Bacteroides sp. CAG:770
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2085 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R6T5L5|R6T5L5_9BACE Antioxidant AhpC/TSA family OS=Bacteroides sp. CAG:770 OX=1262751 GN=BN777_01200 PE=4 SV=1
MM1 pKa = 7.36 GNDD4 pKa = 3.21 MNFGNKK10 pKa = 7.99 MPYY13 pKa = 9.6 NVPEE17 pKa = 4.86 GYY19 pKa = 10.45 FEE21 pKa = 4.22 GLRR24 pKa = 11.84 AKK26 pKa = 10.29 ISEE29 pKa = 4.17 IPDD32 pKa = 3.41 RR33 pKa = 11.84 EE34 pKa = 4.25 PEE36 pKa = 3.87 VTLISRR42 pKa = 11.84 VRR44 pKa = 11.84 PYY46 pKa = 10.38 FALAAAFVAIVVCGTAVLKK65 pKa = 9.45 ITSGVSEE72 pKa = 4.41 VEE74 pKa = 4.07 EE75 pKa = 4.1 MSALDD80 pKa = 3.9 KK81 pKa = 10.83 MQMADD86 pKa = 3.43 IVPVTDD92 pKa = 4.89 PYY94 pKa = 11.37 SIYY97 pKa = 10.69 CGLQEE102 pKa = 5.13 EE103 pKa = 6.02 GITHH107 pKa = 7.53 DD108 pKa = 5.96 DD109 pKa = 2.95 ITAYY113 pKa = 10.86 LIDD116 pKa = 4.02 SGTTLEE122 pKa = 4.55 HH123 pKa = 6.17 IEE125 pKa = 4.41 YY126 pKa = 10.68 YY127 pKa = 10.63 DD128 pKa = 4.94 AGNDD132 pKa = 3.58 NQQ134 pKa = 3.94
Molecular weight: 14.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.88
IPC2_protein 4.151
IPC_protein 4.075
Toseland 3.897
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.935
Rodwell 3.91
Grimsley 3.808
Solomon 4.037
Lehninger 3.986
Nozaki 4.164
DTASelect 4.329
Thurlkill 3.935
EMBOSS 3.948
Sillero 4.19
Patrickios 1.036
IPC_peptide 4.037
IPC2_peptide 4.177
IPC2.peptide.svr19 4.083
Protein with the highest isoelectric point:
>tr|R6SYP5|R6SYP5_9BACE 10 kDa chaperonin OS=Bacteroides sp. CAG:770 OX=1262751 GN=groS PE=3 SV=1
MM1 pKa = 7.84 PSGKK5 pKa = 9.39 KK6 pKa = 9.62 RR7 pKa = 11.84 KK8 pKa = 6.72 RR9 pKa = 11.84 HH10 pKa = 5.17 KK11 pKa = 10.05 MATHH15 pKa = 6.02 KK16 pKa = 10.34 RR17 pKa = 11.84 KK18 pKa = 9.84 KK19 pKa = 9.28 RR20 pKa = 11.84 LRR22 pKa = 11.84 KK23 pKa = 9.26 NRR25 pKa = 11.84 HH26 pKa = 4.71 KK27 pKa = 10.87 KK28 pKa = 9.36 KK29 pKa = 10.77
Molecular weight: 3.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.023
IPC_protein 12.544
Toseland 12.735
ProMoST 13.203
Dawson 12.735
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.705
Grimsley 12.764
Solomon 13.217
Lehninger 13.115
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.427
IPC_peptide 13.217
IPC2_peptide 12.193
IPC2.peptide.svr19 8.989
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2085
0
2085
776372
29
1866
372.4
41.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.902 ± 0.045
1.324 ± 0.022
6.135 ± 0.035
6.274 ± 0.051
4.489 ± 0.032
7.385 ± 0.039
1.678 ± 0.021
6.479 ± 0.043
6.398 ± 0.036
8.375 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.836 ± 0.023
4.638 ± 0.037
3.803 ± 0.027
2.713 ± 0.026
4.709 ± 0.042
6.929 ± 0.048
5.497 ± 0.04
6.955 ± 0.043
1.268 ± 0.021
4.21 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here