Amino acid dipepetide frequency for Prochlorococcus phage P-SSP3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.813AlaAla: 8.813 ± 0.967
0.715AlaCys: 0.715 ± 0.255
6.272AlaAsp: 6.272 ± 1.11
3.573AlaGlu: 3.573 ± 0.492
3.017AlaPhe: 3.017 ± 0.484
5.875AlaGly: 5.875 ± 0.719
1.27AlaHis: 1.27 ± 0.337
4.129AlaIle: 4.129 ± 0.432
5.558AlaLys: 5.558 ± 0.984
5.796AlaLeu: 5.796 ± 0.521
2.461AlaMet: 2.461 ± 0.517
4.526AlaAsn: 4.526 ± 0.648
2.382AlaPro: 2.382 ± 0.433
3.255AlaGln: 3.255 ± 0.652
3.811AlaArg: 3.811 ± 0.67
6.034AlaSer: 6.034 ± 0.743
5.32AlaThr: 5.32 ± 0.716
5.478AlaVal: 5.478 ± 0.67
0.715AlaTrp: 0.715 ± 0.196
2.779AlaTyr: 2.779 ± 0.404
0.0AlaXaa: 0.0 ± 0.0
Cys
0.635CysAla: 0.635 ± 0.216
0.079CysCys: 0.079 ± 0.086
0.556CysAsp: 0.556 ± 0.208
0.476CysGlu: 0.476 ± 0.217
0.238CysPhe: 0.238 ± 0.134
0.397CysGly: 0.397 ± 0.172
0.079CysHis: 0.079 ± 0.083
0.238CysIle: 0.238 ± 0.135
0.715CysLys: 0.715 ± 0.242
0.794CysLeu: 0.794 ± 0.256
0.159CysMet: 0.159 ± 0.114
0.397CysAsn: 0.397 ± 0.246
0.397CysPro: 0.397 ± 0.234
0.238CysGln: 0.238 ± 0.14
0.556CysArg: 0.556 ± 0.199
0.953CysSer: 0.953 ± 0.31
1.112CysThr: 1.112 ± 0.323
0.873CysVal: 0.873 ± 0.318
0.0CysTrp: 0.0 ± 0.0
0.318CysTyr: 0.318 ± 0.123
0.0CysXaa: 0.0 ± 0.0
Asp
5.558AspAla: 5.558 ± 0.604
0.476AspCys: 0.476 ± 0.187
5.558AspAsp: 5.558 ± 0.58
3.652AspGlu: 3.652 ± 0.573
2.699AspPhe: 2.699 ± 0.342
5.717AspGly: 5.717 ± 0.946
0.873AspHis: 0.873 ± 0.275
4.287AspIle: 4.287 ± 0.575
3.414AspLys: 3.414 ± 0.735
6.749AspLeu: 6.749 ± 0.94
0.873AspMet: 0.873 ± 0.273
3.255AspAsn: 3.255 ± 0.489
3.017AspPro: 3.017 ± 0.629
2.699AspGln: 2.699 ± 0.445
2.62AspArg: 2.62 ± 0.488
4.367AspSer: 4.367 ± 0.644
4.526AspThr: 4.526 ± 0.482
4.129AspVal: 4.129 ± 0.563
0.953AspTrp: 0.953 ± 0.222
2.064AspTyr: 2.064 ± 0.336
0.0AspXaa: 0.0 ± 0.0
Glu
4.764GluAla: 4.764 ± 0.676
0.397GluCys: 0.397 ± 0.271
4.208GluAsp: 4.208 ± 0.668
5.32GluGlu: 5.32 ± 0.894
1.906GluPhe: 1.906 ± 0.465
3.97GluGly: 3.97 ± 0.516
1.429GluHis: 1.429 ± 0.428
4.129GluIle: 4.129 ± 0.68
3.414GluLys: 3.414 ± 0.683
5.478GluLeu: 5.478 ± 0.766
1.747GluMet: 1.747 ± 0.406
2.779GluAsn: 2.779 ± 0.535
1.985GluPro: 1.985 ± 0.434
2.223GluGln: 2.223 ± 0.402
3.096GluArg: 3.096 ± 0.473
2.62GluSer: 2.62 ± 0.434
3.97GluThr: 3.97 ± 0.54
3.573GluVal: 3.573 ± 0.533
0.476GluTrp: 0.476 ± 0.23
2.144GluTyr: 2.144 ± 0.537
0.0GluXaa: 0.0 ± 0.0
Phe
3.017PheAla: 3.017 ± 0.578
0.318PheCys: 0.318 ± 0.167
2.461PheAsp: 2.461 ± 0.479
1.906PheGlu: 1.906 ± 0.451
1.588PhePhe: 1.588 ± 0.401
3.176PheGly: 3.176 ± 0.599
0.635PheHis: 0.635 ± 0.283
2.064PheIle: 2.064 ± 0.335
2.699PheLys: 2.699 ± 0.555
2.62PheLeu: 2.62 ± 0.524
1.509PheMet: 1.509 ± 0.312
2.779PheAsn: 2.779 ± 0.474
1.27PhePro: 1.27 ± 0.379
1.588PheGln: 1.588 ± 0.421
1.032PheArg: 1.032 ± 0.28
2.541PheSer: 2.541 ± 0.431
2.541PheThr: 2.541 ± 0.526
3.017PheVal: 3.017 ± 0.448
0.238PheTrp: 0.238 ± 0.131
1.112PheTyr: 1.112 ± 0.279
0.0PheXaa: 0.0 ± 0.0
Gly
5.478GlyAla: 5.478 ± 0.685
0.318GlyCys: 0.318 ± 0.144
5.637GlyAsp: 5.637 ± 0.558
3.732GlyGlu: 3.732 ± 0.492
3.017GlyPhe: 3.017 ± 0.45
4.129GlyGly: 4.129 ± 0.635
0.873GlyHis: 0.873 ± 0.272
4.049GlyIle: 4.049 ± 0.606
3.493GlyLys: 3.493 ± 0.339
4.446GlyLeu: 4.446 ± 0.496
1.429GlyMet: 1.429 ± 0.392
4.684GlyAsn: 4.684 ± 0.753
1.429GlyPro: 1.429 ± 0.355
2.699GlyGln: 2.699 ± 0.39
3.414GlyArg: 3.414 ± 0.563
5.24GlySer: 5.24 ± 1.162
6.431GlyThr: 6.431 ± 1.074
5.399GlyVal: 5.399 ± 0.821
0.873GlyTrp: 0.873 ± 0.316
2.938GlyTyr: 2.938 ± 0.48
0.0GlyXaa: 0.0 ± 0.0
His
0.476HisAla: 0.476 ± 0.21
0.318HisCys: 0.318 ± 0.253
1.191HisAsp: 1.191 ± 0.344
0.794HisGlu: 0.794 ± 0.264
0.715HisPhe: 0.715 ± 0.279
1.429HisGly: 1.429 ± 0.432
0.476HisHis: 0.476 ± 0.26
1.112HisIle: 1.112 ± 0.442
0.873HisLys: 0.873 ± 0.333
1.509HisLeu: 1.509 ± 0.325
0.159HisMet: 0.159 ± 0.106
0.953HisAsn: 0.953 ± 0.301
0.556HisPro: 0.556 ± 0.216
0.635HisGln: 0.635 ± 0.249
0.635HisArg: 0.635 ± 0.222
0.794HisSer: 0.794 ± 0.226
1.429HisThr: 1.429 ± 0.383
0.794HisVal: 0.794 ± 0.253
0.397HisTrp: 0.397 ± 0.171
0.953HisTyr: 0.953 ± 0.291
0.0HisXaa: 0.0 ± 0.0
Ile
5.161IleAla: 5.161 ± 1.093
0.476IleCys: 0.476 ± 0.199
5.399IleAsp: 5.399 ± 0.573
3.096IleGlu: 3.096 ± 0.389
2.064IlePhe: 2.064 ± 0.332
2.938IleGly: 2.938 ± 0.409
1.35IleHis: 1.35 ± 0.347
3.017IleIle: 3.017 ± 0.448
4.605IleLys: 4.605 ± 0.696
3.811IleLeu: 3.811 ± 0.695
1.27IleMet: 1.27 ± 0.3
3.573IleAsn: 3.573 ± 0.51
2.699IlePro: 2.699 ± 0.522
3.017IleGln: 3.017 ± 0.514
2.541IleArg: 2.541 ± 0.438
4.287IleSer: 4.287 ± 0.549
3.89IleThr: 3.89 ± 0.675
2.779IleVal: 2.779 ± 0.654
0.635IleTrp: 0.635 ± 0.269
1.588IleTyr: 1.588 ± 0.409
0.0IleXaa: 0.0 ± 0.0
Lys
5.24LysAla: 5.24 ± 0.779
0.397LysCys: 0.397 ± 0.25
3.97LysAsp: 3.97 ± 0.528
3.89LysGlu: 3.89 ± 0.621
3.176LysPhe: 3.176 ± 0.569
4.049LysGly: 4.049 ± 0.814
1.191LysHis: 1.191 ± 0.333
4.526LysIle: 4.526 ± 1.317
5.161LysLys: 5.161 ± 0.792
6.749LysLeu: 6.749 ± 0.685
1.429LysMet: 1.429 ± 0.462
3.017LysAsn: 3.017 ± 0.474
2.303LysPro: 2.303 ± 0.429
2.938LysGln: 2.938 ± 0.518
3.335LysArg: 3.335 ± 0.608
3.573LysSer: 3.573 ± 0.538
3.493LysThr: 3.493 ± 0.496
3.335LysVal: 3.335 ± 0.549
0.635LysTrp: 0.635 ± 0.223
3.017LysTyr: 3.017 ± 0.416
0.0LysXaa: 0.0 ± 0.0
Leu
5.637LeuAla: 5.637 ± 0.609
1.112LeuCys: 1.112 ± 0.343
5.717LeuAsp: 5.717 ± 0.554
4.287LeuGlu: 4.287 ± 0.769
2.461LeuPhe: 2.461 ± 0.471
5.399LeuGly: 5.399 ± 0.736
1.588LeuHis: 1.588 ± 0.408
4.843LeuIle: 4.843 ± 0.634
6.114LeuLys: 6.114 ± 0.711
5.955LeuLeu: 5.955 ± 0.69
1.747LeuMet: 1.747 ± 0.452
3.97LeuAsn: 3.97 ± 0.541
4.049LeuPro: 4.049 ± 0.61
4.049LeuGln: 4.049 ± 0.851
3.255LeuArg: 3.255 ± 0.688
4.605LeuSer: 4.605 ± 0.542
5.478LeuThr: 5.478 ± 0.554
5.081LeuVal: 5.081 ± 0.594
0.794LeuTrp: 0.794 ± 0.246
3.414LeuTyr: 3.414 ± 0.569
0.0LeuXaa: 0.0 ± 0.0
Met
2.064MetAla: 2.064 ± 0.462
0.397MetCys: 0.397 ± 0.195
1.35MetAsp: 1.35 ± 0.307
1.588MetGlu: 1.588 ± 0.465
0.953MetPhe: 0.953 ± 0.268
1.509MetGly: 1.509 ± 0.584
0.397MetHis: 0.397 ± 0.274
0.953MetIle: 0.953 ± 0.219
2.144MetLys: 2.144 ± 0.44
1.747MetLeu: 1.747 ± 0.4
0.079MetMet: 0.079 ± 0.085
1.191MetAsn: 1.191 ± 0.318
0.873MetPro: 0.873 ± 0.223
0.953MetGln: 0.953 ± 0.25
0.873MetArg: 0.873 ± 0.25
1.906MetSer: 1.906 ± 0.483
1.509MetThr: 1.509 ± 0.326
0.953MetVal: 0.953 ± 0.261
0.159MetTrp: 0.159 ± 0.089
0.715MetTyr: 0.715 ± 0.212
0.0MetXaa: 0.0 ± 0.0
Asn
5.24AsnAla: 5.24 ± 0.922
0.556AsnCys: 0.556 ± 0.199
2.303AsnAsp: 2.303 ± 0.459
2.699AsnGlu: 2.699 ± 0.347
2.144AsnPhe: 2.144 ± 0.32
5.399AsnGly: 5.399 ± 0.733
0.873AsnHis: 0.873 ± 0.209
3.176AsnIle: 3.176 ± 0.474
2.858AsnLys: 2.858 ± 0.701
4.526AsnLeu: 4.526 ± 0.587
0.635AsnMet: 0.635 ± 0.192
3.811AsnAsn: 3.811 ± 0.584
3.176AsnPro: 3.176 ± 0.462
2.064AsnGln: 2.064 ± 0.363
3.652AsnArg: 3.652 ± 0.656
4.287AsnSer: 4.287 ± 0.914
3.493AsnThr: 3.493 ± 0.572
3.335AsnVal: 3.335 ± 0.542
0.715AsnTrp: 0.715 ± 0.258
1.985AsnTyr: 1.985 ± 0.323
0.0AsnXaa: 0.0 ± 0.0
Pro
2.382ProAla: 2.382 ± 0.407
0.318ProCys: 0.318 ± 0.151
2.938ProAsp: 2.938 ± 0.562
4.049ProGlu: 4.049 ± 0.62
1.588ProPhe: 1.588 ± 0.351
2.064ProGly: 2.064 ± 0.424
0.318ProHis: 0.318 ± 0.151
2.541ProIle: 2.541 ± 0.574
1.985ProLys: 1.985 ± 0.452
2.382ProLeu: 2.382 ± 0.445
0.794ProMet: 0.794 ± 0.295
2.382ProAsn: 2.382 ± 0.32
2.461ProPro: 2.461 ± 0.791
1.667ProGln: 1.667 ± 0.371
0.794ProArg: 0.794 ± 0.26
3.096ProSer: 3.096 ± 0.416
2.303ProThr: 2.303 ± 0.414
3.017ProVal: 3.017 ± 0.549
1.112ProTrp: 1.112 ± 0.285
1.747ProTyr: 1.747 ± 0.398
0.0ProXaa: 0.0 ± 0.0
Gln
4.208GlnAla: 4.208 ± 0.974
0.476GlnCys: 0.476 ± 0.187
1.588GlnAsp: 1.588 ± 0.421
2.461GlnGlu: 2.461 ± 0.415
1.588GlnPhe: 1.588 ± 0.333
3.096GlnGly: 3.096 ± 0.433
0.953GlnHis: 0.953 ± 0.279
3.176GlnIle: 3.176 ± 0.54
2.382GlnLys: 2.382 ± 0.418
4.526GlnLeu: 4.526 ± 0.616
1.191GlnMet: 1.191 ± 0.263
1.667GlnAsn: 1.667 ± 0.398
1.27GlnPro: 1.27 ± 0.31
1.985GlnGln: 1.985 ± 0.385
1.667GlnArg: 1.667 ± 0.302
2.62GlnSer: 2.62 ± 0.355
2.779GlnThr: 2.779 ± 0.442
2.699GlnVal: 2.699 ± 0.399
0.159GlnTrp: 0.159 ± 0.119
1.588GlnTyr: 1.588 ± 0.292
0.0GlnXaa: 0.0 ± 0.0
Arg
3.017ArgAla: 3.017 ± 0.491
0.476ArgCys: 0.476 ± 0.239
2.62ArgAsp: 2.62 ± 0.428
2.699ArgGlu: 2.699 ± 0.595
2.144ArgPhe: 2.144 ± 0.281
2.223ArgGly: 2.223 ± 0.419
0.476ArgHis: 0.476 ± 0.248
2.223ArgIle: 2.223 ± 0.454
3.017ArgLys: 3.017 ± 0.669
4.764ArgLeu: 4.764 ± 0.649
1.667ArgMet: 1.667 ± 0.394
2.858ArgAsn: 2.858 ± 0.391
1.747ArgPro: 1.747 ± 0.478
3.255ArgGln: 3.255 ± 0.553
2.144ArgArg: 2.144 ± 0.477
2.303ArgSer: 2.303 ± 0.41
2.541ArgThr: 2.541 ± 0.414
2.541ArgVal: 2.541 ± 0.473
0.794ArgTrp: 0.794 ± 0.201
1.826ArgTyr: 1.826 ± 0.384
0.0ArgXaa: 0.0 ± 0.0
Ser
5.637SerAla: 5.637 ± 0.638
0.397SerCys: 0.397 ± 0.21
5.32SerAsp: 5.32 ± 0.904
3.811SerGlu: 3.811 ± 0.71
1.985SerPhe: 1.985 ± 0.361
6.908SerGly: 6.908 ± 1.072
0.715SerHis: 0.715 ± 0.245
3.652SerIle: 3.652 ± 0.642
3.335SerLys: 3.335 ± 0.419
4.208SerLeu: 4.208 ± 0.493
1.509SerMet: 1.509 ± 0.35
4.843SerAsn: 4.843 ± 0.658
3.335SerPro: 3.335 ± 0.541
2.064SerGln: 2.064 ± 0.341
3.335SerArg: 3.335 ± 0.6
5.478SerSer: 5.478 ± 1.047
5.002SerThr: 5.002 ± 0.583
3.414SerVal: 3.414 ± 0.405
0.873SerTrp: 0.873 ± 0.276
1.906SerTyr: 1.906 ± 0.381
0.0SerXaa: 0.0 ± 0.0
Thr
5.32ThrAla: 5.32 ± 0.802
0.715ThrCys: 0.715 ± 0.318
3.493ThrAsp: 3.493 ± 0.521
4.208ThrGlu: 4.208 ± 0.511
2.62ThrPhe: 2.62 ± 0.421
4.764ThrGly: 4.764 ± 1.053
0.715ThrHis: 0.715 ± 0.268
4.287ThrIle: 4.287 ± 0.658
5.002ThrLys: 5.002 ± 0.681
5.637ThrLeu: 5.637 ± 0.787
1.27ThrMet: 1.27 ± 0.314
4.526ThrAsn: 4.526 ± 0.763
3.573ThrPro: 3.573 ± 0.664
2.938ThrGln: 2.938 ± 0.408
2.461ThrArg: 2.461 ± 0.529
5.081ThrSer: 5.081 ± 0.957
7.463ThrThr: 7.463 ± 1.561
3.811ThrVal: 3.811 ± 0.629
1.112ThrTrp: 1.112 ± 0.359
2.62ThrTyr: 2.62 ± 0.487
0.0ThrXaa: 0.0 ± 0.0
Val
5.558ValAla: 5.558 ± 0.665
0.635ValCys: 0.635 ± 0.211
4.049ValAsp: 4.049 ± 0.63
4.129ValGlu: 4.129 ± 0.576
2.62ValPhe: 2.62 ± 0.521
3.97ValGly: 3.97 ± 0.576
0.873ValHis: 0.873 ± 0.267
3.096ValIle: 3.096 ± 0.551
4.684ValLys: 4.684 ± 0.593
3.414ValLeu: 3.414 ± 0.516
1.191ValMet: 1.191 ± 0.255
3.176ValAsn: 3.176 ± 0.549
1.985ValPro: 1.985 ± 0.436
2.303ValGln: 2.303 ± 0.471
2.938ValArg: 2.938 ± 0.444
4.208ValSer: 4.208 ± 0.516
5.24ValThr: 5.24 ± 1.021
3.97ValVal: 3.97 ± 0.572
0.556ValTrp: 0.556 ± 0.233
2.223ValTyr: 2.223 ± 0.322
0.0ValXaa: 0.0 ± 0.0
Trp
1.032TrpAla: 1.032 ± 0.318
0.159TrpCys: 0.159 ± 0.093
0.556TrpAsp: 0.556 ± 0.215
0.873TrpGlu: 0.873 ± 0.342
0.238TrpPhe: 0.238 ± 0.115
0.556TrpGly: 0.556 ± 0.269
0.476TrpHis: 0.476 ± 0.206
0.635TrpIle: 0.635 ± 0.213
0.873TrpLys: 0.873 ± 0.258
1.509TrpLeu: 1.509 ± 0.359
0.397TrpMet: 0.397 ± 0.183
0.794TrpAsn: 0.794 ± 0.253
0.318TrpPro: 0.318 ± 0.136
0.159TrpGln: 0.159 ± 0.108
1.032TrpArg: 1.032 ± 0.33
0.715TrpSer: 0.715 ± 0.283
0.873TrpThr: 0.873 ± 0.274
0.318TrpVal: 0.318 ± 0.141
0.079TrpTrp: 0.079 ± 0.08
0.079TrpTyr: 0.079 ± 0.084
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.541TyrAla: 2.541 ± 0.485
0.476TyrCys: 0.476 ± 0.23
2.303TyrAsp: 2.303 ± 0.447
2.382TyrGlu: 2.382 ± 0.486
1.429TyrPhe: 1.429 ± 0.336
2.144TyrGly: 2.144 ± 0.358
0.635TyrHis: 0.635 ± 0.228
2.064TyrIle: 2.064 ± 0.398
3.096TyrLys: 3.096 ± 0.757
2.938TyrLeu: 2.938 ± 0.457
0.715TyrMet: 0.715 ± 0.277
1.747TyrAsn: 1.747 ± 0.374
1.112TyrPro: 1.112 ± 0.325
1.35TyrGln: 1.35 ± 0.276
2.144TyrArg: 2.144 ± 0.406
3.017TyrSer: 3.017 ± 0.516
2.223TyrThr: 2.223 ± 0.361
2.303TyrVal: 2.303 ± 0.476
0.397TyrTrp: 0.397 ± 0.168
1.27TyrTyr: 1.27 ± 0.323
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (12596 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski