Alces alces faeces associated genomovirus MP68
Average proteome isoelectric point is 7.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CI32|A0A2Z5CI32_9VIRU Replication-associated protein OS=Alces alces faeces associated genomovirus MP68 OX=2219115 PE=4 SV=1
MM1 pKa = 7.0 VRR3 pKa = 11.84 RR4 pKa = 11.84 FKK6 pKa = 11.09 LEE8 pKa = 3.71 EE9 pKa = 3.67 VSYY12 pKa = 11.61 VLLTYY17 pKa = 7.57 PTTPDD22 pKa = 3.06 GFDD25 pKa = 3.24 PQGIIDD31 pKa = 4.0 AVVGTGAVYY40 pKa = 10.28 RR41 pKa = 11.84 LGRR44 pKa = 11.84 EE45 pKa = 3.68 LHH47 pKa = 6.88 ADD49 pKa = 4.13 GKK51 pKa = 8.99 PHH53 pKa = 5.26 YY54 pKa = 10.02 HH55 pKa = 7.07 CFVQWADD62 pKa = 3.88 PYY64 pKa = 11.41 SDD66 pKa = 3.47 TDD68 pKa = 3.31 AGGTFTVGGRR78 pKa = 11.84 RR79 pKa = 11.84 PNIKK83 pKa = 10.01 KK84 pKa = 10.52 FSANPGRR91 pKa = 11.84 RR92 pKa = 11.84 WDD94 pKa = 3.78 YY95 pKa = 10.72 VGKK98 pKa = 9.62 HH99 pKa = 5.37 AGKK102 pKa = 10.38 KK103 pKa = 8.76 EE104 pKa = 3.58 GHH106 pKa = 6.6 FIIGDD111 pKa = 3.42 QCEE114 pKa = 4.28 RR115 pKa = 11.84 PSEE118 pKa = 4.14 GDD120 pKa = 2.71 VDD122 pKa = 3.88 TRR124 pKa = 11.84 SQGDD128 pKa = 2.95 IWSEE132 pKa = 3.93 IIGSGTQNEE141 pKa = 4.49 FWSRR145 pKa = 11.84 LTALAPKK152 pKa = 10.23 QLGCNFGSLKK162 pKa = 10.8 LYY164 pKa = 10.76 VDD166 pKa = 4.13 WKK168 pKa = 10.36 YY169 pKa = 11.42 KK170 pKa = 9.0 PAQEE174 pKa = 4.7 IYY176 pKa = 7.47 EE177 pKa = 4.43 TPQGTFTVPEE187 pKa = 4.16 VLQEE191 pKa = 4.08 PKK193 pKa = 10.9 GLVLFGPTRR202 pKa = 11.84 IGKK205 pKa = 6.42 TVWARR210 pKa = 11.84 SLADD214 pKa = 3.15 HH215 pKa = 7.22 AYY217 pKa = 10.45 FGGLFNLDD225 pKa = 3.99 DD226 pKa = 4.71 FSADD230 pKa = 3.03 GAQYY234 pKa = 11.09 AIFDD238 pKa = 5.15 DD239 pKa = 3.83 ISGGFSFFPSYY250 pKa = 9.2 KK251 pKa = 9.26 QWMGGQYY258 pKa = 10.39 QFTVTDD264 pKa = 3.91 KK265 pKa = 10.76 YY266 pKa = 10.28 KK267 pKa = 10.97 HH268 pKa = 5.95 KK269 pKa = 10.43 VTLRR273 pKa = 11.84 WGKK276 pKa = 7.47 PTIWLCNTDD285 pKa = 3.49 PRR287 pKa = 11.84 EE288 pKa = 3.99 DD289 pKa = 3.6 HH290 pKa = 6.54 YY291 pKa = 11.83 KK292 pKa = 10.08 PGAMPDD298 pKa = 3.87 FAWMEE303 pKa = 4.24 EE304 pKa = 3.59 NCIFYY309 pKa = 10.27 EE310 pKa = 4.09 CRR312 pKa = 11.84 DD313 pKa = 4.21 AIFHH317 pKa = 7.03 ASTEE321 pKa = 4.12
Molecular weight: 36.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.572
IPC2_protein 5.588
IPC_protein 5.614
Toseland 5.97
ProMoST 5.804
Dawson 5.842
Bjellqvist 5.881
Wikipedia 5.842
Rodwell 5.817
Grimsley 6.109
Solomon 5.842
Lehninger 5.83
Nozaki 6.097
DTASelect 6.3
Thurlkill 6.275
EMBOSS 6.237
Sillero 6.198
Patrickios 3.897
IPC_peptide 5.868
IPC2_peptide 6.186
IPC2.peptide.svr19 6.21
Protein with the highest isoelectric point:
>tr|A0A2Z5CI32|A0A2Z5CI32_9VIRU Replication-associated protein OS=Alces alces faeces associated genomovirus MP68 OX=2219115 PE=4 SV=1
MM1 pKa = 7.95 AYY3 pKa = 9.83 RR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 YY7 pKa = 7.08 TSYY10 pKa = 8.95 RR11 pKa = 11.84 AKK13 pKa = 10.43 RR14 pKa = 11.84 RR15 pKa = 11.84 TRR17 pKa = 11.84 RR18 pKa = 11.84 SARR21 pKa = 11.84 PAKK24 pKa = 10.31 SGGRR28 pKa = 11.84 KK29 pKa = 9.15 RR30 pKa = 11.84 RR31 pKa = 11.84 WTKK34 pKa = 7.47 TRR36 pKa = 11.84 KK37 pKa = 7.67 TSRR40 pKa = 11.84 PMTKK44 pKa = 10.02 KK45 pKa = 10.47 RR46 pKa = 11.84 ILNTTSTKK54 pKa = 10.42 KK55 pKa = 10.11 RR56 pKa = 11.84 DD57 pKa = 3.45 TMLTYY62 pKa = 10.65 TNSTPSAQTGGTTYY76 pKa = 11.16 ASDD79 pKa = 3.55 VPAIVVGNQGVNSAAAFLWCATARR103 pKa = 11.84 DD104 pKa = 4.14 NTTSNANGKK113 pKa = 7.7 ATKK116 pKa = 10.18 FDD118 pKa = 3.25 MSTRR122 pKa = 11.84 TSSTPYY128 pKa = 9.9 MIGLKK133 pKa = 10.11 EE134 pKa = 4.23 SIEE137 pKa = 4.11 IQVNTGMPWQWRR149 pKa = 11.84 RR150 pKa = 11.84 ICFTVKK156 pKa = 10.43 GSLGLTQTSSFGLSTEE172 pKa = 3.97 TSAGYY177 pKa = 10.48 VRR179 pKa = 11.84 VLNQLPGDD187 pKa = 4.05 PGGGQAYY194 pKa = 9.57 ILYY197 pKa = 9.2 EE198 pKa = 3.86 RR199 pKa = 11.84 LFRR202 pKa = 11.84 GQNTTDD208 pKa = 2.94 WNDD211 pKa = 2.68 VMTAKK216 pKa = 10.32 VDD218 pKa = 3.63 TSRR221 pKa = 11.84 VTLKK225 pKa = 10.59 YY226 pKa = 10.92 DD227 pKa = 3.15 KK228 pKa = 10.17 TYY230 pKa = 11.39 SLASGNEE237 pKa = 3.47 NGFIRR242 pKa = 11.84 KK243 pKa = 8.31 YY244 pKa = 10.58 NRR246 pKa = 11.84 WHH248 pKa = 7.28 DD249 pKa = 3.45 MHH251 pKa = 6.53 KK252 pKa = 9.54 TLVYY256 pKa = 10.84 NDD258 pKa = 4.72 DD259 pKa = 3.9 EE260 pKa = 5.81 LGGSTNPQSFSTTGKK275 pKa = 10.73 GGMGDD280 pKa = 3.94 YY281 pKa = 10.85 YY282 pKa = 11.52 VVDD285 pKa = 4.32 LFRR288 pKa = 11.84 ARR290 pKa = 11.84 TGSSVNDD297 pKa = 3.33 QLSFRR302 pKa = 11.84 PEE304 pKa = 3.6 TTLYY308 pKa = 7.96 WHH310 pKa = 6.68 EE311 pKa = 4.11 RR312 pKa = 3.51
Molecular weight: 35.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.78
IPC_protein 10.365
Toseland 10.379
ProMoST 10.145
Dawson 10.555
Bjellqvist 10.262
Wikipedia 10.76
Rodwell 10.862
Grimsley 10.628
Solomon 10.599
Lehninger 10.555
Nozaki 10.35
DTASelect 10.262
Thurlkill 10.409
EMBOSS 10.774
Sillero 10.467
Patrickios 10.482
IPC_peptide 10.599
IPC2_peptide 9.092
IPC2.peptide.svr19 8.669
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
633
312
321
316.5
35.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.161 ± 0.049
1.264 ± 0.431
6.161 ± 0.937
3.949 ± 0.959
4.739 ± 1.283
9.637 ± 0.68
1.896 ± 0.646
3.633 ± 0.74
6.003 ± 0.06
5.687 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.054 ± 0.575
3.791 ± 1.147
4.581 ± 0.952
3.633 ± 0.296
7.267 ± 1.625
6.793 ± 1.509
9.953 ± 2.428
5.055 ± 0.171
2.686 ± 0.306
5.055 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here