Massilia namucuonensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Massilia

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7034 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I7FS64|A0A1I7FS64_9BURK DUF4214 domain-containing protein OS=Massilia namucuonensis OX=1035707 GN=SAMN05216552_1002247 PE=4 SV=1
MM1 pKa = 7.43TAFKK5 pKa = 9.91WVAGEE10 pKa = 4.27TKK12 pKa = 10.3SIQYY16 pKa = 10.41AAGDD20 pKa = 3.82TLEE23 pKa = 4.59LSGIAASQLEE33 pKa = 4.38LSVADD38 pKa = 4.23GRR40 pKa = 11.84LLLAAGALGTLTLTPVAGGALTAASLALTFPDD72 pKa = 3.32GGRR75 pKa = 11.84YY76 pKa = 8.78IPAGPDD82 pKa = 2.96ALTEE86 pKa = 4.5GGDD89 pKa = 3.56GGDD92 pKa = 3.79YY93 pKa = 11.18LLGDD97 pKa = 3.82DD98 pKa = 4.68QGNFVDD104 pKa = 5.77AGLGDD109 pKa = 3.89DD110 pKa = 3.78RR111 pKa = 11.84VEE113 pKa = 4.4GGGDD117 pKa = 3.46GDD119 pKa = 4.18YY120 pKa = 11.23LIGGAGDD127 pKa = 4.28DD128 pKa = 4.27LLLGGGGDD136 pKa = 4.25DD137 pKa = 5.02LLAGGDD143 pKa = 4.11GRR145 pKa = 11.84DD146 pKa = 3.72TLDD149 pKa = 4.12GGDD152 pKa = 3.96GANLLLGQKK161 pKa = 10.55GDD163 pKa = 3.68DD164 pKa = 3.6TYY166 pKa = 11.64LVHH169 pKa = 6.96SQDD172 pKa = 5.5DD173 pKa = 4.4YY174 pKa = 11.64ISDD177 pKa = 3.62SGGVDD182 pKa = 3.03GGVINADD189 pKa = 3.04WYY191 pKa = 9.0KK192 pKa = 10.04TADD195 pKa = 3.66EE196 pKa = 4.47VEE198 pKa = 4.02NWTWAPGVQRR208 pKa = 11.84LPYY211 pKa = 9.53WVDD214 pKa = 3.21ALTADD219 pKa = 3.83YY220 pKa = 10.85APYY223 pKa = 10.58VAMLVRR229 pKa = 11.84TAGRR233 pKa = 11.84SVHH236 pKa = 6.07YY237 pKa = 10.09SFPTTPADD245 pKa = 4.37FFTDD249 pKa = 3.2DD250 pKa = 4.36DD251 pKa = 4.55RR252 pKa = 11.84RR253 pKa = 11.84DD254 pKa = 3.75FLPFTEE260 pKa = 4.72DD261 pKa = 2.84QIAYY265 pKa = 6.38TRR267 pKa = 11.84KK268 pKa = 8.23ALAYY272 pKa = 9.53IEE274 pKa = 4.32TVLNIKK280 pKa = 10.35FEE282 pKa = 4.38EE283 pKa = 4.44TDD285 pKa = 3.94SPDD288 pKa = 3.29QPLSIVFGNNRR299 pKa = 11.84QEE301 pKa = 4.85DD302 pKa = 3.81SAAYY306 pKa = 10.16ANSLHH311 pKa = 7.49DD312 pKa = 4.99DD313 pKa = 4.05FGSPLMLSEE322 pKa = 5.42DD323 pKa = 4.38FLVMHH328 pKa = 7.19PSRR331 pKa = 11.84DD332 pKa = 3.94DD333 pKa = 3.88GDD335 pKa = 3.96SLYY338 pKa = 11.3SLLMHH343 pKa = 6.8EE344 pKa = 5.41LGHH347 pKa = 6.39SLGLKK352 pKa = 10.14HH353 pKa = 6.65PFAAPDD359 pKa = 3.46AYY361 pKa = 10.4GGEE364 pKa = 4.35IGAGPYY370 pKa = 10.01LGSGEE375 pKa = 4.66DD376 pKa = 3.46QTDD379 pKa = 3.6ATVMSYY385 pKa = 10.0TEE387 pKa = 4.64GEE389 pKa = 3.91LAQYY393 pKa = 11.3DD394 pKa = 4.12RR395 pKa = 11.84FSPLDD400 pKa = 3.44IAALQYY406 pKa = 10.98LYY408 pKa = 11.04GVAPGATGARR418 pKa = 11.84THH420 pKa = 6.15VLVATAANMIWVGAGLNIIDD440 pKa = 4.59GAHH443 pKa = 6.0LTADD447 pKa = 3.5LTLNLEE453 pKa = 4.52PGHH456 pKa = 6.59WSHH459 pKa = 7.55IGAKK463 pKa = 10.07ADD465 pKa = 3.87TITAAGQITINFGSVIVGAKK485 pKa = 10.01GGAGNDD491 pKa = 3.47ALTGNAISNEE501 pKa = 3.97LFGGAGNDD509 pKa = 3.83TLSGGASDD517 pKa = 5.68DD518 pKa = 3.85YY519 pKa = 11.74LFGGAGNDD527 pKa = 3.5ILSGGADD534 pKa = 3.64PDD536 pKa = 4.19YY537 pKa = 11.34LVGDD541 pKa = 4.98AGNDD545 pKa = 3.69MLSGGADD552 pKa = 3.45PDD554 pKa = 4.08YY555 pKa = 11.46LFGEE559 pKa = 4.74AGNDD563 pKa = 3.7TLSGGAEE570 pKa = 3.81ADD572 pKa = 3.57QLFGGAGVDD581 pKa = 3.36TAVFTGARR589 pKa = 11.84RR590 pKa = 11.84AYY592 pKa = 10.34DD593 pKa = 3.55LAWQNEE599 pKa = 4.07ALTVSALAGMDD610 pKa = 3.57GTDD613 pKa = 3.15TLNDD617 pKa = 3.34VEE619 pKa = 4.72RR620 pKa = 11.84LRR622 pKa = 11.84FADD625 pKa = 4.52GMLALDD631 pKa = 3.98VGGDD635 pKa = 3.69GAGGQVYY642 pKa = 10.17RR643 pKa = 11.84LYY645 pKa = 10.66QAAFDD650 pKa = 4.52RR651 pKa = 11.84KK652 pKa = 9.31PDD654 pKa = 3.76AAGVGYY660 pKa = 9.55WLAQADD666 pKa = 4.04QGAGMAGIAEE676 pKa = 4.19SFLRR680 pKa = 11.84SEE682 pKa = 4.24EE683 pKa = 3.85FARR686 pKa = 11.84LYY688 pKa = 11.09GGANPTDD695 pKa = 3.24AAFVAKK701 pKa = 10.39LYY703 pKa = 11.1SNVLHH708 pKa = 6.98RR709 pKa = 11.84AYY711 pKa = 10.58DD712 pKa = 3.55QTGYY716 pKa = 11.1DD717 pKa = 3.32YY718 pKa = 7.49WTWTLAAGASRR729 pKa = 11.84AEE731 pKa = 4.12VLLSFAAGEE740 pKa = 4.01EE741 pKa = 4.41NYY743 pKa = 10.2QQVAAIIANGYY754 pKa = 9.45EE755 pKa = 4.02YY756 pKa = 11.36AHH758 pKa = 6.52YY759 pKa = 10.67GG760 pKa = 3.38

Molecular weight: 79.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I7M7E5|A0A1I7M7E5_9BURK Uncharacterized protein OS=Massilia namucuonensis OX=1035707 GN=SAMN05216552_10725 PE=4 SV=1
II1 pKa = 7.27RR2 pKa = 11.84VKK4 pKa = 10.56LLKK7 pKa = 10.22IGAAIVRR14 pKa = 11.84NTRR17 pKa = 11.84RR18 pKa = 11.84VRR20 pKa = 11.84VLLASQHH27 pKa = 5.35PLKK30 pKa = 10.79HH31 pKa = 5.95VFISAARR38 pKa = 11.84AMAPP42 pKa = 3.32

Molecular weight: 4.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7034

0

7034

2567245

27

4738

365.0

39.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.327 ± 0.059

0.857 ± 0.009

5.392 ± 0.02

5.201 ± 0.029

3.416 ± 0.019

8.691 ± 0.038

2.167 ± 0.014

4.327 ± 0.023

3.47 ± 0.028

10.329 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.517 ± 0.015

3.021 ± 0.021

5.088 ± 0.026

3.733 ± 0.023

6.746 ± 0.037

5.398 ± 0.025

5.189 ± 0.037

7.173 ± 0.024

1.364 ± 0.012

2.593 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski