10.72 YY36 pKa = 10.51 RR37 pKa = 11.84 VGAFNAVRR45 pKa = 11.84 EE46 pKa = 4.5 VVSEE50 pKa = 4.0 EE51 pKa = 4.24 LEE53 pKa = 4.37 APADD57 pKa = 3.81 PNAPQARR64 pKa = 11.84 LIWRR68 pKa = 11.84 ILIQDD73 pKa = 3.72 TRR75 pKa = 11.84 SGDD78 pKa = 5.05 DD79 pKa = 3.36 FACWVSQIQMRR90 pKa = 11.84 VLGGPNLAVGGSAYY104 pKa = 10.42 ASSFYY109 pKa = 10.14 STPEE113 pKa = 3.27 DD114 pKa = 3.44 WGPGRR119 pKa = 11.84 AFDD122 pKa = 4.59 GSAATGWSCDD132 pKa = 3.14 RR133 pKa = 11.84 DD134 pKa = 3.58 APLPQWIAYY143 pKa = 7.34 EE144 pKa = 4.13 LTSPAALTEE153 pKa = 3.9 ISIQAGNNAGRR164 pKa = 11.84 AARR167 pKa = 11.84 APRR170 pKa = 11.84 TFQIQSSTDD179 pKa = 3.12 GTIWEE184 pKa = 4.51 TRR186 pKa = 11.84 FTATDD191 pKa = 3.63 EE192 pKa = 4.42 PAWDD196 pKa = 3.94 PGEE199 pKa = 3.8 IRR201 pKa = 11.84 TYY203 pKa = 11.5 AFF205 pKa = 3.72
Molecular weight: 22.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.171
IPC2_protein 4.075
IPC_protein 4.037
Toseland 3.834
ProMoST 4.177
Dawson 4.012
Bjellqvist 4.164
Wikipedia 3.935
Rodwell 3.859
Grimsley 3.745
Solomon 4.012
Lehninger 3.961
Nozaki 4.126
DTASelect 4.342
Thurlkill 3.872
EMBOSS 3.948
Sillero 4.151
Patrickios 3.401
IPC_peptide 4.012
IPC2_peptide 4.126
IPC2.peptide.svr19 4.07
Protein with the highest isoelectric point:
>tr|A0A2D0W8Y0|A0A2D0W8Y0_9CAUD Putative terminase large subunit OS=Bordetella virus LK3 OX=1926943 GN=LK3_02 PE=4 SV=1
MM1 pKa = 7.38 RR2 pKa = 11.84 TLILILAVLPLLATQAHH19 pKa = 6.36 AEE21 pKa = 4.06 EE22 pKa = 4.49 ARR24 pKa = 11.84 PYY26 pKa = 10.13 SAQEE30 pKa = 3.92 TIRR33 pKa = 11.84 RR34 pKa = 11.84 AQIGTHH40 pKa = 5.44 SPRR43 pKa = 11.84 LAPPVRR49 pKa = 11.84 VAPAPARR56 pKa = 11.84 TARR59 pKa = 11.84 PSRR62 pKa = 11.84 YY63 pKa = 9.43 DD64 pKa = 3.38 RR65 pKa = 11.84 LVCRR69 pKa = 11.84 VDD71 pKa = 3.28 PAKK74 pKa = 10.2 PLHH77 pKa = 5.28 QQIICEE83 pKa = 4.1 KK84 pKa = 10.3 QKK86 pKa = 11.27
Molecular weight: 9.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.319
IPC2_protein 9.736
IPC_protein 10.862
Toseland 10.921
ProMoST 11.038
Dawson 10.994
Bjellqvist 10.818
Wikipedia 11.301
Rodwell 10.979
Grimsley 11.038
Solomon 11.257
Lehninger 11.199
Nozaki 10.921
DTASelect 10.818
Thurlkill 10.921
EMBOSS 11.359
Sillero 10.95
Patrickios 10.76
IPC_peptide 11.257
IPC2_peptide 10.189
IPC2.peptide.svr19 8.738
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
79
0
79
18649
37
939
236.1
26.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.719 ± 0.473
1.013 ± 0.109
6.086 ± 0.208
7.083 ± 0.234
3.561 ± 0.145
7.877 ± 0.201
1.759 ± 0.159
4.51 ± 0.158
4.274 ± 0.284
8.826 ± 0.272
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.14 ± 0.126
3.507 ± 0.153
5.309 ± 0.227
4.048 ± 0.155
7.121 ± 0.281
4.713 ± 0.255
5.507 ± 0.253
6.853 ± 0.27
1.936 ± 0.139
2.815 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here