Amino acid dipepetide frequency for Mycobacterium phage Thibault

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.895AlaAla: 10.895 ± 0.897
1.156AlaCys: 1.156 ± 0.226
6.452AlaAsp: 6.452 ± 0.444
7.791AlaGlu: 7.791 ± 0.604
3.287AlaPhe: 3.287 ± 0.31
7.121AlaGly: 7.121 ± 0.644
2.191AlaHis: 2.191 ± 0.298
5.143AlaIle: 5.143 ± 0.358
3.987AlaLys: 3.987 ± 0.347
8.673AlaLeu: 8.673 ± 0.599
3.074AlaMet: 3.074 ± 0.398
2.8AlaAsn: 2.8 ± 0.391
3.774AlaPro: 3.774 ± 0.328
2.678AlaGln: 2.678 ± 0.288
6.908AlaArg: 6.908 ± 0.462
5.387AlaSer: 5.387 ± 0.493
5.174AlaThr: 5.174 ± 0.468
6.147AlaVal: 6.147 ± 0.457
2.069AlaTrp: 2.069 ± 0.24
2.556AlaTyr: 2.556 ± 0.258
0.0AlaXaa: 0.0 ± 0.0
Cys
1.126CysAla: 1.126 ± 0.213
0.091CysCys: 0.091 ± 0.052
1.156CysAsp: 1.156 ± 0.207
0.974CysGlu: 0.974 ± 0.185
0.396CysPhe: 0.396 ± 0.1
2.039CysGly: 2.039 ± 0.298
0.548CysHis: 0.548 ± 0.132
0.578CysIle: 0.578 ± 0.146
0.7CysLys: 0.7 ± 0.201
1.217CysLeu: 1.217 ± 0.211
0.396CysMet: 0.396 ± 0.11
0.609CysAsn: 0.609 ± 0.143
0.67CysPro: 0.67 ± 0.179
0.426CysGln: 0.426 ± 0.119
1.126CysArg: 1.126 ± 0.243
0.73CysSer: 0.73 ± 0.154
0.67CysThr: 0.67 ± 0.132
1.004CysVal: 1.004 ± 0.195
0.213CysTrp: 0.213 ± 0.086
0.548CysTyr: 0.548 ± 0.13
0.0CysXaa: 0.0 ± 0.0
Asp
6.147AspAla: 6.147 ± 0.493
0.913AspCys: 0.913 ± 0.21
4.443AspAsp: 4.443 ± 0.38
4.991AspGlu: 4.991 ± 0.546
2.313AspPhe: 2.313 ± 0.235
6.421AspGly: 6.421 ± 0.494
2.161AspHis: 2.161 ± 0.278
2.922AspIle: 2.922 ± 0.301
2.739AspLys: 2.739 ± 0.315
5.691AspLeu: 5.691 ± 0.345
1.156AspMet: 1.156 ± 0.156
2.252AspAsn: 2.252 ± 0.272
3.439AspPro: 3.439 ± 0.345
1.917AspGln: 1.917 ± 0.263
3.987AspArg: 3.987 ± 0.384
2.952AspSer: 2.952 ± 0.334
3.135AspThr: 3.135 ± 0.34
4.23AspVal: 4.23 ± 0.444
1.796AspTrp: 1.796 ± 0.221
2.374AspTyr: 2.374 ± 0.238
0.0AspXaa: 0.0 ± 0.0
Glu
6.817GluAla: 6.817 ± 0.501
1.369GluCys: 1.369 ± 0.254
4.626GluAsp: 4.626 ± 0.431
5.326GluGlu: 5.326 ± 0.533
2.648GluPhe: 2.648 ± 0.298
3.895GluGly: 3.895 ± 0.359
1.369GluHis: 1.369 ± 0.199
4.23GluIle: 4.23 ± 0.387
2.648GluLys: 2.648 ± 0.338
6.543GluLeu: 6.543 ± 0.532
2.222GluMet: 2.222 ± 0.305
1.735GluAsn: 1.735 ± 0.244
3.804GluPro: 3.804 ± 0.369
2.313GluGln: 2.313 ± 0.243
5.234GluArg: 5.234 ± 0.471
3.439GluSer: 3.439 ± 0.341
2.952GluThr: 2.952 ± 0.332
5.021GluVal: 5.021 ± 0.392
1.278GluTrp: 1.278 ± 0.251
2.739GluTyr: 2.739 ± 0.309
0.0GluXaa: 0.0 ± 0.0
Phe
2.739PheAla: 2.739 ± 0.29
0.609PheCys: 0.609 ± 0.126
2.465PheAsp: 2.465 ± 0.26
2.039PheGlu: 2.039 ± 0.234
1.035PhePhe: 1.035 ± 0.177
3.135PheGly: 3.135 ± 0.372
0.578PheHis: 0.578 ± 0.15
1.491PheIle: 1.491 ± 0.198
1.096PheLys: 1.096 ± 0.197
2.374PheLeu: 2.374 ± 0.285
0.791PheMet: 0.791 ± 0.138
1.4PheAsn: 1.4 ± 0.253
1.765PhePro: 1.765 ± 0.239
1.004PheGln: 1.004 ± 0.21
1.917PheArg: 1.917 ± 0.27
2.191PheSer: 2.191 ± 0.276
2.191PheThr: 2.191 ± 0.249
2.13PheVal: 2.13 ± 0.248
0.517PheTrp: 0.517 ± 0.129
0.761PheTyr: 0.761 ± 0.154
0.0PheXaa: 0.0 ± 0.0
Gly
6.604GlyAla: 6.604 ± 0.615
1.552GlyCys: 1.552 ± 0.253
6.056GlyAsp: 6.056 ± 0.495
5.691GlyGlu: 5.691 ± 0.481
3.195GlyPhe: 3.195 ± 0.332
7.7GlyGly: 7.7 ± 1.209
2.1GlyHis: 2.1 ± 0.273
4.291GlyIle: 4.291 ± 0.344
4.139GlyLys: 4.139 ± 0.367
7.365GlyLeu: 7.365 ± 0.582
1.735GlyMet: 1.735 ± 0.246
2.952GlyAsn: 2.952 ± 0.354
3.804GlyPro: 3.804 ± 0.36
2.435GlyGln: 2.435 ± 0.369
6.026GlyArg: 6.026 ± 0.388
4.687GlySer: 4.687 ± 0.33
4.961GlyThr: 4.961 ± 0.409
4.869GlyVal: 4.869 ± 0.384
1.978GlyTrp: 1.978 ± 0.253
2.952GlyTyr: 2.952 ± 0.303
0.0GlyXaa: 0.0 ± 0.0
His
1.643HisAla: 1.643 ± 0.245
0.396HisCys: 0.396 ± 0.106
1.491HisAsp: 1.491 ± 0.226
1.735HisGlu: 1.735 ± 0.256
0.609HisPhe: 0.609 ± 0.146
2.282HisGly: 2.282 ± 0.274
0.913HisHis: 0.913 ± 0.195
1.126HisIle: 1.126 ± 0.208
0.67HisLys: 0.67 ± 0.142
2.404HisLeu: 2.404 ± 0.329
0.548HisMet: 0.548 ± 0.131
0.639HisAsn: 0.639 ± 0.159
1.43HisPro: 1.43 ± 0.218
0.883HisGln: 0.883 ± 0.156
2.039HisArg: 2.039 ± 0.298
0.913HisSer: 0.913 ± 0.168
0.7HisThr: 0.7 ± 0.159
1.278HisVal: 1.278 ± 0.22
0.639HisTrp: 0.639 ± 0.139
0.761HisTyr: 0.761 ± 0.16
0.0HisXaa: 0.0 ± 0.0
Ile
5.569IleAla: 5.569 ± 0.421
0.487IleCys: 0.487 ± 0.116
3.5IleAsp: 3.5 ± 0.382
3.682IleGlu: 3.682 ± 0.329
1.248IlePhe: 1.248 ± 0.195
4.078IleGly: 4.078 ± 0.464
1.217IleHis: 1.217 ± 0.224
1.917IleIle: 1.917 ± 0.275
1.917IleLys: 1.917 ± 0.221
3.682IleLeu: 3.682 ± 0.296
0.761IleMet: 0.761 ± 0.159
1.491IleAsn: 1.491 ± 0.244
3.135IlePro: 3.135 ± 0.234
1.704IleGln: 1.704 ± 0.207
3.348IleArg: 3.348 ± 0.285
2.252IleSer: 2.252 ± 0.244
2.769IleThr: 2.769 ± 0.297
3.5IleVal: 3.5 ± 0.317
0.67IleTrp: 0.67 ± 0.142
1.552IleTyr: 1.552 ± 0.203
0.0IleXaa: 0.0 ± 0.0
Lys
4.2LysAla: 4.2 ± 0.354
0.578LysCys: 0.578 ± 0.144
2.069LysAsp: 2.069 ± 0.301
2.191LysGlu: 2.191 ± 0.297
1.217LysPhe: 1.217 ± 0.159
3.165LysGly: 3.165 ± 0.32
1.156LysHis: 1.156 ± 0.195
1.796LysIle: 1.796 ± 0.257
2.039LysLys: 2.039 ± 0.294
3.652LysLeu: 3.652 ± 0.331
1.369LysMet: 1.369 ± 0.177
0.791LysAsn: 0.791 ± 0.144
2.709LysPro: 2.709 ± 0.261
1.4LysGln: 1.4 ± 0.195
3.165LysArg: 3.165 ± 0.351
2.1LysSer: 2.1 ± 0.343
1.674LysThr: 1.674 ± 0.212
3.378LysVal: 3.378 ± 0.38
1.096LysTrp: 1.096 ± 0.184
1.126LysTyr: 1.126 ± 0.176
0.0LysXaa: 0.0 ± 0.0
Leu
9.16LeuAla: 9.16 ± 0.531
0.822LeuCys: 0.822 ± 0.179
5.204LeuAsp: 5.204 ± 0.368
5.326LeuGlu: 5.326 ± 0.404
2.587LeuPhe: 2.587 ± 0.308
6.726LeuGly: 6.726 ± 0.565
1.887LeuHis: 1.887 ± 0.213
3.043LeuIle: 3.043 ± 0.361
3.195LeuLys: 3.195 ± 0.289
6.391LeuLeu: 6.391 ± 0.546
1.917LeuMet: 1.917 ± 0.245
3.135LeuAsn: 3.135 ± 0.271
4.687LeuPro: 4.687 ± 0.388
2.891LeuGln: 2.891 ± 0.363
5.417LeuArg: 5.417 ± 0.423
5.082LeuSer: 5.082 ± 0.39
5.448LeuThr: 5.448 ± 0.372
5.174LeuVal: 5.174 ± 0.331
1.309LeuTrp: 1.309 ± 0.18
1.978LeuTyr: 1.978 ± 0.293
0.0LeuXaa: 0.0 ± 0.0
Met
2.465MetAla: 2.465 ± 0.214
0.243MetCys: 0.243 ± 0.084
1.735MetAsp: 1.735 ± 0.248
1.187MetGlu: 1.187 ± 0.19
0.791MetPhe: 0.791 ± 0.158
1.704MetGly: 1.704 ± 0.245
0.396MetHis: 0.396 ± 0.094
1.278MetIle: 1.278 ± 0.168
1.156MetLys: 1.156 ± 0.179
1.248MetLeu: 1.248 ± 0.209
0.517MetMet: 0.517 ± 0.114
1.065MetAsn: 1.065 ± 0.16
1.217MetPro: 1.217 ± 0.188
0.609MetGln: 0.609 ± 0.14
1.187MetArg: 1.187 ± 0.194
2.556MetSer: 2.556 ± 0.307
2.313MetThr: 2.313 ± 0.276
1.309MetVal: 1.309 ± 0.19
0.517MetTrp: 0.517 ± 0.125
0.335MetTyr: 0.335 ± 0.096
0.0MetXaa: 0.0 ± 0.0
Asn
3.043AsnAla: 3.043 ± 0.342
0.335AsnCys: 0.335 ± 0.096
2.161AsnAsp: 2.161 ± 0.256
1.856AsnGlu: 1.856 ± 0.215
1.004AsnPhe: 1.004 ± 0.18
3.774AsnGly: 3.774 ± 0.295
0.822AsnHis: 0.822 ± 0.135
1.309AsnIle: 1.309 ± 0.228
1.339AsnLys: 1.339 ± 0.223
2.617AsnLeu: 2.617 ± 0.308
0.517AsnMet: 0.517 ± 0.13
0.761AsnAsn: 0.761 ± 0.153
2.496AsnPro: 2.496 ± 0.243
0.852AsnGln: 0.852 ± 0.202
2.435AsnArg: 2.435 ± 0.275
1.552AsnSer: 1.552 ± 0.223
1.704AsnThr: 1.704 ± 0.213
2.465AsnVal: 2.465 ± 0.258
0.974AsnTrp: 0.974 ± 0.187
0.791AsnTyr: 0.791 ± 0.162
0.0AsnXaa: 0.0 ± 0.0
Pro
4.748ProAla: 4.748 ± 0.471
0.7ProCys: 0.7 ± 0.14
3.53ProAsp: 3.53 ± 0.325
4.595ProGlu: 4.595 ± 0.433
1.978ProPhe: 1.978 ± 0.24
5.874ProGly: 5.874 ± 0.728
0.943ProHis: 0.943 ± 0.124
2.282ProIle: 2.282 ± 0.262
2.069ProLys: 2.069 ± 0.268
4.048ProLeu: 4.048 ± 0.323
1.248ProMet: 1.248 ± 0.219
2.252ProAsn: 2.252 ± 0.256
2.739ProPro: 2.739 ± 0.369
1.065ProGln: 1.065 ± 0.209
2.922ProArg: 2.922 ± 0.331
2.556ProSer: 2.556 ± 0.275
3.074ProThr: 3.074 ± 0.307
3.743ProVal: 3.743 ± 0.334
1.278ProTrp: 1.278 ± 0.178
1.369ProTyr: 1.369 ± 0.22
0.0ProXaa: 0.0 ± 0.0
Gln
3.317GlnAla: 3.317 ± 0.382
0.67GlnCys: 0.67 ± 0.143
1.339GlnAsp: 1.339 ± 0.194
1.856GlnGlu: 1.856 ± 0.244
1.187GlnPhe: 1.187 ± 0.199
2.191GlnGly: 2.191 ± 0.336
0.548GlnHis: 0.548 ± 0.117
1.856GlnIle: 1.856 ± 0.232
1.704GlnLys: 1.704 ± 0.327
2.556GlnLeu: 2.556 ± 0.309
0.974GlnMet: 0.974 ± 0.162
0.73GlnAsn: 0.73 ± 0.145
1.978GlnPro: 1.978 ± 0.289
1.217GlnGln: 1.217 ± 0.213
2.069GlnArg: 2.069 ± 0.256
1.643GlnSer: 1.643 ± 0.215
1.522GlnThr: 1.522 ± 0.241
2.161GlnVal: 2.161 ± 0.277
0.67GlnTrp: 0.67 ± 0.132
0.67GlnTyr: 0.67 ± 0.123
0.0GlnXaa: 0.0 ± 0.0
Arg
6.787ArgAla: 6.787 ± 0.593
1.461ArgCys: 1.461 ± 0.306
4.139ArgAsp: 4.139 ± 0.332
4.656ArgGlu: 4.656 ± 0.467
1.948ArgPhe: 1.948 ± 0.233
5.143ArgGly: 5.143 ± 0.371
1.156ArgHis: 1.156 ± 0.196
3.622ArgIle: 3.622 ± 0.369
3.195ArgLys: 3.195 ± 0.337
4.778ArgLeu: 4.778 ± 0.414
2.1ArgMet: 2.1 ± 0.281
2.252ArgAsn: 2.252 ± 0.275
3.104ArgPro: 3.104 ± 0.366
2.8ArgGln: 2.8 ± 0.323
5.63ArgArg: 5.63 ± 0.416
3.256ArgSer: 3.256 ± 0.274
3.5ArgThr: 3.5 ± 0.405
4.626ArgVal: 4.626 ± 0.381
2.191ArgTrp: 2.191 ± 0.255
2.465ArgTyr: 2.465 ± 0.273
0.0ArgXaa: 0.0 ± 0.0
Ser
5.904SerAla: 5.904 ± 0.468
0.791SerCys: 0.791 ± 0.141
3.348SerAsp: 3.348 ± 0.324
4.017SerGlu: 4.017 ± 0.42
1.613SerPhe: 1.613 ± 0.217
5.387SerGly: 5.387 ± 0.536
1.217SerHis: 1.217 ± 0.219
2.617SerIle: 2.617 ± 0.295
2.1SerLys: 2.1 ± 0.256
4.474SerLeu: 4.474 ± 0.394
1.461SerMet: 1.461 ± 0.159
1.856SerAsn: 1.856 ± 0.22
2.769SerPro: 2.769 ± 0.258
1.248SerGln: 1.248 ± 0.218
3.5SerArg: 3.5 ± 0.392
3.195SerSer: 3.195 ± 0.338
2.922SerThr: 2.922 ± 0.303
3.987SerVal: 3.987 ± 0.389
1.369SerTrp: 1.369 ± 0.236
1.522SerTyr: 1.522 ± 0.199
0.0SerXaa: 0.0 ± 0.0
Thr
4.717ThrAla: 4.717 ± 0.441
1.004ThrCys: 1.004 ± 0.196
3.53ThrAsp: 3.53 ± 0.347
3.287ThrGlu: 3.287 ± 0.317
2.069ThrPhe: 2.069 ± 0.242
5.021ThrGly: 5.021 ± 0.42
1.187ThrHis: 1.187 ± 0.204
3.135ThrIle: 3.135 ± 0.373
1.796ThrLys: 1.796 ± 0.267
4.626ThrLeu: 4.626 ± 0.295
0.883ThrMet: 0.883 ± 0.177
1.613ThrAsn: 1.613 ± 0.263
4.23ThrPro: 4.23 ± 0.411
1.339ThrGln: 1.339 ± 0.178
3.195ThrArg: 3.195 ± 0.329
3.287ThrSer: 3.287 ± 0.388
2.709ThrThr: 2.709 ± 0.42
4.321ThrVal: 4.321 ± 0.424
1.583ThrTrp: 1.583 ± 0.252
1.674ThrTyr: 1.674 ± 0.231
0.0ThrXaa: 0.0 ± 0.0
Val
6.665ValAla: 6.665 ± 0.485
0.913ValCys: 0.913 ± 0.188
5.478ValAsp: 5.478 ± 0.411
5.539ValGlu: 5.539 ± 0.432
1.735ValPhe: 1.735 ± 0.205
5.143ValGly: 5.143 ± 0.46
1.309ValHis: 1.309 ± 0.242
3.804ValIle: 3.804 ± 0.36
2.587ValLys: 2.587 ± 0.261
4.535ValLeu: 4.535 ± 0.417
1.248ValMet: 1.248 ± 0.178
2.922ValAsn: 2.922 ± 0.264
3.226ValPro: 3.226 ± 0.285
2.1ValGln: 2.1 ± 0.298
4.382ValArg: 4.382 ± 0.38
4.382ValSer: 4.382 ± 0.365
4.382ValThr: 4.382 ± 0.373
5.143ValVal: 5.143 ± 0.494
1.126ValTrp: 1.126 ± 0.192
1.765ValTyr: 1.765 ± 0.239
0.0ValXaa: 0.0 ± 0.0
Trp
1.856TrpAla: 1.856 ± 0.214
0.426TrpCys: 0.426 ± 0.119
1.248TrpAsp: 1.248 ± 0.185
1.217TrpGlu: 1.217 ± 0.169
0.639TrpPhe: 0.639 ± 0.156
1.552TrpGly: 1.552 ± 0.217
0.761TrpHis: 0.761 ± 0.147
1.035TrpIle: 1.035 ± 0.167
0.7TrpLys: 0.7 ± 0.147
1.978TrpLeu: 1.978 ± 0.254
0.548TrpMet: 0.548 ± 0.14
0.67TrpAsn: 0.67 ± 0.137
0.913TrpPro: 0.913 ± 0.163
0.791TrpGln: 0.791 ± 0.165
1.887TrpArg: 1.887 ± 0.272
1.796TrpSer: 1.796 ± 0.218
1.613TrpThr: 1.613 ± 0.255
1.735TrpVal: 1.735 ± 0.217
0.852TrpTrp: 0.852 ± 0.179
0.548TrpTyr: 0.548 ± 0.133
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.861TyrAla: 2.861 ± 0.276
0.7TyrCys: 0.7 ± 0.147
2.009TyrAsp: 2.009 ± 0.238
2.252TyrGlu: 2.252 ± 0.228
0.73TyrPhe: 0.73 ± 0.14
2.739TyrGly: 2.739 ± 0.304
0.639TyrHis: 0.639 ± 0.144
1.004TyrIle: 1.004 ± 0.19
1.096TyrLys: 1.096 ± 0.188
2.526TyrLeu: 2.526 ± 0.28
0.335TyrMet: 0.335 ± 0.116
0.883TyrAsn: 0.883 ± 0.161
1.126TyrPro: 1.126 ± 0.199
1.187TyrGln: 1.187 ± 0.182
2.465TyrArg: 2.465 ± 0.304
1.339TyrSer: 1.339 ± 0.196
1.796TyrThr: 1.796 ± 0.225
2.161TyrVal: 2.161 ± 0.282
0.639TyrTrp: 0.639 ± 0.126
0.73TyrTyr: 0.73 ± 0.156
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 216 proteins (32860 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski