Beihai weivirus-like virus 13
Average proteome isoelectric point is 7.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KL24|A0A1L3KL24_9VIRU Uncharacterized protein OS=Beihai weivirus-like virus 13 OX=1922742 PE=4 SV=1
MM1 pKa = 7.4 AVVSFRR7 pKa = 11.84 PPSRR11 pKa = 11.84 RR12 pKa = 11.84 NDD14 pKa = 3.17 LCYY17 pKa = 10.43 EE18 pKa = 4.09 CHH20 pKa = 6.38 SWMLQHH26 pKa = 6.55 LTYY29 pKa = 10.58 EE30 pKa = 4.45 HH31 pKa = 6.92 ASGRR35 pKa = 11.84 ADD37 pKa = 3.2 VSDD40 pKa = 3.43 TFLYY44 pKa = 10.54 EE45 pKa = 5.1 LEE47 pKa = 4.33 LQVARR52 pKa = 11.84 CGRR55 pKa = 11.84 HH56 pKa = 5.52 VEE58 pKa = 4.31 TCAQRR63 pKa = 11.84 IRR65 pKa = 11.84 DD66 pKa = 3.41 RR67 pKa = 11.84 LGRR70 pKa = 11.84 IKK72 pKa = 10.5 HH73 pKa = 5.49 ALCGQFHH80 pKa = 6.48 GVGVHH85 pKa = 5.93 HH86 pKa = 7.26 VYY88 pKa = 9.12 LTRR91 pKa = 11.84 GEE93 pKa = 3.96 VDD95 pKa = 2.47 IMRR98 pKa = 11.84 RR99 pKa = 11.84 SGGAALYY106 pKa = 10.55 SGAPTTTADD115 pKa = 3.86 SNLHH119 pKa = 5.44 FATYY123 pKa = 10.7 RR124 pKa = 11.84 LDD126 pKa = 3.46 KK127 pKa = 10.66 KK128 pKa = 9.56 EE129 pKa = 3.87 RR130 pKa = 11.84 RR131 pKa = 11.84 GDD133 pKa = 4.12 PISCATIDD141 pKa = 3.6 QLFGYY146 pKa = 9.73 LAATIQQGRR155 pKa = 11.84 VANRR159 pKa = 11.84 HH160 pKa = 5.46 CCCLHH165 pKa = 5.34 TAGLGLRR172 pKa = 11.84 NLRR175 pKa = 11.84 PVKK178 pKa = 10.26 RR179 pKa = 11.84 MPEE182 pKa = 4.11 DD183 pKa = 3.41 EE184 pKa = 4.7 PEE186 pKa = 4.26 PPKK189 pKa = 10.96 SDD191 pKa = 3.43 DD192 pKa = 3.73 GKK194 pKa = 10.42 DD195 pKa = 3.57 GKK197 pKa = 11.18 VEE199 pKa = 4.29 GPPEE203 pKa = 4.82 DD204 pKa = 5.43 DD205 pKa = 4.36 PPQVDD210 pKa = 3.79 CHH212 pKa = 6.11 TCHH215 pKa = 6.82 SGLAGIQLIHH225 pKa = 7.18 DD226 pKa = 4.1 VNEE229 pKa = 4.5 GPEE232 pKa = 4.32 TIPLAVRR239 pKa = 11.84 YY240 pKa = 9.05 LPQIEE245 pKa = 4.96 EE246 pKa = 3.94 RR247 pKa = 11.84 LFWLNSQKK255 pKa = 10.66 NVEE258 pKa = 4.1 RR259 pKa = 11.84 AIKK262 pKa = 9.85 EE263 pKa = 4.18 RR264 pKa = 11.84 IEE266 pKa = 4.18 EE267 pKa = 3.92 PHH269 pKa = 6.83 VPVTLSQEE277 pKa = 4.16 EE278 pKa = 4.23 KK279 pKa = 10.65 DD280 pKa = 3.82 DD281 pKa = 3.98 LRR283 pKa = 11.84 AVTQVMIDD291 pKa = 3.3 QLKK294 pKa = 9.97 ADD296 pKa = 4.47 KK297 pKa = 11.16 GLIDD301 pKa = 6.26 KK302 pKa = 10.53 IIQDD306 pKa = 3.55 KK307 pKa = 10.98 LGIYY311 pKa = 8.68 GWKK314 pKa = 8.34 SKK316 pKa = 9.54 KK317 pKa = 8.6 WAPARR322 pKa = 11.84 AEE324 pKa = 4.01 KK325 pKa = 10.63 ALSEE329 pKa = 4.1 LRR331 pKa = 11.84 ATFAPKK337 pKa = 9.95 FRR339 pKa = 11.84 FDD341 pKa = 3.89 AGIKK345 pKa = 10.11 LEE347 pKa = 3.92 PSKK350 pKa = 10.66 RR351 pKa = 11.84 GKK353 pKa = 10.44 APRR356 pKa = 11.84 LLIADD361 pKa = 4.13 GDD363 pKa = 4.11 RR364 pKa = 11.84 GQVMSWVLIGTLEE377 pKa = 3.68 AWLFKK382 pKa = 10.1 RR383 pKa = 11.84 WRR385 pKa = 11.84 HH386 pKa = 5.12 RR387 pKa = 11.84 SIKK390 pKa = 10.29 GLPKK394 pKa = 9.24 TEE396 pKa = 3.5 AMQRR400 pKa = 11.84 VCQSLRR406 pKa = 11.84 QKK408 pKa = 10.8 DD409 pKa = 3.86 PRR411 pKa = 11.84 AGPDD415 pKa = 3.25 TPDD418 pKa = 3.03 IPVSIVEE425 pKa = 4.31 NDD427 pKa = 3.07 GSAWDD432 pKa = 3.56 ACMSEE437 pKa = 4.35 TLRR440 pKa = 11.84 SLTEE444 pKa = 3.95 NPVMEE449 pKa = 4.94 AVAEE453 pKa = 4.1 MVEE456 pKa = 4.4 QYY458 pKa = 10.91 FLVEE462 pKa = 4.61 GPPEE466 pKa = 4.53 FIDD469 pKa = 4.6 ARR471 pKa = 11.84 VASNRR476 pKa = 11.84 LTKK479 pKa = 10.6 LQLGFRR485 pKa = 11.84 KK486 pKa = 10.22 GKK488 pKa = 10.83 GGDD491 pKa = 3.78 DD492 pKa = 3.54 YY493 pKa = 11.87 VGKK496 pKa = 10.62 CDD498 pKa = 4.75 LPKK501 pKa = 10.85 GKK503 pKa = 9.77 AWQTIISAIRR513 pKa = 11.84 RR514 pKa = 11.84 SGCRR518 pKa = 11.84 GTSCLNFLANMICWAWVIGGRR539 pKa = 11.84 DD540 pKa = 3.15 ACKK543 pKa = 10.03 LVRR546 pKa = 11.84 PQGARR551 pKa = 11.84 VTCVDD556 pKa = 3.27 GVVRR560 pKa = 11.84 FVKK563 pKa = 10.01 MVFEE567 pKa = 5.14 GDD569 pKa = 3.5 DD570 pKa = 4.02 SILSFVGLNTAGQAADD586 pKa = 3.46 QQLSRR591 pKa = 11.84 EE592 pKa = 4.27 YY593 pKa = 10.5 IHH595 pKa = 6.99 TCTQRR600 pKa = 11.84 WTKK603 pKa = 10.44 LGHH606 pKa = 6.02 RR607 pKa = 11.84 PKK609 pKa = 10.59 LYY611 pKa = 9.05 WRR613 pKa = 11.84 AIGAVAEE620 pKa = 4.15 FTGWHH625 pKa = 6.16 FSVSDD630 pKa = 3.36 VGIGSDD636 pKa = 4.01 CAAPDD641 pKa = 4.74 LIRR644 pKa = 11.84 NLTNMAYY651 pKa = 10.14 SINAAAINATAAGDD665 pKa = 3.83 RR666 pKa = 11.84 KK667 pKa = 10.9 ALMSAVAPGVIARR680 pKa = 11.84 LYY682 pKa = 10.21 PMAEE686 pKa = 4.33 KK687 pKa = 10.61 FPMLCKK693 pKa = 10.51 LLYY696 pKa = 10.58 GQFAHH701 pKa = 7.18 HH702 pKa = 6.78 LRR704 pKa = 11.84 DD705 pKa = 3.47 TTTTDD710 pKa = 3.09 LTRR713 pKa = 11.84 DD714 pKa = 3.46 EE715 pKa = 4.96 IYY717 pKa = 10.93 ALEE720 pKa = 4.77 LEE722 pKa = 4.74 PEE724 pKa = 4.07 DD725 pKa = 5.15 FGFKK729 pKa = 10.3 EE730 pKa = 3.89 SDD732 pKa = 3.54 YY733 pKa = 10.7 NTDD736 pKa = 2.69 MDD738 pKa = 5.19 LNIEE742 pKa = 4.12 RR743 pKa = 11.84 ATQRR747 pKa = 11.84 FQPILEE753 pKa = 4.21 RR754 pKa = 11.84 FEE756 pKa = 3.98 MAIGRR761 pKa = 11.84 GNEE764 pKa = 3.79 QEE766 pKa = 4.2 EE767 pKa = 3.95 ADD769 pKa = 3.82 LAVRR773 pKa = 11.84 LGLVPDD779 pKa = 3.39 NDD781 pKa = 3.71 KK782 pKa = 11.62 YY783 pKa = 11.8 YY784 pKa = 11.08 DD785 pKa = 3.94 LLDD788 pKa = 5.04 AIEE791 pKa = 4.29 GGYY794 pKa = 10.0 RR795 pKa = 11.84 VGADD799 pKa = 2.91 SAAFARR805 pKa = 11.84 SVEE808 pKa = 4.38 AIRR811 pKa = 11.84 EE812 pKa = 4.32 GG813 pKa = 3.49
Molecular weight: 90.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.272
IPC2_protein 6.287
IPC_protein 6.338
Toseland 6.465
ProMoST 6.781
Dawson 6.722
Bjellqvist 6.737
Wikipedia 6.722
Rodwell 6.722
Grimsley 6.561
Solomon 6.722
Lehninger 6.722
Nozaki 7.044
DTASelect 7.132
Thurlkill 7.176
EMBOSS 7.146
Sillero 7.132
Patrickios 4.355
IPC_peptide 6.737
IPC2_peptide 6.927
IPC2.peptide.svr19 6.808
Protein with the highest isoelectric point:
>tr|A0A1L3KL67|A0A1L3KL67_9VIRU Uncharacterized protein OS=Beihai weivirus-like virus 13 OX=1922742 PE=4 SV=1
MM1 pKa = 7.15 VKK3 pKa = 10.34 KK4 pKa = 10.23 FQLKK8 pKa = 9.67 KK9 pKa = 10.53 KK10 pKa = 10.41 RR11 pKa = 11.84 VGQKK15 pKa = 9.92 AATRR19 pKa = 11.84 SKK21 pKa = 9.67 ATRR24 pKa = 11.84 VLAQGAGAVPRR35 pKa = 11.84 KK36 pKa = 10.22 AFGAGKK42 pKa = 9.96 SLARR46 pKa = 11.84 KK47 pKa = 8.09 GWGMSMRR54 pKa = 11.84 RR55 pKa = 11.84 RR56 pKa = 11.84 MMEE59 pKa = 3.83 GLNARR64 pKa = 11.84 LPMHH68 pKa = 7.03 LGLPRR73 pKa = 11.84 PVGPYY78 pKa = 9.41 QVIRR82 pKa = 11.84 TTTLHH87 pKa = 4.95 TTSAAVVIFAPLMRR101 pKa = 11.84 DD102 pKa = 3.04 ASTTSNGHH110 pKa = 5.24 PVWYY114 pKa = 9.04 HH115 pKa = 5.18 ACGLEE120 pKa = 4.15 DD121 pKa = 4.43 AAPGPINGGAGTRR134 pKa = 11.84 MISMPLDD141 pKa = 4.56 GIGSAADD148 pKa = 3.38 IVPAALTVQVMNKK161 pKa = 10.18 ASLQQAEE168 pKa = 4.91 GLFTMARR175 pKa = 11.84 VSQQLALGGEE185 pKa = 4.15 TRR187 pKa = 11.84 TWEE190 pKa = 4.33 DD191 pKa = 3.42 FTHH194 pKa = 6.58 EE195 pKa = 4.25 FNSYY199 pKa = 9.86 FKK201 pKa = 10.69 PRR203 pKa = 11.84 LLTGAKK209 pKa = 9.46 LALRR213 pKa = 11.84 GVTCNAVPINMNEE226 pKa = 3.9 YY227 pKa = 10.22 SDD229 pKa = 4.15 FRR231 pKa = 11.84 PGIPAISPAFGWYY244 pKa = 8.92 TEE246 pKa = 4.13 VAPAALSPIVFTQTGTPVDD265 pKa = 3.19 ISFLVTIEE273 pKa = 3.47 WRR275 pKa = 11.84 VRR277 pKa = 11.84 FDD279 pKa = 3.6 PFHH282 pKa = 6.87 PAAASHH288 pKa = 6.21 TFHH291 pKa = 7.59 PSTPDD296 pKa = 3.48 GVWNSIIGAASAAGHH311 pKa = 5.69 GVVDD315 pKa = 4.23 IADD318 pKa = 3.75 EE319 pKa = 4.18 VADD322 pKa = 3.86 VGFDD326 pKa = 3.29 AAAVAGVAAALL337 pKa = 3.79
Molecular weight: 35.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.091
IPC2_protein 9.121
IPC_protein 9.165
Toseland 9.984
ProMoST 9.619
Dawson 10.16
Bjellqvist 9.823
Wikipedia 10.306
Rodwell 10.54
Grimsley 10.218
Solomon 10.204
Lehninger 10.175
Nozaki 9.999
DTASelect 9.809
Thurlkill 10.028
EMBOSS 10.379
Sillero 10.072
Patrickios 10.189
IPC_peptide 10.204
IPC2_peptide 8.419
IPC2.peptide.svr19 8.085
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1406
256
813
468.7
51.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.597 ± 1.474
2.418 ± 0.679
6.046 ± 0.841
5.477 ± 0.937
3.912 ± 0.622
8.748 ± 0.749
3.129 ± 0.735
4.339 ± 1.042
4.054 ± 0.991
7.397 ± 1.388
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.489 ± 0.373
2.418 ± 0.266
5.05 ± 0.734
3.841 ± 0.422
7.895 ± 0.768
5.974 ± 1.484
5.334 ± 0.621
6.543 ± 0.52
1.991 ± 0.357
2.347 ± 0.3
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here