Jatropha leaf curl virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5A470|B5A470_9GEMI Replication-associated protein OS=Jatropha leaf curl virus OX=543876 PE=3 SV=1
MM1 pKa = 7.27SCSTATVPLQLLSRR15 pKa = 11.84DD16 pKa = 3.43ARR18 pKa = 11.84GDD20 pKa = 3.21RR21 pKa = 11.84SGGDD25 pKa = 3.0KK26 pKa = 11.19AEE28 pKa = 4.15EE29 pKa = 4.03DD30 pKa = 3.58RR31 pKa = 11.84LEE33 pKa = 3.7VRR35 pKa = 11.84MFIFSLANCANHH47 pKa = 6.76GFTHH51 pKa = 7.39RR52 pKa = 11.84GTHH55 pKa = 5.84HH56 pKa = 6.91CSSFQQWRR64 pKa = 11.84LYY66 pKa = 11.05LDD68 pKa = 3.99GPKK71 pKa = 10.36SSVLPRR77 pKa = 11.84HH78 pKa = 5.61QSHH81 pKa = 6.15EE82 pKa = 4.15QTLQPGPGHH91 pKa = 5.98HH92 pKa = 6.79TPPNTIQSQSQASTTDD108 pKa = 3.42TQVLPEE114 pKa = 4.35LQSLDD119 pKa = 3.88SFTDD123 pKa = 3.39SDD125 pKa = 3.86WDD127 pKa = 3.76VLTSLL132 pKa = 4.93

Molecular weight: 14.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5A469|B5A469_9GEMI Transcriptional activator protein OS=Jatropha leaf curl virus OX=543876 PE=3 SV=1
MM1 pKa = 8.22DD2 pKa = 4.53SRR4 pKa = 11.84TGEE7 pKa = 4.61PITAARR13 pKa = 11.84FNNGVFIWTVPNPLYY28 pKa = 10.73FRR30 pKa = 11.84VISHH34 pKa = 6.51MSRR37 pKa = 11.84PFNLDD42 pKa = 2.36QDD44 pKa = 4.43IIHH47 pKa = 6.83LRR49 pKa = 11.84IQFNHH54 pKa = 6.34NLRR57 pKa = 11.84QALQIHH63 pKa = 6.24KK64 pKa = 9.72CFLSFKK70 pKa = 8.64VWTRR74 pKa = 11.84SRR76 pKa = 11.84IQTGMFLRR84 pKa = 11.84VFKK87 pKa = 9.72TQVIRR92 pKa = 11.84YY93 pKa = 8.39LDD95 pKa = 3.66RR96 pKa = 11.84LGVISINLVIKK107 pKa = 10.62AVDD110 pKa = 3.31HH111 pKa = 5.44VLYY114 pKa = 10.79NVLHH118 pKa = 6.26HH119 pKa = 6.43TMQVEE124 pKa = 3.96QSNEE128 pKa = 3.28IMFNN132 pKa = 3.38

Molecular weight: 15.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1072

79

354

178.7

20.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.69 ± 0.939

1.772 ± 0.394

5.597 ± 0.621

4.384 ± 0.688

4.384 ± 0.704

5.131 ± 0.549

3.825 ± 0.61

5.877 ± 1.035

4.944 ± 1.08

7.929 ± 0.958

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.425 ± 0.799

4.851 ± 0.657

6.063 ± 0.634

4.104 ± 0.932

8.022 ± 0.724

9.049 ± 1.423

4.664 ± 1.098

6.343 ± 0.725

1.586 ± 0.288

3.358 ± 0.727
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski