Cassava virus X

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W5VPI1|A0A1W5VPI1_9VIRU ORF1 protein OS=Cassava virus X OX=1977392 GN=RdRp PE=4 SV=1
MM1 pKa = 7.73CSHH4 pKa = 6.65GLMDD8 pKa = 6.46AILRR12 pKa = 11.84EE13 pKa = 4.38LKK15 pKa = 10.51QSGFVQIRR23 pKa = 11.84NHH25 pKa = 5.62LQNPLVVHH33 pKa = 6.44AVAGAGKK40 pKa = 6.88TTLLNKK46 pKa = 10.06LAACSDD52 pKa = 4.77LIIHH56 pKa = 6.24SAAYY60 pKa = 9.39PSGNSLSGNSVQIHH74 pKa = 5.91NPSVTPDD81 pKa = 3.13ILDD84 pKa = 3.87EE85 pKa = 4.18YY86 pKa = 11.3LLVPDD91 pKa = 4.46YY92 pKa = 10.66KK93 pKa = 10.93ASKK96 pKa = 10.47LLIADD101 pKa = 4.51PLQYY105 pKa = 10.21SAKK108 pKa = 9.8PPLAHH113 pKa = 7.14FIKK116 pKa = 10.19ATTHH120 pKa = 6.69RR121 pKa = 11.84FGQSTCALLRR131 pKa = 11.84SLLKK135 pKa = 10.43IEE137 pKa = 4.37VDD139 pKa = 3.12SDD141 pKa = 3.72RR142 pKa = 11.84PDD144 pKa = 3.17TVVISRR150 pKa = 11.84FFEE153 pKa = 4.44GEE155 pKa = 3.84PEE157 pKa = 3.94GAIIAFGEE165 pKa = 4.15EE166 pKa = 4.38AYY168 pKa = 10.66HH169 pKa = 7.14LISAHH174 pKa = 4.59QLKK177 pKa = 10.03PFRR180 pKa = 11.84PCEE183 pKa = 4.01VYY185 pKa = 10.54GLSFPVVTVAFEE197 pKa = 4.19QEE199 pKa = 3.58VDD201 pKa = 3.31KK202 pKa = 11.44YY203 pKa = 10.34PPHH206 pKa = 6.66LVYY209 pKa = 10.71LALSRR214 pKa = 11.84HH215 pKa = 4.63TEE217 pKa = 3.76KK218 pKa = 11.18LIILSDD224 pKa = 3.77ALASSSS230 pKa = 3.33

Molecular weight: 25.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W5VPI7|A0A1W5VPI7_9VIRU Coat protein OS=Cassava virus X OX=1977392 GN=CP PE=3 SV=1
MM1 pKa = 7.88PLQAPPDD8 pKa = 4.06PNRR11 pKa = 11.84TYY13 pKa = 11.13QLVGVCTAVICICYY27 pKa = 10.12FLTQDD32 pKa = 3.1NRR34 pKa = 11.84GFSGDD39 pKa = 3.88RR40 pKa = 11.84EE41 pKa = 3.95NSFPNGGKK49 pKa = 10.28LSYY52 pKa = 10.73CKK54 pKa = 9.34TAVFHH59 pKa = 6.63PPNHH63 pKa = 7.09RR64 pKa = 11.84DD65 pKa = 3.4SQAGAHH71 pKa = 6.41IFLLVIALTALIIFLSRR88 pKa = 11.84RR89 pKa = 11.84TPHH92 pKa = 6.49SCPQCRR98 pKa = 11.84GG99 pKa = 3.13

Molecular weight: 10.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1847

99

1304

461.8

51.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.825 ± 1.751

1.516 ± 0.556

5.36 ± 0.676

5.522 ± 0.743

4.331 ± 0.176

4.656 ± 0.271

2.978 ± 0.412

5.901 ± 0.22

5.685 ± 0.72

10.07 ± 0.538

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.057 ± 0.283

3.736 ± 0.208

6.605 ± 0.198

4.115 ± 0.11

5.035 ± 0.399

6.876 ± 0.553

6.876 ± 0.716

5.631 ± 0.28

1.029 ± 0.246

3.194 ± 0.492
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski