Amino acid dipepetide frequency for Virgibacillus necropolis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.087AlaAla: 5.087 ± 0.092	0.614AlaCys: 0.614 ± 0.023	3.366AlaAsp: 3.366 ± 0.063	4.254AlaGlu: 4.254 ± 0.062	3.258AlaPhe: 3.258 ± 0.059	5.076AlaGly: 5.076 ± 0.077	1.253AlaHis: 1.253 ± 0.034	6.319AlaIle: 6.319 ± 0.082	4.648AlaLys: 4.648 ± 0.064	6.857AlaLeu: 6.857 ± 0.092	1.951AlaMet: 1.951 ± 0.043	2.962AlaAsn: 2.962 ± 0.054	2.025AlaPro: 2.025 ± 0.043	2.007AlaGln: 2.007 ± 0.045	2.427AlaArg: 2.427 ± 0.044	4.089AlaSer: 4.089 ± 0.063	3.842AlaThr: 3.842 ± 0.064	5.149AlaVal: 5.149 ± 0.076	0.633AlaTrp: 0.633 ± 0.023	2.325AlaTyr: 2.325 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.402CysAla: 0.402 ± 0.017	0.079CysCys: 0.079 ± 0.01	0.42CysAsp: 0.42 ± 0.023	0.42CysGlu: 0.42 ± 0.02	0.307CysPhe: 0.307 ± 0.016	0.662CysGly: 0.662 ± 0.024	0.185CysHis: 0.185 ± 0.011	0.511CysIle: 0.511 ± 0.023	0.383CysLys: 0.383 ± 0.018	0.615CysLeu: 0.615 ± 0.021	0.196CysMet: 0.196 ± 0.013	0.322CysAsn: 0.322 ± 0.015	0.323CysPro: 0.323 ± 0.017	0.24CysGln: 0.24 ± 0.014	0.22CysArg: 0.22 ± 0.014	0.499CysSer: 0.499 ± 0.019	0.393CysThr: 0.393 ± 0.018	0.394CysVal: 0.394 ± 0.019	0.065CysTrp: 0.065 ± 0.008	0.235CysTyr: 0.235 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.42AspAla: 3.42 ± 0.06	0.392AspCys: 0.392 ± 0.021	2.794AspAsp: 2.794 ± 0.06	4.327AspGlu: 4.327 ± 0.074	2.41AspPhe: 2.41 ± 0.046	3.513AspGly: 3.513 ± 0.057	1.207AspHis: 1.207 ± 0.03	4.241AspIle: 4.241 ± 0.06	3.684AspLys: 3.684 ± 0.059	5.104AspLeu: 5.104 ± 0.061	1.382AspMet: 1.382 ± 0.038	2.249AspAsn: 2.249 ± 0.042	2.141AspPro: 2.141 ± 0.046	2.062AspGln: 2.062 ± 0.044	2.162AspArg: 2.162 ± 0.041	2.889AspSer: 2.889 ± 0.052	2.654AspThr: 2.654 ± 0.049	3.922AspVal: 3.922 ± 0.062	0.623AspTrp: 0.623 ± 0.022	2.282AspTyr: 2.282 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	4.942GluAla: 4.942 ± 0.079	0.358GluCys: 0.358 ± 0.02	3.786GluAsp: 3.786 ± 0.074	6.236GluGlu: 6.236 ± 0.093	2.563GluPhe: 2.563 ± 0.045	4.127GluGly: 4.127 ± 0.06	1.395GluHis: 1.395 ± 0.037	5.562GluIle: 5.562 ± 0.073	6.656GluLys: 6.656 ± 0.082	6.747GluLeu: 6.747 ± 0.08	2.172GluMet: 2.172 ± 0.046	3.899GluAsn: 3.899 ± 0.059	1.892GluPro: 1.892 ± 0.046	3.152GluGln: 3.152 ± 0.061	3.124GluArg: 3.124 ± 0.052	3.885GluSer: 3.885 ± 0.063	3.923GluThr: 3.923 ± 0.063	4.951GluVal: 4.951 ± 0.083	0.784GluTrp: 0.784 ± 0.026	2.123GluTyr: 2.123 ± 0.045	0.0GluXaa: 0.0 ± 0.0
Phe	3.058PheAla: 3.058 ± 0.054	0.322PheCys: 0.322 ± 0.016	2.458PheAsp: 2.458 ± 0.049	2.7PheGlu: 2.7 ± 0.052	2.303PhePhe: 2.303 ± 0.06	3.463PheGly: 3.463 ± 0.065	1.022PheHis: 1.022 ± 0.029	4.091PheIle: 4.091 ± 0.075	2.363PheLys: 2.363 ± 0.049	4.43PheLeu: 4.43 ± 0.082	1.187PheMet: 1.187 ± 0.033	1.955PheAsn: 1.955 ± 0.04	1.679PhePro: 1.679 ± 0.042	1.529PheGln: 1.529 ± 0.038	1.461PheArg: 1.461 ± 0.035	3.224PheSer: 3.224 ± 0.059	2.733PheThr: 2.733 ± 0.05	3.231PheVal: 3.231 ± 0.063	0.49PheTrp: 0.49 ± 0.025	1.716PheTyr: 1.716 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	4.844GlyAla: 4.844 ± 0.095	0.589GlyCys: 0.589 ± 0.025	3.213GlyAsp: 3.213 ± 0.058	4.337GlyGlu: 4.337 ± 0.068	3.529GlyPhe: 3.529 ± 0.068	4.998GlyGly: 4.998 ± 0.087	1.367GlyHis: 1.367 ± 0.038	6.398GlyIle: 6.398 ± 0.081	4.972GlyLys: 4.972 ± 0.067	6.611GlyLeu: 6.611 ± 0.099	2.183GlyMet: 2.183 ± 0.046	2.926GlyAsn: 2.926 ± 0.051	1.829GlyPro: 1.829 ± 0.04	2.009GlyGln: 2.009 ± 0.042	2.342GlyArg: 2.342 ± 0.045	4.374GlySer: 4.374 ± 0.059	4.085GlyThr: 4.085 ± 0.06	5.273GlyVal: 5.273 ± 0.074	0.848GlyTrp: 0.848 ± 0.028	2.798GlyTyr: 2.798 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	1.441HisAla: 1.441 ± 0.038	0.191HisCys: 0.191 ± 0.012	1.133HisAsp: 1.133 ± 0.034	1.343HisGlu: 1.343 ± 0.033	1.028HisPhe: 1.028 ± 0.027	1.476HisGly: 1.476 ± 0.04	0.637HisHis: 0.637 ± 0.025	1.506HisIle: 1.506 ± 0.036	1.131HisLys: 1.131 ± 0.032	1.992HisLeu: 1.992 ± 0.052	0.536HisMet: 0.536 ± 0.021	0.845HisAsn: 0.845 ± 0.028	1.075HisPro: 1.075 ± 0.028	0.792HisGln: 0.792 ± 0.025	0.783HisArg: 0.783 ± 0.026	1.128HisSer: 1.128 ± 0.031	1.136HisThr: 1.136 ± 0.031	1.545HisVal: 1.545 ± 0.04	0.205HisTrp: 0.205 ± 0.013	0.827HisTyr: 0.827 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	6.346IleAla: 6.346 ± 0.091	0.672IleCys: 0.672 ± 0.026	4.67IleAsp: 4.67 ± 0.065	5.668IleGlu: 5.668 ± 0.082	3.49IlePhe: 3.49 ± 0.069	6.451IleGly: 6.451 ± 0.103	1.739IleHis: 1.739 ± 0.044	6.646IleIle: 6.646 ± 0.11	4.953IleLys: 4.953 ± 0.077	7.149IleLeu: 7.149 ± 0.103	1.956IleMet: 1.956 ± 0.044	3.784IleAsn: 3.784 ± 0.057	3.594IlePro: 3.594 ± 0.048	2.839IleGln: 2.839 ± 0.053	2.949IleArg: 2.949 ± 0.05	5.145IleSer: 5.145 ± 0.073	4.716IleThr: 4.716 ± 0.07	5.97IleVal: 5.97 ± 0.078	0.692IleTrp: 0.692 ± 0.027	2.469IleTyr: 2.469 ± 0.056	0.0IleXaa: 0.0 ± 0.0
Lys	4.433LysAla: 4.433 ± 0.059	0.305LysCys: 0.305 ± 0.018	4.054LysAsp: 4.054 ± 0.06	6.764LysGlu: 6.764 ± 0.088	1.973LysPhe: 1.973 ± 0.045	4.248LysGly: 4.248 ± 0.06	1.355LysHis: 1.355 ± 0.033	4.713LysIle: 4.713 ± 0.069	6.0LysLys: 6.0 ± 0.083	5.934LysLeu: 5.934 ± 0.067	2.176LysMet: 2.176 ± 0.04	3.615LysAsn: 3.615 ± 0.067	2.276LysPro: 2.276 ± 0.047	3.199LysGln: 3.199 ± 0.063	3.14LysArg: 3.14 ± 0.059	3.961LysSer: 3.961 ± 0.071	3.596LysThr: 3.596 ± 0.064	4.64LysVal: 4.64 ± 0.07	0.815LysTrp: 0.815 ± 0.028	2.185LysTyr: 2.185 ± 0.048	0.0LysXaa: 0.0 ± 0.0
Leu	7.085LeuAla: 7.085 ± 0.096	0.595LeuCys: 0.595 ± 0.022	4.881LeuAsp: 4.881 ± 0.068	6.266LeuGlu: 6.266 ± 0.084	4.846LeuPhe: 4.846 ± 0.082	6.53LeuGly: 6.53 ± 0.095	1.899LeuHis: 1.899 ± 0.037	7.65LeuIle: 7.65 ± 0.113	6.118LeuLys: 6.118 ± 0.088	9.607LeuLeu: 9.607 ± 0.119	2.498LeuMet: 2.498 ± 0.048	4.379LeuAsn: 4.379 ± 0.063	3.81LeuPro: 3.81 ± 0.062	3.296LeuGln: 3.296 ± 0.051	3.239LeuArg: 3.239 ± 0.051	6.483LeuSer: 6.483 ± 0.081	5.652LeuThr: 5.652 ± 0.072	6.425LeuVal: 6.425 ± 0.084	0.8LeuTrp: 0.8 ± 0.027	3.092LeuTyr: 3.092 ± 0.058	0.0LeuXaa: 0.0 ± 0.0
Met	1.949MetAla: 1.949 ± 0.042	0.141MetCys: 0.141 ± 0.01	1.592MetAsp: 1.592 ± 0.039	2.087MetGlu: 2.087 ± 0.045	1.08MetPhe: 1.08 ± 0.032	1.834MetGly: 1.834 ± 0.048	0.471MetHis: 0.471 ± 0.019	2.205MetIle: 2.205 ± 0.048	2.41MetLys: 2.41 ± 0.049	2.578MetLeu: 2.578 ± 0.05	0.816MetMet: 0.816 ± 0.027	1.557MetAsn: 1.557 ± 0.042	0.955MetPro: 0.955 ± 0.028	0.946MetGln: 0.946 ± 0.03	1.034MetArg: 1.034 ± 0.033	1.668MetSer: 1.668 ± 0.04	1.619MetThr: 1.619 ± 0.038	1.842MetVal: 1.842 ± 0.042	0.199MetTrp: 0.199 ± 0.013	0.784MetTyr: 0.784 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	2.751AsnAla: 2.751 ± 0.042	0.332AsnCys: 0.332 ± 0.017	2.624AsnAsp: 2.624 ± 0.042	3.791AsnGlu: 3.791 ± 0.069	1.856AsnPhe: 1.856 ± 0.044	3.393AsnGly: 3.393 ± 0.059	1.123AsnHis: 1.123 ± 0.031	3.391AsnIle: 3.391 ± 0.057	3.397AsnLys: 3.397 ± 0.064	4.097AsnLeu: 4.097 ± 0.06	1.282AsnMet: 1.282 ± 0.034	2.363AsnAsn: 2.363 ± 0.047	2.207AsnPro: 2.207 ± 0.042	2.142AsnGln: 2.142 ± 0.052	2.041AsnArg: 2.041 ± 0.043	2.365AsnSer: 2.365 ± 0.049	2.374AsnThr: 2.374 ± 0.052	3.274AsnVal: 3.274 ± 0.055	0.552AsnTrp: 0.552 ± 0.023	1.784AsnTyr: 1.784 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	2.181ProAla: 2.181 ± 0.048	0.217ProCys: 0.217 ± 0.015	2.139ProAsp: 2.139 ± 0.045	2.992ProGlu: 2.992 ± 0.054	1.976ProPhe: 1.976 ± 0.044	2.365ProGly: 2.365 ± 0.053	0.803ProHis: 0.803 ± 0.028	3.006ProIle: 3.006 ± 0.055	2.227ProLys: 2.227 ± 0.046	3.493ProLeu: 3.493 ± 0.052	0.84ProMet: 0.84 ± 0.026	1.716ProAsn: 1.716 ± 0.04	0.98ProPro: 0.98 ± 0.031	0.951ProGln: 0.951 ± 0.03	1.025ProArg: 1.025 ± 0.03	2.329ProSer: 2.329 ± 0.052	2.122ProThr: 2.122 ± 0.039	2.828ProVal: 2.828 ± 0.052	0.367ProTrp: 0.367 ± 0.019	1.36ProTyr: 1.36 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	2.67GlnAla: 2.67 ± 0.051	0.164GlnCys: 0.164 ± 0.012	1.781GlnAsp: 1.781 ± 0.041	2.744GlnGlu: 2.744 ± 0.056	1.506GlnPhe: 1.506 ± 0.037	2.09GlnGly: 2.09 ± 0.045	0.714GlnHis: 0.714 ± 0.028	2.504GlnIle: 2.504 ± 0.049	2.618GlnLys: 2.618 ± 0.056	3.803GlnLeu: 3.803 ± 0.058	1.019GlnMet: 1.019 ± 0.033	1.597GlnAsn: 1.597 ± 0.036	1.14GlnPro: 1.14 ± 0.03	1.602GlnGln: 1.602 ± 0.053	1.367GlnArg: 1.367 ± 0.034	2.148GlnSer: 2.148 ± 0.046	2.064GlnThr: 2.064 ± 0.042	2.442GlnVal: 2.442 ± 0.041	0.415GlnTrp: 0.415 ± 0.019	1.132GlnTyr: 1.132 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	2.24ArgAla: 2.24 ± 0.04	0.249ArgCys: 0.249 ± 0.014	1.981ArgAsp: 1.981 ± 0.038	2.794ArgGlu: 2.794 ± 0.045	1.837ArgPhe: 1.837 ± 0.036	2.208ArgGly: 2.208 ± 0.043	0.764ArgHis: 0.764 ± 0.027	2.944ArgIle: 2.944 ± 0.052	2.975ArgLys: 2.975 ± 0.05	3.636ArgLeu: 3.636 ± 0.058	1.181ArgMet: 1.181 ± 0.031	1.898ArgAsn: 1.898 ± 0.038	1.17ArgPro: 1.17 ± 0.032	1.33ArgGln: 1.33 ± 0.029	1.592ArgArg: 1.592 ± 0.04	2.136ArgSer: 2.136 ± 0.044	2.042ArgThr: 2.042 ± 0.042	2.458ArgVal: 2.458 ± 0.049	0.361ArgTrp: 0.361 ± 0.018	1.404ArgTyr: 1.404 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	3.655SerAla: 3.655 ± 0.059	0.362SerCys: 0.362 ± 0.017	3.123SerAsp: 3.123 ± 0.048	4.058SerGlu: 4.058 ± 0.062	3.226SerPhe: 3.226 ± 0.063	4.623SerGly: 4.623 ± 0.063	1.244SerHis: 1.244 ± 0.031	5.599SerIle: 5.599 ± 0.065	3.919SerLys: 3.919 ± 0.063	6.028SerLeu: 6.028 ± 0.08	1.772SerMet: 1.772 ± 0.042	2.826SerAsn: 2.826 ± 0.052	2.047SerPro: 2.047 ± 0.042	1.86SerGln: 1.86 ± 0.04	2.176SerArg: 2.176 ± 0.046	4.046SerSer: 4.046 ± 0.066	3.332SerThr: 3.332 ± 0.061	4.196SerVal: 4.196 ± 0.064	0.681SerTrp: 0.681 ± 0.03	2.387SerTyr: 2.387 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	3.808ThrAla: 3.808 ± 0.057	0.393ThrCys: 0.393 ± 0.018	2.885ThrAsp: 2.885 ± 0.052	3.552ThrGlu: 3.552 ± 0.061	2.8ThrPhe: 2.8 ± 0.054	4.22ThrGly: 4.22 ± 0.066	1.11ThrHis: 1.11 ± 0.029	5.138ThrIle: 5.138 ± 0.065	3.613ThrLys: 3.613 ± 0.052	5.239ThrLeu: 5.239 ± 0.066	1.413ThrMet: 1.413 ± 0.038	2.783ThrAsn: 2.783 ± 0.049	2.302ThrPro: 2.302 ± 0.05	1.416ThrGln: 1.416 ± 0.032	1.839ThrArg: 1.839 ± 0.038	3.546ThrSer: 3.546 ± 0.051	3.192ThrThr: 3.192 ± 0.058	4.241ThrVal: 4.241 ± 0.067	0.56ThrTrp: 0.56 ± 0.023	2.089ThrTyr: 2.089 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	5.057ValAla: 5.057 ± 0.077	0.532ValCys: 0.532 ± 0.025	3.975ValAsp: 3.975 ± 0.057	4.907ValGlu: 4.907 ± 0.074	3.109ValPhe: 3.109 ± 0.054	5.011ValGly: 5.011 ± 0.082	1.398ValHis: 1.398 ± 0.038	5.986ValIle: 5.986 ± 0.07	4.514ValLys: 4.514 ± 0.064	6.72ValLeu: 6.72 ± 0.097	1.933ValMet: 1.933 ± 0.049	3.337ValAsn: 3.337 ± 0.061	2.739ValPro: 2.739 ± 0.051	2.247ValGln: 2.247 ± 0.045	2.421ValArg: 2.421 ± 0.046	4.611ValSer: 4.611 ± 0.068	4.228ValThr: 4.228 ± 0.062	5.258ValVal: 5.258 ± 0.077	0.646ValTrp: 0.646 ± 0.029	2.299ValTyr: 2.299 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.627TrpAla: 0.627 ± 0.026	0.061TrpCys: 0.061 ± 0.006	0.581TrpAsp: 0.581 ± 0.025	0.655TrpGlu: 0.655 ± 0.025	0.524TrpPhe: 0.524 ± 0.023	0.715TrpGly: 0.715 ± 0.026	0.2TrpHis: 0.2 ± 0.012	0.922TrpIle: 0.922 ± 0.03	0.71TrpLys: 0.71 ± 0.027	1.087TrpLeu: 1.087 ± 0.033	0.346TrpMet: 0.346 ± 0.02	0.547TrpAsn: 0.547 ± 0.021	0.28TrpPro: 0.28 ± 0.015	0.357TrpGln: 0.357 ± 0.016	0.365TrpArg: 0.365 ± 0.017	0.569TrpSer: 0.569 ± 0.028	0.53TrpThr: 0.53 ± 0.018	0.689TrpVal: 0.689 ± 0.026	0.132TrpTrp: 0.132 ± 0.012	0.365TrpTyr: 0.365 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.146TyrAla: 2.146 ± 0.04	0.302TyrCys: 0.302 ± 0.016	2.004TyrAsp: 2.004 ± 0.044	2.384TyrGlu: 2.384 ± 0.047	1.796TyrPhe: 1.796 ± 0.039	2.522TyrGly: 2.522 ± 0.044	0.863TyrHis: 0.863 ± 0.026	2.567TyrIle: 2.567 ± 0.051	2.09TyrLys: 2.09 ± 0.046	3.422TyrLeu: 3.422 ± 0.062	0.926TyrMet: 0.926 ± 0.028	1.58TyrAsn: 1.58 ± 0.037	1.455TyrPro: 1.455 ± 0.035	1.543TyrGln: 1.543 ± 0.035	1.492TyrArg: 1.492 ± 0.033	2.062TyrSer: 2.062 ± 0.044	1.906TyrThr: 1.906 ± 0.038	2.204TyrVal: 2.204 ± 0.037	0.393TyrTrp: 0.393 ± 0.017	1.455TyrTyr: 1.455 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3964 proteins (1189348 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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