Streptomyces sp. HPH0547

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6620 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S3BA85|S3BA85_9ACTN Orotate phosphoribosyltransferase OS=Streptomyces sp. HPH0547 OX=1203592 GN=pyrE PE=3 SV=1
MM1 pKa = 7.07TAQDD5 pKa = 3.43EE6 pKa = 4.6SLDD9 pKa = 3.52VWIDD13 pKa = 3.48QEE15 pKa = 4.69LCTGDD20 pKa = 4.89GICSQYY26 pKa = 11.39APDD29 pKa = 4.36VFEE32 pKa = 6.18LDD34 pKa = 3.22IDD36 pKa = 3.59GLAYY40 pKa = 10.69VKK42 pKa = 10.77GADD45 pKa = 3.36GEE47 pKa = 4.28LRR49 pKa = 11.84QEE51 pKa = 3.98EE52 pKa = 4.89GAAVPVPADD61 pKa = 3.38AVRR64 pKa = 11.84DD65 pKa = 3.92VVDD68 pKa = 4.25SVKK71 pKa = 10.68DD72 pKa = 3.68CPGDD76 pKa = 4.08CIHH79 pKa = 6.34VRR81 pKa = 11.84RR82 pKa = 11.84VSDD85 pKa = 3.56GVEE88 pKa = 4.09VYY90 pKa = 10.78GPEE93 pKa = 4.93AVDD96 pKa = 3.7TPP98 pKa = 4.07

Molecular weight: 10.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S3BRJ5|S3BRJ5_9ACTN Peptide deformylase OS=Streptomyces sp. HPH0547 OX=1203592 GN=def PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.82GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6620

0

6620

2249410

28

23217

339.8

36.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.068 ± 0.047

0.832 ± 0.011

5.78 ± 0.022

5.893 ± 0.034

2.562 ± 0.019

9.981 ± 0.036

2.411 ± 0.016

2.64 ± 0.027

2.006 ± 0.028

10.184 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.68 ± 0.013

1.551 ± 0.015

6.604 ± 0.033

2.748 ± 0.025

8.644 ± 0.038

4.83 ± 0.024

6.043 ± 0.027

8.169 ± 0.029

1.467 ± 0.013

1.908 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski