Silicimonas algicola
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4315 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A316G8Z5|A0A316G8Z5_9RHOB Glutamate 5-kinase OS=Silicimonas algicola OX=1826607 GN=proB PE=3 SV=1
MM1 pKa = 6.96 TCKK4 pKa = 10.58 ALIPGLLALATLSGCSGGGGGAVYY28 pKa = 10.94 ADD30 pKa = 4.14 GPSPASIDD38 pKa = 4.03 EE39 pKa = 4.23 IVGLQFPLRR48 pKa = 11.84 SARR51 pKa = 11.84 LTSDD55 pKa = 4.85 DD56 pKa = 4.52 GFTTANAGLSTYY68 pKa = 9.87 TIEE71 pKa = 4.34 FTSGTTANLTTPAGTVEE88 pKa = 4.12 LTDD91 pKa = 3.65 GGGMNFTGSLNGSDD105 pKa = 4.24 YY106 pKa = 11.15 FLTSLGWGHH115 pKa = 7.39 DD116 pKa = 3.64 FLEE119 pKa = 4.69 GLGLEE124 pKa = 4.63 VTDD127 pKa = 3.93 GMGNVLVATGVFGLEE142 pKa = 4.01 TRR144 pKa = 11.84 PEE146 pKa = 4.4 DD147 pKa = 3.62 VPEE150 pKa = 4.22 AAVVANFLGGSEE162 pKa = 4.08 LHH164 pKa = 6.05 GTVNDD169 pKa = 3.5 VPFFDD174 pKa = 3.86 TGAANLTADD183 pKa = 5.04 FGAQTVGGNVFVGGGTTLAFDD204 pKa = 4.24 GPVPIVGNTFSGDD217 pKa = 3.59 LEE219 pKa = 4.45 IFSATATLNEE229 pKa = 4.1 GTATGRR235 pKa = 11.84 FYY237 pKa = 11.68 GDD239 pKa = 3.12 AAQVVAGTYY248 pKa = 10.08 SGSATEE254 pKa = 4.82 AGSDD258 pKa = 3.27 VDD260 pKa = 4.04 FAGVFHH266 pKa = 7.6 ADD268 pKa = 2.94
Molecular weight: 26.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.617
IPC_protein 3.617
Toseland 3.401
ProMoST 3.757
Dawson 3.617
Bjellqvist 3.808
Wikipedia 3.567
Rodwell 3.452
Grimsley 3.312
Solomon 3.605
Lehninger 3.554
Nozaki 3.745
DTASelect 3.973
Thurlkill 3.465
EMBOSS 3.579
Sillero 3.745
Patrickios 0.947
IPC_peptide 3.605
IPC2_peptide 3.719
IPC2.peptide.svr19 3.716
Protein with the highest isoelectric point:
>tr|A0A316GD04|A0A316GD04_9RHOB Uncharacterized protein DUF1446 OS=Silicimonas algicola OX=1826607 GN=C8D95_101634 PE=4 SV=1
MM1 pKa = 7.26 LHH3 pKa = 5.01 HH4 pKa = 6.98 HH5 pKa = 6.47 FRR7 pKa = 11.84 RR8 pKa = 11.84 FLDD11 pKa = 4.25 ALDD14 pKa = 5.01 DD15 pKa = 4.48 LNPAQIEE22 pKa = 4.09 DD23 pKa = 4.06 AQTKK27 pKa = 9.83 IRR29 pKa = 11.84 DD30 pKa = 3.36 IRR32 pKa = 11.84 RR33 pKa = 11.84 KK34 pKa = 8.1 TEE36 pKa = 4.47 AISEE40 pKa = 4.06 IEE42 pKa = 3.7 ARR44 pKa = 11.84 TNRR47 pKa = 11.84 EE48 pKa = 4.06 HH49 pKa = 7.25 KK50 pKa = 10.76 CPFCGDD56 pKa = 3.07 EE57 pKa = 5.39 RR58 pKa = 11.84 RR59 pKa = 11.84 QKK61 pKa = 9.63 WGRR64 pKa = 11.84 TRR66 pKa = 11.84 TKK68 pKa = 8.8 IQRR71 pKa = 11.84 YY72 pKa = 8.2 RR73 pKa = 11.84 CSGCRR78 pKa = 11.84 KK79 pKa = 7.68 TYY81 pKa = 10.22 SGRR84 pKa = 11.84 TGSAIGRR91 pKa = 11.84 IHH93 pKa = 7.36 RR94 pKa = 11.84 PDD96 pKa = 3.69 LFLVALRR103 pKa = 11.84 DD104 pKa = 3.82 MLDD107 pKa = 3.03 ASAPQSVRR115 pKa = 11.84 KK116 pKa = 9.05 LARR119 pKa = 11.84 QLDD122 pKa = 4.16 LNKK125 pKa = 9.1 YY126 pKa = 6.29 TVWRR130 pKa = 11.84 WRR132 pKa = 11.84 MIVFSIIGSRR142 pKa = 11.84 SVATSFSGIIEE153 pKa = 4.19 ADD155 pKa = 3.05 EE156 pKa = 4.5 TYY158 pKa = 10.6 QRR160 pKa = 11.84 EE161 pKa = 4.19 SRR163 pKa = 11.84 KK164 pKa = 10.06 GSRR167 pKa = 11.84 EE168 pKa = 3.88 WVHH171 pKa = 6.21 HH172 pKa = 5.8 SRR174 pKa = 11.84 SAEE177 pKa = 4.25 CYY179 pKa = 9.1 SAAAPAVGRR188 pKa = 11.84 LHH190 pKa = 7.69 DD191 pKa = 4.36 ARR193 pKa = 11.84 AEE195 pKa = 3.74 NDD197 pKa = 3.04 ARR199 pKa = 11.84 SFEE202 pKa = 4.38 VATPILTVADD212 pKa = 4.07 RR213 pKa = 11.84 SGARR217 pKa = 11.84 LFQRR221 pKa = 11.84 LPNRR225 pKa = 11.84 KK226 pKa = 8.75 RR227 pKa = 11.84 GTVEE231 pKa = 4.2 RR232 pKa = 11.84 AMQPLVPGDD241 pKa = 4.1 AVLCSDD247 pKa = 4.83 GGNGYY252 pKa = 10.43 KK253 pKa = 10.19 SLAAARR259 pKa = 11.84 GLVHH263 pKa = 6.49 FVVGSRR269 pKa = 11.84 PGTRR273 pKa = 11.84 VAAGCYY279 pKa = 9.58 HH280 pKa = 6.13 IQNVNSLHH288 pKa = 5.65 ARR290 pKa = 11.84 YY291 pKa = 9.74 GKK293 pKa = 10.22 FIRR296 pKa = 11.84 PFCGPATKK304 pKa = 10.48 NLNGYY309 pKa = 8.78 IRR311 pKa = 11.84 WLEE314 pKa = 3.69 VRR316 pKa = 11.84 LAGVRR321 pKa = 11.84 PAEE324 pKa = 4.29 VVRR327 pKa = 11.84 ASS329 pKa = 3.1
Molecular weight: 37.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.575
IPC_protein 10.467
Toseland 10.57
ProMoST 10.321
Dawson 10.687
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 10.789
Grimsley 10.745
Solomon 10.804
Lehninger 10.76
Nozaki 10.584
DTASelect 10.423
Thurlkill 10.584
EMBOSS 10.979
Sillero 10.628
Patrickios 10.452
IPC_peptide 10.804
IPC2_peptide 9.663
IPC2.peptide.svr19 8.572
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4315
0
4315
1295017
25
2773
300.1
32.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.386 ± 0.054
0.856 ± 0.012
6.189 ± 0.031
6.047 ± 0.033
3.725 ± 0.025
8.829 ± 0.033
2.008 ± 0.019
4.898 ± 0.027
2.712 ± 0.027
10.145 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.621 ± 0.018
2.277 ± 0.019
5.154 ± 0.026
2.694 ± 0.023
7.366 ± 0.035
5.256 ± 0.026
5.563 ± 0.025
7.699 ± 0.027
1.466 ± 0.015
2.109 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here