Rusa timorensis papillomavirus type 2
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2R2Z1A7|A0A2R2Z1A7_9PAPI Major capsid protein L1 OS=Rusa timorensis papillomavirus type 2 OX=1905556 GN=L1 PE=3 SV=1
MM1 pKa = 7.25 FVFLSVCVVTLIMATVNRR19 pKa = 11.84 RR20 pKa = 11.84 KK21 pKa = 9.87 RR22 pKa = 11.84 ASDD25 pKa = 2.84 WDD27 pKa = 3.87 LYY29 pKa = 9.78 RR30 pKa = 11.84 ACRR33 pKa = 11.84 GGADD37 pKa = 4.17 CISDD41 pKa = 3.59 VVNKK45 pKa = 10.35 YY46 pKa = 6.97 EE47 pKa = 4.71 HH48 pKa = 6.65 KK49 pKa = 9.43 TPADD53 pKa = 3.16 KK54 pKa = 10.92 VLQYY58 pKa = 11.11 GGAGVFLGGLGIGTGSGALGVRR80 pKa = 11.84 PGVTGPAEE88 pKa = 4.63 EE89 pKa = 4.69 IPLVTLGEE97 pKa = 4.29 RR98 pKa = 11.84 TTITDD103 pKa = 3.47 TGSGRR108 pKa = 11.84 TPSLGSTSFGGTSRR122 pKa = 11.84 FGSGFGGRR130 pKa = 11.84 IDD132 pKa = 5.37 LISAGRR138 pKa = 11.84 GRR140 pKa = 11.84 VTTSDD145 pKa = 3.3 VSVNTVDD152 pKa = 3.67 SVTPSVILPEE162 pKa = 4.31 TVPADD167 pKa = 3.63 PGIEE171 pKa = 3.9 LQVFTDD177 pKa = 3.78 VEE179 pKa = 4.56 DD180 pKa = 3.94 SVQVTVSSSVDD191 pKa = 3.23 EE192 pKa = 4.86 GEE194 pKa = 4.39 VAVLEE199 pKa = 4.68 VPATEE204 pKa = 4.3 SFGRR208 pKa = 11.84 PSQVTTRR215 pKa = 11.84 GGGGKK220 pKa = 8.06 VHH222 pKa = 7.25 HH223 pKa = 6.86 SRR225 pKa = 11.84 HH226 pKa = 4.92 VVPAEE231 pKa = 3.67 TGTILGEE238 pKa = 4.05 TSEE241 pKa = 4.32 TQNIFIEE248 pKa = 4.87 GSGVGDD254 pKa = 3.87 SYY256 pKa = 11.86 SEE258 pKa = 4.63 SIEE261 pKa = 3.73 LWTYY265 pKa = 10.08 SRR267 pKa = 11.84 PRR269 pKa = 11.84 YY270 pKa = 7.31 STPDD274 pKa = 2.98 SEE276 pKa = 4.71 PPVNRR281 pKa = 11.84 RR282 pKa = 11.84 GINNPFSKK290 pKa = 9.95 RR291 pKa = 11.84 YY292 pKa = 8.0 YY293 pKa = 9.57 KK294 pKa = 10.3 QVTVEE299 pKa = 4.61 DD300 pKa = 4.29 PQFLTEE306 pKa = 3.97 PQKK309 pKa = 10.92 LVSNVFEE316 pKa = 4.5 NPAFVDD322 pKa = 4.48 DD323 pKa = 4.74 VDD325 pKa = 5.37 DD326 pKa = 5.62 SIPFLDD332 pKa = 4.11 TDD334 pKa = 3.55 TRR336 pKa = 11.84 PYY338 pKa = 9.27 NTEE341 pKa = 3.75 SDD343 pKa = 3.73 FLDD346 pKa = 3.22 IGRR349 pKa = 11.84 LGRR352 pKa = 11.84 VQYY355 pKa = 9.37 TVSPGEE361 pKa = 3.84 GLGVSRR367 pKa = 11.84 IGTRR371 pKa = 11.84 FTIKK375 pKa = 9.26 TRR377 pKa = 11.84 SGVTIGEE384 pKa = 4.35 QVHH387 pKa = 5.04 YY388 pKa = 9.6 RR389 pKa = 11.84 YY390 pKa = 9.31 PLSPITGASEE400 pKa = 5.24 DD401 pKa = 3.88 IEE403 pKa = 4.3 LQNLVTGMSEE413 pKa = 4.38 GVLDD417 pKa = 4.04 SLNDD421 pKa = 3.9 TVVEE425 pKa = 4.14 TLNNAMLEE433 pKa = 4.32 SVDD436 pKa = 5.33 LDD438 pKa = 4.77 DD439 pKa = 5.92 ISDD442 pKa = 3.96 FLSVSSSVLEE452 pKa = 4.02 NEE454 pKa = 4.92 LMDD457 pKa = 3.93 NVEE460 pKa = 5.53 DD461 pKa = 3.57 ITFGQLAFRR470 pKa = 11.84 DD471 pKa = 3.76 EE472 pKa = 4.74 GEE474 pKa = 4.57 TEE476 pKa = 4.15 VFTYY480 pKa = 10.37 PDD482 pKa = 3.28 YY483 pKa = 11.9 SNTQKK488 pKa = 11.22 AGVTVISEE496 pKa = 4.49 DD497 pKa = 3.4 NVTGTKK503 pKa = 10.28 SGDD506 pKa = 3.53 VVNEE510 pKa = 3.93 TTINTHH516 pKa = 6.24 GNDD519 pKa = 3.2 SLIDD523 pKa = 3.53 VLVIDD528 pKa = 5.61 DD529 pKa = 3.99 YY530 pKa = 12.06 NFYY533 pKa = 10.85 LSYY536 pKa = 10.62 YY537 pKa = 8.61 LHH539 pKa = 7.28 PANFPKK545 pKa = 10.52 KK546 pKa = 9.79 KK547 pKa = 9.77 RR548 pKa = 11.84 RR549 pKa = 11.84 KK550 pKa = 8.06 LWYY553 pKa = 8.57 MM554 pKa = 3.99
Molecular weight: 60.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.527
IPC2_protein 4.469
IPC_protein 4.444
Toseland 4.279
ProMoST 4.558
Dawson 4.406
Bjellqvist 4.546
Wikipedia 4.291
Rodwell 4.279
Grimsley 4.177
Solomon 4.406
Lehninger 4.355
Nozaki 4.507
DTASelect 4.698
Thurlkill 4.279
EMBOSS 4.304
Sillero 4.558
Patrickios 3.846
IPC_peptide 4.406
IPC2_peptide 4.546
IPC2.peptide.svr19 4.5
Protein with the highest isoelectric point:
>tr|A0A2R2Z1A9|A0A2R2Z1A9_9PAPI Regulatory protein E2 OS=Rusa timorensis papillomavirus type 2 OX=1905556 GN=E2 PE=3 SV=1
MM1 pKa = 7.0 VAGPATTKK9 pKa = 10.5 CLPKK13 pKa = 10.7 DD14 pKa = 3.45 KK15 pKa = 10.94 GLGAITLTLKK25 pKa = 10.29 PLPGGDD31 pKa = 3.97 GVDD34 pKa = 3.62 IAQKK38 pKa = 10.48 APPAAQPGLKK48 pKa = 10.08 NFCQLHH54 pKa = 5.8 HH55 pKa = 6.86 KK56 pKa = 8.49 WRR58 pKa = 11.84 PASNLAIQRR67 pKa = 11.84 IPFADD72 pKa = 3.55 LTVKK76 pKa = 10.46 NYY78 pKa = 9.0 YY79 pKa = 9.76 VRR81 pKa = 11.84 SCCPLCGQSLNYY93 pKa = 9.67 CVHH96 pKa = 5.95 TNKK99 pKa = 10.01 TGIFSFEE106 pKa = 4.03 QLLSSCFSLVCPGCAEE122 pKa = 4.12 SS123 pKa = 4.31
Molecular weight: 13.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.096
IPC2_protein 7.907
IPC_protein 7.761
Toseland 7.234
ProMoST 8.317
Dawson 8.448
Bjellqvist 8.916
Wikipedia 8.346
Rodwell 8.463
Grimsley 7.19
Solomon 8.551
Lehninger 8.565
Nozaki 9.282
DTASelect 8.565
Thurlkill 8.624
EMBOSS 8.653
Sillero 9.004
Patrickios 3.427
IPC_peptide 8.536
IPC2_peptide 8.2
IPC2.peptide.svr19 8.241
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2183
123
586
436.6
48.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.413 ± 0.954
2.749 ± 0.733
6.093 ± 0.481
5.772 ± 0.52
4.169 ± 0.603
7.467 ± 0.956
1.787 ± 0.183
4.26 ± 0.616
5.36 ± 0.783
8.383 ± 0.423
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.878 ± 0.415
4.627 ± 0.263
5.589 ± 0.649
4.031 ± 0.499
5.314 ± 0.303
7.696 ± 0.717
7.1 ± 0.861
6.505 ± 1.337
1.42 ± 0.29
3.39 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here