African swine fever virus (ASFV)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; Asfuvirales; Asfarviridae; Asfivirus

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 163 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A1DXW6|A0A0A1DXW6_ASF PI9R OS=African swine fever virus OX=10497 GN=I9R PE=4 SV=1
MM1 pKa = 7.46VSRR4 pKa = 11.84FLIAEE9 pKa = 4.0YY10 pKa = 10.43RR11 pKa = 11.84HH12 pKa = 6.45LIEE15 pKa = 5.41NPSEE19 pKa = 3.97NFKK22 pKa = 10.92ISVNEE27 pKa = 3.91NNITEE32 pKa = 4.12WDD34 pKa = 3.9VILRR38 pKa = 11.84GPPDD42 pKa = 3.39TLYY45 pKa = 11.18EE46 pKa = 4.56GGLFKK51 pKa = 11.06AKK53 pKa = 10.14VAFPPEE59 pKa = 4.17YY60 pKa = 9.88PYY62 pKa = 11.3APPKK66 pKa = 9.97LTFTSEE72 pKa = 3.38MWHH75 pKa = 6.31PNIYY79 pKa = 9.88PDD81 pKa = 3.75GRR83 pKa = 11.84LCISILHH90 pKa = 6.64GDD92 pKa = 3.56NAEE95 pKa = 4.12EE96 pKa = 5.24QGMTWSPAQKK106 pKa = 9.64IDD108 pKa = 3.88TILLSVISLLNEE120 pKa = 4.11PNPDD124 pKa = 3.04SPANVDD130 pKa = 3.14AAKK133 pKa = 10.28SYY135 pKa = 10.83RR136 pKa = 11.84KK137 pKa = 9.61YY138 pKa = 10.9VYY140 pKa = 10.51KK141 pKa = 10.39EE142 pKa = 3.95DD143 pKa = 3.96LEE145 pKa = 4.52SYY147 pKa = 10.2PMEE150 pKa = 4.05VKK152 pKa = 9.46KK153 pKa = 8.91TVKK156 pKa = 10.25KK157 pKa = 10.92SLDD160 pKa = 3.35EE161 pKa = 4.43CSPEE165 pKa = 4.36DD166 pKa = 3.84IEE168 pKa = 4.43YY169 pKa = 10.53FKK171 pKa = 11.24NAASNVPPIPSDD183 pKa = 3.31AYY185 pKa = 10.13EE186 pKa = 4.47DD187 pKa = 3.69EE188 pKa = 4.66CEE190 pKa = 4.09EE191 pKa = 4.45MEE193 pKa = 5.26DD194 pKa = 3.27DD195 pKa = 4.73TYY197 pKa = 11.61ILTYY201 pKa = 11.0DD202 pKa = 4.06DD203 pKa = 5.65DD204 pKa = 4.52EE205 pKa = 4.86EE206 pKa = 5.45EE207 pKa = 4.44EE208 pKa = 4.36DD209 pKa = 5.05EE210 pKa = 5.3EE211 pKa = 4.71MDD213 pKa = 5.41DD214 pKa = 4.1EE215 pKa = 4.56

Molecular weight: 24.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A1DXP6|A0A0A1DXP6_ASF Uncharacterized protein B175L OS=African swine fever virus OX=10497 GN=B175L PE=3 SV=1
MM1 pKa = 7.22NWGSISSGTPGLFVEE16 pKa = 5.55SIRR19 pKa = 11.84NTPSVVKK26 pKa = 10.59INVIFLKK33 pKa = 10.64VISNTAVSVFWRR45 pKa = 11.84DD46 pKa = 2.66RR47 pKa = 11.84RR48 pKa = 11.84IRR50 pKa = 11.84FEE52 pKa = 4.32SDD54 pKa = 2.11WLNSYY59 pKa = 7.17FQKK62 pKa = 11.15

Molecular weight: 7.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

163

0

163

54376

60

2475

333.6

38.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.195 ± 0.139

2.084 ± 0.125

4.605 ± 0.1

6.247 ± 0.142

4.813 ± 0.113

4.302 ± 0.151

2.935 ± 0.122

8.395 ± 0.144

7.483 ± 0.209

10.354 ± 0.178

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.668 ± 0.074

6.023 ± 0.127

4.206 ± 0.163

4.188 ± 0.128

4.029 ± 0.153

6.034 ± 0.135

5.536 ± 0.128

5.068 ± 0.12

1.096 ± 0.059

4.739 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski