Burkholderiales bacterium PBB4

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; unclassified Burkholderiales

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5338 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A257GXC5|A0A257GXC5_9BURK GGDEF domain-containing protein (Fragment) OS=Burkholderiales bacterium PBB4 OX=2015566 GN=CFE44_20605 PE=4 SV=1
MM1 pKa = 7.47NSIKK5 pKa = 10.55LALAGALLAGSGASMADD22 pKa = 3.92PIVVDD27 pKa = 3.86AGWYY31 pKa = 8.29GFCFDD36 pKa = 5.23AGAGMPAFAGGCKK49 pKa = 10.07NSGVGEE55 pKa = 4.22AGNPFTFSLSGNGVLKK71 pKa = 10.15VTDD74 pKa = 3.6AFQYY78 pKa = 11.47GDD80 pKa = 3.48IFDD83 pKa = 4.1VFINGALVPTFTTSAPGNGFEE104 pKa = 5.23TPDD107 pKa = 3.76PDD109 pKa = 3.7AAFDD113 pKa = 3.77GGLYY117 pKa = 10.58SSGSLLLGAGNYY129 pKa = 10.43SVMIYY134 pKa = 9.98TKK136 pKa = 10.18ATVV139 pKa = 2.79

Molecular weight: 13.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A257GWI0|A0A257GWI0_9BURK Class I poly(R)-hydroxyalkanoic acid synthase (Fragment) OS=Burkholderiales bacterium PBB4 OX=2015566 GN=CFE44_22075 PE=4 SV=1
MM1 pKa = 7.91PKK3 pKa = 9.67MKK5 pKa = 9.88TKK7 pKa = 10.49SAAKK11 pKa = 9.4KK12 pKa = 9.52RR13 pKa = 11.84FRR15 pKa = 11.84VRR17 pKa = 11.84PGGTVKK23 pKa = 10.62RR24 pKa = 11.84GQAFKK29 pKa = 11.01RR30 pKa = 11.84HH31 pKa = 5.79ILTKK35 pKa = 9.78KK36 pKa = 3.94TTKK39 pKa = 10.22NKK41 pKa = 8.75RR42 pKa = 11.84HH43 pKa = 5.84LRR45 pKa = 11.84GAVTVHH51 pKa = 5.19STNMGHH57 pKa = 6.4MAQMLPGQGII67 pKa = 3.67

Molecular weight: 7.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5338

0

5338

1380797

23

1906

258.7

28.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.134 ± 0.044

0.964 ± 0.014

5.13 ± 0.025

5.029 ± 0.031

3.635 ± 0.022

8.001 ± 0.034

2.258 ± 0.018

4.405 ± 0.026

3.863 ± 0.029

10.693 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.529 ± 0.02

2.928 ± 0.022

4.999 ± 0.024

4.289 ± 0.023

6.054 ± 0.027

6.012 ± 0.03

5.559 ± 0.03

7.69 ± 0.033

1.538 ± 0.017

2.285 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski