Acheta domesticus volvovirus
Average proteome isoelectric point is 8.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M9TDU6|M9TDU6_9VIRU Uncharacterized protein OS=Acheta domesticus volvovirus OX=1291515 PE=4 SV=1
MM1 pKa = 8.17 RR2 pKa = 11.84 FRR4 pKa = 11.84 HH5 pKa = 5.89 WIGTSFNLEE14 pKa = 4.4 SIPDD18 pKa = 3.87 DD19 pKa = 3.66 EE20 pKa = 4.71 STIKK24 pKa = 10.55 QIAYY28 pKa = 9.25 QRR30 pKa = 11.84 EE31 pKa = 4.13 RR32 pKa = 11.84 CPEE35 pKa = 3.89 TNRR38 pKa = 11.84 LHH40 pKa = 6.64 LQFCISFTNPRR51 pKa = 11.84 TMDD54 pKa = 3.12 GVKK57 pKa = 9.97 RR58 pKa = 11.84 YY59 pKa = 10.08 IGDD62 pKa = 3.37 QSAHH66 pKa = 6.73 LEE68 pKa = 3.89 PCRR71 pKa = 11.84 NLRR74 pKa = 11.84 KK75 pKa = 10.06 GLEE78 pKa = 4.07 YY79 pKa = 10.75 CNKK82 pKa = 10.09 SEE84 pKa = 4.53 SFVDD88 pKa = 3.4 NRR90 pKa = 11.84 FSRR93 pKa = 11.84 FNTDD97 pKa = 2.85 AQDD100 pKa = 3.69 APDD103 pKa = 4.14 DD104 pKa = 3.9 FRR106 pKa = 11.84 EE107 pKa = 4.26 FTEE110 pKa = 4.05 LQLWQKK116 pKa = 9.49 YY117 pKa = 7.23 PNWMLKK123 pKa = 10.13 HH124 pKa = 5.18 GTQVRR129 pKa = 11.84 RR130 pKa = 11.84 YY131 pKa = 8.23 YY132 pKa = 9.92 QICQEE137 pKa = 4.19 VPQTRR142 pKa = 11.84 EE143 pKa = 3.65 KK144 pKa = 9.58 PVCYY148 pKa = 10.02 IFWGPAGTGKK158 pKa = 10.38 SYY160 pKa = 10.84 SARR163 pKa = 11.84 HH164 pKa = 4.73 WLGDD168 pKa = 3.31 QLYY171 pKa = 9.31 IKK173 pKa = 10.22 PPGNFWIGYY182 pKa = 7.98 NGEE185 pKa = 4.16 KK186 pKa = 10.45 AVLFDD191 pKa = 5.52 DD192 pKa = 4.88 YY193 pKa = 11.53 YY194 pKa = 11.67 SSEE197 pKa = 4.55 KK198 pKa = 10.73 YY199 pKa = 10.45 DD200 pKa = 4.92 DD201 pKa = 3.86 LLRR204 pKa = 11.84 WISEE208 pKa = 4.15 NPIHH212 pKa = 7.27 VSIKK216 pKa = 10.55 GSSTPLKK223 pKa = 10.1 AIKK226 pKa = 9.82 FAFTSNMNPRR236 pKa = 11.84 SWHH239 pKa = 5.88 SKK241 pKa = 10.1 IEE243 pKa = 3.95 DD244 pKa = 3.16 HH245 pKa = 6.79 SALFRR250 pKa = 11.84 RR251 pKa = 11.84 ITKK254 pKa = 10.26 CFFCTDD260 pKa = 2.95 KK261 pKa = 11.23 CFSLDD266 pKa = 3.7 NLHH269 pKa = 6.6 EE270 pKa = 4.26
Molecular weight: 32.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.255
IPC2_protein 7.541
IPC_protein 7.41
Toseland 7.044
ProMoST 7.922
Dawson 8.097
Bjellqvist 8.492
Wikipedia 8.009
Rodwell 8.112
Grimsley 7.102
Solomon 8.17
Lehninger 8.185
Nozaki 8.712
DTASelect 8.214
Thurlkill 8.258
EMBOSS 8.317
Sillero 8.595
Patrickios 4.151
IPC_peptide 8.156
IPC2_peptide 7.614
IPC2.peptide.svr19 7.6
Protein with the highest isoelectric point:
>tr|M9THR5|M9THR5_9VIRU Uncharacterized protein OS=Acheta domesticus volvovirus OX=1291515 PE=4 SV=1
MM1 pKa = 7.39 ARR3 pKa = 11.84 LVKK6 pKa = 10.34 SSRR9 pKa = 11.84 RR10 pKa = 11.84 LMHH13 pKa = 6.12 RR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 GFAIRR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 YY27 pKa = 8.24 SGRR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 YY33 pKa = 7.58 STRR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 YY39 pKa = 9.8 SIRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 VSRR47 pKa = 11.84 SLAIPRR53 pKa = 11.84 RR54 pKa = 11.84 GARR57 pKa = 11.84 EE58 pKa = 3.79 FSWVLNLHH66 pKa = 6.64 NYY68 pKa = 7.06 QHH70 pKa = 6.46 SFNDD74 pKa = 3.52 GGTISAIQGNQWYY87 pKa = 10.21 LMSPPTLIIPYY98 pKa = 9.12 QAFRR102 pKa = 11.84 FLFKK106 pKa = 10.14 EE107 pKa = 3.73 WRR109 pKa = 11.84 LTRR112 pKa = 11.84 LLVAIRR118 pKa = 11.84 RR119 pKa = 11.84 RR120 pKa = 11.84 YY121 pKa = 8.65 PEE123 pKa = 3.84 PVVYY127 pKa = 10.34 QPNQQEE133 pKa = 4.08 QTVAASRR140 pKa = 11.84 LASNNIWWCPWGKK153 pKa = 10.26 YY154 pKa = 9.25 DD155 pKa = 5.83 RR156 pKa = 11.84 PEE158 pKa = 3.66 QAPRR162 pKa = 11.84 QTKK165 pKa = 10.02 SAILLTSQWAVRR177 pKa = 11.84 SVPQRR182 pKa = 11.84 CQRR185 pKa = 11.84 LSLVSTKK192 pKa = 10.42 RR193 pKa = 11.84 SGSFVSGSTPIIDD206 pKa = 3.87 GNEE209 pKa = 3.6 GRR211 pKa = 11.84 PGGGYY216 pKa = 10.07 GQGALLSNVGFVAYY230 pKa = 9.27 WGRR233 pKa = 11.84 WPTYY237 pKa = 10.45 SFSDD241 pKa = 3.77 TYY243 pKa = 11.51 NAIANSDD250 pKa = 3.94 RR251 pKa = 11.84 NDD253 pKa = 4.1 LLPAANLGFIAVEE266 pKa = 4.05 NLSEE270 pKa = 4.07 ITPDD274 pKa = 3.16 AVEE277 pKa = 4.22 IKK279 pKa = 10.62 VKK281 pKa = 10.88 AFFSFKK287 pKa = 10.23 GRR289 pKa = 11.84 KK290 pKa = 8.57 NLGSVNDD297 pKa = 3.65 STGAFTSAFPLSSTDD312 pKa = 2.92 WYY314 pKa = 11.07 LDD316 pKa = 3.38 NNKK319 pKa = 8.41 TVPPVGVLEE328 pKa = 4.3 QNLLDD333 pKa = 4.6 QIDD336 pKa = 3.86 KK337 pKa = 10.94 NEE339 pKa = 4.06 PVEE342 pKa = 4.35 MPDD345 pKa = 3.16 IDD347 pKa = 4.08 PASVVAASPDD357 pKa = 3.33 GPMVV361 pKa = 3.37
Molecular weight: 41.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.327
IPC2_protein 9.911
IPC_protein 10.921
Toseland 10.774
ProMoST 10.745
Dawson 10.891
Bjellqvist 10.716
Wikipedia 11.199
Rodwell 10.906
Grimsley 10.965
Solomon 11.096
Lehninger 11.038
Nozaki 10.76
DTASelect 10.716
Thurlkill 10.804
EMBOSS 11.213
Sillero 10.847
Patrickios 10.555
IPC_peptide 11.096
IPC2_peptide 9.882
IPC2.peptide.svr19 8.382
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
968
130
361
242.0
27.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.165 ± 0.988
1.446 ± 0.69
4.855 ± 0.53
4.442 ± 0.628
4.442 ± 0.924
6.095 ± 0.767
1.756 ± 0.6
4.752 ± 0.514
5.062 ± 0.855
6.095 ± 0.897
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.446 ± 0.132
5.579 ± 0.554
6.612 ± 0.692
4.545 ± 0.312
9.504 ± 1.168
9.711 ± 1.082
7.438 ± 1.904
4.339 ± 1.064
2.583 ± 0.364
4.132 ± 0.275
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here