Escherichia phage ECD7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Krischvirus; Escherichia virus ECD7

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 262 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A220NTR6|A0A220NTR6_9CAUD Polynucleotide 5'-kinase and 3'-phosphatase OS=Escherichia phage ECD7 OX=1981499 GN=pseT_2 PE=4 SV=1
MM1 pKa = 7.54TKK3 pKa = 10.06LKK5 pKa = 10.43CVAVDD10 pKa = 4.67FEE12 pKa = 4.61LADD15 pKa = 3.71SFVVGDD21 pKa = 4.37EE22 pKa = 3.65YY23 pKa = 11.43DD24 pKa = 3.34ARR26 pKa = 11.84HH27 pKa = 6.96DD28 pKa = 3.83GTCYY32 pKa = 9.94YY33 pKa = 11.02VKK35 pKa = 10.57DD36 pKa = 3.86EE37 pKa = 4.9YY38 pKa = 11.22GVEE41 pKa = 4.01WAILPDD47 pKa = 4.02DD48 pKa = 3.7KK49 pKa = 11.27CFEE52 pKa = 4.44VVGG55 pKa = 4.07

Molecular weight: 6.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A220NT43|A0A220NT43_9CAUD Uncharacterized protein OS=Escherichia phage ECD7 OX=1981499 GN=ECD7_00003 PE=4 SV=1
MM1 pKa = 7.45TKK3 pKa = 9.22LTAIQKK9 pKa = 6.9EE10 pKa = 4.63TIRR13 pKa = 11.84KK14 pKa = 8.18QYY16 pKa = 11.3ARR18 pKa = 11.84GLTKK22 pKa = 10.94GEE24 pKa = 3.84LARR27 pKa = 11.84IYY29 pKa = 10.42NVSDD33 pKa = 2.97RR34 pKa = 11.84TIRR37 pKa = 11.84KK38 pKa = 9.0VVEE41 pKa = 4.23AKK43 pKa = 10.51

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

262

0

262

50230

36

1246

191.7

21.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.719 ± 0.19

1.3 ± 0.079

6.643 ± 0.105

7.496 ± 0.199

4.414 ± 0.099

6.213 ± 0.201

1.999 ± 0.075

6.944 ± 0.141

7.677 ± 0.185

7.249 ± 0.135

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.03 ± 0.095

5.268 ± 0.124

3.253 ± 0.1

3.062 ± 0.088

4.844 ± 0.097

5.558 ± 0.127

5.815 ± 0.179

6.906 ± 0.136

1.366 ± 0.057

4.244 ± 0.113
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski