Torque teno mini virus 5
Average proteome isoelectric point is 7.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9DUB5|Q9DUB5_9VIRU Uncharacterized protein OS=Torque teno mini virus 5 OX=687373 PE=4 SV=1
MM1 pKa = 7.32 SSCYY5 pKa = 10.23 KK6 pKa = 8.0 PTYY9 pKa = 10.37 YY10 pKa = 9.03 NTKK13 pKa = 9.93 GKK15 pKa = 7.37 QTQWINDD22 pKa = 3.22 IHH24 pKa = 6.18 CTHH27 pKa = 7.23 DD28 pKa = 3.63 LWCSCDD34 pKa = 3.52 HH35 pKa = 5.86 VLKK38 pKa = 10.89 HH39 pKa = 6.22 LLLSIAEE46 pKa = 4.05 RR47 pKa = 11.84 NEE49 pKa = 3.91 KK50 pKa = 10.15 IEE52 pKa = 3.94 LTKK55 pKa = 10.79 EE56 pKa = 3.74 EE57 pKa = 4.5 KK58 pKa = 10.57 KK59 pKa = 10.38 QVLQCLTTTEE69 pKa = 4.82 EE70 pKa = 3.94 ITTQNGDD77 pKa = 4.01 DD78 pKa = 4.21 GDD80 pKa = 4.27 LDD82 pKa = 4.86 EE83 pKa = 6.54 IGLDD87 pKa = 3.53 AIFAEE92 pKa = 4.91 EE93 pKa = 4.1 LTEE96 pKa = 4.73 DD97 pKa = 3.68 AGG99 pKa = 4.0
Molecular weight: 11.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.404
IPC2_protein 4.546
IPC_protein 4.444
Toseland 4.291
ProMoST 4.558
Dawson 4.393
Bjellqvist 4.546
Wikipedia 4.279
Rodwell 4.291
Grimsley 4.202
Solomon 4.393
Lehninger 4.355
Nozaki 4.507
DTASelect 4.66
Thurlkill 4.304
EMBOSS 4.291
Sillero 4.571
Patrickios 3.223
IPC_peptide 4.406
IPC2_peptide 4.558
IPC2.peptide.svr19 4.496
Protein with the highest isoelectric point:
>tr|Q9DUB4|Q9DUB4_9VIRU Capsid protein OS=Torque teno mini virus 5 OX=687373 PE=3 SV=1
MM1 pKa = 7.47 LKK3 pKa = 9.9 ILPTRR8 pKa = 11.84 ISFLYY13 pKa = 9.54 PVTSMKK19 pKa = 9.83 QLRR22 pKa = 11.84 YY23 pKa = 9.1 RR24 pKa = 11.84 VQGQPPKK31 pKa = 10.73 ASYY34 pKa = 8.46 THH36 pKa = 6.45 LTSDD40 pKa = 4.89 MEE42 pKa = 4.43 TTQQKK47 pKa = 10.34 LLQEE51 pKa = 4.3 LQRR54 pKa = 11.84 TGQLKK59 pKa = 10.63 KK60 pKa = 10.97 LFLKK64 pKa = 10.32 LQNQSQQNYY73 pKa = 8.34 SNKK76 pKa = 9.19 CSSFSKK82 pKa = 9.49 HH83 pKa = 3.97 QKK85 pKa = 8.48 KK86 pKa = 9.58 RR87 pKa = 11.84 RR88 pKa = 11.84 RR89 pKa = 11.84 KK90 pKa = 8.33 KK91 pKa = 10.29 KK92 pKa = 9.81 KK93 pKa = 9.54 RR94 pKa = 11.84 CTSTSNSSDD103 pKa = 3.14 NSSSSTEE110 pKa = 4.04 SEE112 pKa = 4.06
Molecular weight: 13.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.257
IPC2_protein 9.809
IPC_protein 9.999
Toseland 10.73
ProMoST 10.248
Dawson 10.818
Bjellqvist 10.438
Wikipedia 10.95
Rodwell 11.403
Grimsley 10.847
Solomon 10.862
Lehninger 10.847
Nozaki 10.701
DTASelect 10.423
Thurlkill 10.716
EMBOSS 11.111
Sillero 10.73
Patrickios 11.125
IPC_peptide 10.877
IPC2_peptide 9.121
IPC2.peptide.svr19 8.634
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
882
99
671
294.0
34.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.494 ± 0.433
1.701 ± 0.788
4.082 ± 0.968
5.782 ± 1.025
4.762 ± 1.352
3.628 ± 0.491
2.494 ± 0.299
5.669 ± 1.097
8.05 ± 1.713
7.256 ± 1.76
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.927 ± 0.215
6.122 ± 0.89
6.122 ± 1.766
8.277 ± 0.902
5.102 ± 0.798
7.029 ± 2.577
8.957 ± 0.492
2.041 ± 0.084
2.381 ± 0.811
6.122 ± 1.348
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here