Cuscuta australis

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Convolvulaceae; Cuscuteae; Cuscuta; Cuscuta subgen. Grammica; Cuscuta sect. Cleistogrammica

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18107 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328DM74|A0A328DM74_9ASTE PSP domain-containing protein OS=Cuscuta australis OX=267555 GN=DM860_005044 PE=4 SV=1
MM1 pKa = 7.5HH2 pKa = 7.25FMYY5 pKa = 10.55SVAIAWAVAMWGRR18 pKa = 11.84AIYY21 pKa = 10.37LKK23 pKa = 9.16TICSAAASGCSPNDD37 pKa = 3.56PSPSPTIAGDD47 pKa = 3.53EE48 pKa = 4.19RR49 pKa = 11.84AGTVTDD55 pKa = 3.62ASEE58 pKa = 4.09NEE60 pKa = 4.26EE61 pKa = 3.93ASGDD65 pKa = 3.8SDD67 pKa = 4.1EE68 pKa = 5.9DD69 pKa = 4.38YY70 pKa = 11.35SDD72 pKa = 4.3SDD74 pKa = 3.26II75 pKa = 5.21

Molecular weight: 7.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328DF96|A0A328DF96_9ASTE Cytokinin riboside 5'-monophosphate phosphoribohydrolase OS=Cuscuta australis OX=267555 GN=DM860_011829 PE=3 SV=1
MM1 pKa = 7.81PSHH4 pKa = 6.22KK5 pKa = 9.72TFMIKK10 pKa = 10.18KK11 pKa = 9.5KK12 pKa = 9.82LAKK15 pKa = 9.7KK16 pKa = 9.71QRR18 pKa = 11.84QNRR21 pKa = 11.84PIPSWIRR28 pKa = 11.84MRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.47RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.81LGFF51 pKa = 3.81

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18107

0

18107

7463362

28

5453

412.2

45.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.007 ± 0.016

1.939 ± 0.008

5.317 ± 0.012

6.461 ± 0.02

4.193 ± 0.011

6.833 ± 0.018

2.511 ± 0.007

5.095 ± 0.012

5.975 ± 0.019

9.54 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.438 ± 0.008

4.285 ± 0.011

5.087 ± 0.018

3.467 ± 0.012

5.632 ± 0.012

8.893 ± 0.021

4.784 ± 0.01

6.533 ± 0.012

1.273 ± 0.007

2.737 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski