Streptococcus phage SW6
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S7W8C5|A0A3S7W8C5_9CAUD Uncharacterized protein OS=Streptococcus phage SW6 OX=2419655 GN=SW6_034 PE=4 SV=1
MM1 pKa = 7.12 VNWVDD6 pKa = 4.58 KK7 pKa = 11.1 DD8 pKa = 4.24 GNDD11 pKa = 3.97 LPDD14 pKa = 4.26 GADD17 pKa = 3.29 QEE19 pKa = 5.13 FKK21 pKa = 11.28 AGMFFSFAGDD31 pKa = 3.45 EE32 pKa = 4.32 VNITDD37 pKa = 3.77 TGEE40 pKa = 3.65 GGYY43 pKa = 10.61 YY44 pKa = 9.76 GGYY47 pKa = 8.0 YY48 pKa = 9.41 YY49 pKa = 10.76 RR50 pKa = 11.84 RR51 pKa = 11.84 FEE53 pKa = 4.06 FGQFGTVWLSCWNKK67 pKa = 10.66 DD68 pKa = 3.89 DD69 pKa = 5.14 LVNYY73 pKa = 7.38 YY74 pKa = 8.26 QQ75 pKa = 4.22
Molecular weight: 8.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.798
IPC2_protein 3.897
IPC_protein 3.834
Toseland 3.617
ProMoST 4.024
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.808
Rodwell 3.656
Grimsley 3.528
Solomon 3.808
Lehninger 3.77
Nozaki 3.961
DTASelect 4.202
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.948
Patrickios 0.477
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.851
Protein with the highest isoelectric point:
>tr|A0A3S5H0K5|A0A3S5H0K5_9CAUD Uncharacterized protein OS=Streptococcus phage SW6 OX=2419655 GN=SW6_041 PE=4 SV=1
MM1 pKa = 6.65 TTLSKK6 pKa = 10.54 RR7 pKa = 11.84 DD8 pKa = 3.43 NQLRR12 pKa = 11.84 ILTAVINVTTIIRR25 pKa = 11.84 VVNRR29 pKa = 11.84 PIEE32 pKa = 4.27 TVVIQAII39 pKa = 3.69
Molecular weight: 4.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.161
IPC2_protein 9.853
IPC_protein 11.374
Toseland 11.564
ProMoST 12.032
Dawson 11.564
Bjellqvist 11.535
Wikipedia 12.018
Rodwell 11.33
Grimsley 11.608
Solomon 12.047
Lehninger 11.945
Nozaki 11.564
DTASelect 11.535
Thurlkill 11.564
EMBOSS 12.047
Sillero 11.564
Patrickios 11.199
IPC_peptide 12.047
IPC2_peptide 11.023
IPC2.peptide.svr19 9.579
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
42
0
42
10062
39
1594
239.6
27.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.539 ± 0.525
0.477 ± 0.131
6.639 ± 0.234
6.44 ± 0.369
4.124 ± 0.218
6.698 ± 0.49
1.431 ± 0.107
6.619 ± 0.226
8.289 ± 0.496
7.901 ± 0.39
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.405 ± 0.164
6.48 ± 0.299
3.071 ± 0.177
4.184 ± 0.18
4.154 ± 0.322
6.47 ± 0.339
6.539 ± 0.29
6.092 ± 0.326
1.481 ± 0.175
3.965 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here