Avian HDV-like agent
Average proteome isoelectric point is 8.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A386CB41|A0A386CB41_9VIRU HDAg-like protein OS=Avian HDV-like agent OX=2364132 PE=3 SV=1
MM1 pKa = 7.4 EE2 pKa = 5.31 NKK4 pKa = 10.24 EE5 pKa = 3.83 EE6 pKa = 4.13 SQKK9 pKa = 10.88 KK10 pKa = 9.09 KK11 pKa = 10.34 RR12 pKa = 11.84 GRR14 pKa = 11.84 EE15 pKa = 3.59 EE16 pKa = 4.1 TLQKK20 pKa = 10.26 WVDD23 pKa = 3.09 DD24 pKa = 4.17 RR25 pKa = 11.84 KK26 pKa = 10.5 RR27 pKa = 11.84 KK28 pKa = 9.91 RR29 pKa = 11.84 EE30 pKa = 4.02 LEE32 pKa = 4.04 EE33 pKa = 3.79 EE34 pKa = 4.22 LEE36 pKa = 4.21 KK37 pKa = 10.81 LRR39 pKa = 11.84 KK40 pKa = 9.46 RR41 pKa = 11.84 IKK43 pKa = 10.42 DD44 pKa = 3.63 RR45 pKa = 11.84 EE46 pKa = 4.2 RR47 pKa = 11.84 KK48 pKa = 9.85 NPWLGNLKK56 pKa = 10.49 GMLKK60 pKa = 9.77 QDD62 pKa = 3.27 SGGGQEE68 pKa = 4.41 KK69 pKa = 10.24 KK70 pKa = 10.48 KK71 pKa = 10.44 EE72 pKa = 3.91 AAEE75 pKa = 4.06 PMDD78 pKa = 3.76 TGEE81 pKa = 4.55 SPKK84 pKa = 10.48 KK85 pKa = 10.07 KK86 pKa = 9.84 KK87 pKa = 10.5 KK88 pKa = 9.87 KK89 pKa = 10.15 DD90 pKa = 3.49 YY91 pKa = 10.04 EE92 pKa = 4.4 GLSFTPEE99 pKa = 3.19 EE100 pKa = 4.23 KK101 pKa = 9.88 QRR103 pKa = 11.84 HH104 pKa = 4.62 KK105 pKa = 10.76 RR106 pKa = 11.84 KK107 pKa = 9.8 CDD109 pKa = 3.46 LEE111 pKa = 4.21 NKK113 pKa = 9.6 KK114 pKa = 10.4 KK115 pKa = 10.27 QLNAKK120 pKa = 9.14 GKK122 pKa = 9.31 QLTSQEE128 pKa = 3.78 EE129 pKa = 4.26 DD130 pKa = 3.07 EE131 pKa = 4.73 LRR133 pKa = 11.84 NLQEE137 pKa = 3.89 EE138 pKa = 4.35 DD139 pKa = 3.54 KK140 pKa = 11.29 KK141 pKa = 11.21 RR142 pKa = 11.84 LLKK145 pKa = 10.48 KK146 pKa = 9.86 RR147 pKa = 11.84 EE148 pKa = 3.73 EE149 pKa = 4.05 RR150 pKa = 11.84 EE151 pKa = 3.88 RR152 pKa = 11.84 RR153 pKa = 11.84 SGGVNLFGSASPSTSGGGNASSTQGLRR180 pKa = 11.84 LPWQKK185 pKa = 11.35
Molecular weight: 21.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.914
IPC2_protein 9.063
IPC_protein 8.96
Toseland 10.262
ProMoST 9.692
Dawson 10.321
Bjellqvist 9.853
Wikipedia 10.379
Rodwell 11.052
Grimsley 10.335
Solomon 10.35
Lehninger 10.335
Nozaki 10.233
DTASelect 9.853
Thurlkill 10.218
EMBOSS 10.628
Sillero 10.233
Patrickios 10.789
IPC_peptide 10.365
IPC2_peptide 8.112
IPC2.peptide.svr19 8.082
Protein with the highest isoelectric point:
>tr|A0A386CB41|A0A386CB41_9VIRU HDAg-like protein OS=Avian HDV-like agent OX=2364132 PE=3 SV=1
MM1 pKa = 7.4 EE2 pKa = 5.31 NKK4 pKa = 10.24 EE5 pKa = 3.83 EE6 pKa = 4.13 SQKK9 pKa = 10.88 KK10 pKa = 9.09 KK11 pKa = 10.34 RR12 pKa = 11.84 GRR14 pKa = 11.84 EE15 pKa = 3.59 EE16 pKa = 4.1 TLQKK20 pKa = 10.26 WVDD23 pKa = 3.09 DD24 pKa = 4.17 RR25 pKa = 11.84 KK26 pKa = 10.5 RR27 pKa = 11.84 KK28 pKa = 9.91 RR29 pKa = 11.84 EE30 pKa = 4.02 LEE32 pKa = 4.04 EE33 pKa = 3.79 EE34 pKa = 4.22 LEE36 pKa = 4.21 KK37 pKa = 10.81 LRR39 pKa = 11.84 KK40 pKa = 9.46 RR41 pKa = 11.84 IKK43 pKa = 10.42 DD44 pKa = 3.63 RR45 pKa = 11.84 EE46 pKa = 4.2 RR47 pKa = 11.84 KK48 pKa = 9.85 NPWLGNLKK56 pKa = 10.49 GMLKK60 pKa = 9.77 QDD62 pKa = 3.27 SGGGQEE68 pKa = 4.41 KK69 pKa = 10.24 KK70 pKa = 10.48 KK71 pKa = 10.44 EE72 pKa = 3.91 AAEE75 pKa = 4.06 PMDD78 pKa = 3.76 TGEE81 pKa = 4.55 SPKK84 pKa = 10.48 KK85 pKa = 10.07 KK86 pKa = 9.84 KK87 pKa = 10.5 KK88 pKa = 9.87 KK89 pKa = 10.15 DD90 pKa = 3.49 YY91 pKa = 10.04 EE92 pKa = 4.4 GLSFTPEE99 pKa = 3.19 EE100 pKa = 4.23 KK101 pKa = 9.88 QRR103 pKa = 11.84 HH104 pKa = 4.62 KK105 pKa = 10.76 RR106 pKa = 11.84 KK107 pKa = 9.8 CDD109 pKa = 3.46 LEE111 pKa = 4.21 NKK113 pKa = 9.6 KK114 pKa = 10.4 KK115 pKa = 10.27 QLNAKK120 pKa = 9.14 GKK122 pKa = 9.31 QLTSQEE128 pKa = 3.78 EE129 pKa = 4.26 DD130 pKa = 3.07 EE131 pKa = 4.73 LRR133 pKa = 11.84 NLQEE137 pKa = 3.89 EE138 pKa = 4.35 DD139 pKa = 3.54 KK140 pKa = 11.29 KK141 pKa = 11.21 RR142 pKa = 11.84 LLKK145 pKa = 10.48 KK146 pKa = 9.86 RR147 pKa = 11.84 EE148 pKa = 3.73 EE149 pKa = 4.05 RR150 pKa = 11.84 EE151 pKa = 3.88 RR152 pKa = 11.84 RR153 pKa = 11.84 SGGVNLFGSASPSTSGGGNASSTQGLRR180 pKa = 11.84 LPWQKK185 pKa = 11.35
Molecular weight: 21.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.914
IPC2_protein 9.063
IPC_protein 8.96
Toseland 10.262
ProMoST 9.692
Dawson 10.321
Bjellqvist 9.853
Wikipedia 10.379
Rodwell 11.052
Grimsley 10.335
Solomon 10.35
Lehninger 10.335
Nozaki 10.233
DTASelect 9.853
Thurlkill 10.218
EMBOSS 10.628
Sillero 10.233
Patrickios 10.789
IPC_peptide 10.365
IPC2_peptide 8.112
IPC2.peptide.svr19 8.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
185
185
185
185.0
21.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.703 ± 0.0
0.541 ± 0.0
4.865 ± 0.0
14.595 ± 0.0
1.081 ± 0.0
8.649 ± 0.0
0.541 ± 0.0
0.541 ± 0.0
18.919 ± 0.0
9.73 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.622 ± 0.0
4.324 ± 0.0
3.243 ± 0.0
5.946 ± 0.0
9.73 ± 0.0
6.486 ± 0.0
3.243 ± 0.0
1.081 ± 0.0
1.622 ± 0.0
0.541 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here