Gordonia phage Gustav
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H4PA60|A0A2H4PA60_9CAUD Uncharacterized protein OS=Gordonia phage Gustav OX=2047872 GN=PHIRE_GUSTAV_56 PE=4 SV=1
MM1 pKa = 7.81 SDD3 pKa = 3.11 TVVTIHH9 pKa = 5.86 YY10 pKa = 8.06 QSPADD15 pKa = 4.43 DD16 pKa = 4.18 PFKK19 pKa = 10.97 VPRR22 pKa = 11.84 PTRR25 pKa = 11.84 IQFDD29 pKa = 3.35 AAGNIQTTGGEE40 pKa = 4.38 GEE42 pKa = 4.06 ILGDD46 pKa = 3.58 QLIGLSRR53 pKa = 11.84 QDD55 pKa = 3.8 GPPEE59 pKa = 4.26 SFDD62 pKa = 3.37 AEE64 pKa = 3.99 NWVEE68 pKa = 4.0 AEE70 pKa = 4.48 EE71 pKa = 4.11 ILSEE75 pKa = 4.25 GVADD79 pKa = 5.19 LEE81 pKa = 4.38 LAGWFPAFMSGEE93 pKa = 4.41 GIYY96 pKa = 8.72 TWSGAVDD103 pKa = 3.4 RR104 pKa = 11.84 VEE106 pKa = 4.18 VTVQQ110 pKa = 2.76
Molecular weight: 11.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.251
IPC2_protein 3.846
IPC_protein 3.783
Toseland 3.592
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.617
Grimsley 3.503
Solomon 3.732
Lehninger 3.694
Nozaki 3.884
DTASelect 4.037
Thurlkill 3.643
EMBOSS 3.681
Sillero 3.897
Patrickios 3.567
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.827
Protein with the highest isoelectric point:
>tr|A0A2H4PA26|A0A2H4PA26_9CAUD Uncharacterized protein OS=Gordonia phage Gustav OX=2047872 GN=PHIRE_GUSTAV_28 PE=4 SV=1
MM1 pKa = 7.35 EE2 pKa = 6.22 ADD4 pKa = 3.78 LSAYY8 pKa = 9.67 HH9 pKa = 6.82 HH10 pKa = 7.26 LDD12 pKa = 3.08 LRR14 pKa = 11.84 DD15 pKa = 3.02 RR16 pKa = 11.84 WRR18 pKa = 11.84 YY19 pKa = 10.13 EE20 pKa = 3.86 EE21 pKa = 4.24 VLLASGHH28 pKa = 5.24 RR29 pKa = 11.84 VVLRR33 pKa = 11.84 KK34 pKa = 8.68 LTTRR38 pKa = 11.84 MIYY41 pKa = 10.02 VRR43 pKa = 11.84 IRR45 pKa = 11.84 HH46 pKa = 5.61 GLPPEE51 pKa = 4.15 SALAIHH57 pKa = 6.59 FNGGKK62 pKa = 9.02 WPWKK66 pKa = 10.61 LGDD69 pKa = 4.31 HH70 pKa = 6.89 LLADD74 pKa = 4.93 LYY76 pKa = 10.91 FLHH79 pKa = 6.7 RR80 pKa = 11.84 QEE82 pKa = 5.91 LEE84 pKa = 3.81 GKK86 pKa = 9.0 KK87 pKa = 10.68 AKK89 pKa = 10.09 DD90 pKa = 3.14 HH91 pKa = 6.87 PGRR94 pKa = 11.84 PRR96 pKa = 11.84 PDD98 pKa = 3.0 KK99 pKa = 10.7 TSTVSAARR107 pKa = 11.84 ARR109 pKa = 11.84 KK110 pKa = 8.46 MRR112 pKa = 11.84 DD113 pKa = 2.75 ARR115 pKa = 11.84 RR116 pKa = 11.84 RR117 pKa = 11.84 AAAEE121 pKa = 3.64 EE122 pKa = 3.53 ARR124 pKa = 11.84 RR125 pKa = 11.84 NYY127 pKa = 9.92 RR128 pKa = 11.84 RR129 pKa = 11.84 SQGGGG134 pKa = 3.02
Molecular weight: 15.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.794
IPC_protein 10.804
Toseland 10.877
ProMoST 10.745
Dawson 10.95
Bjellqvist 10.73
Wikipedia 11.228
Rodwell 11.038
Grimsley 10.994
Solomon 11.155
Lehninger 11.096
Nozaki 10.847
DTASelect 10.73
Thurlkill 10.877
EMBOSS 11.301
Sillero 10.891
Patrickios 10.76
IPC_peptide 11.155
IPC2_peptide 9.706
IPC2.peptide.svr19 8.607
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
69
0
69
14241
33
1495
206.4
22.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.211 ± 0.478
0.59 ± 0.092
6.509 ± 0.274
6.039 ± 0.334
2.472 ± 0.146
9.016 ± 0.443
1.952 ± 0.151
4.108 ± 0.145
3.68 ± 0.211
8.153 ± 0.231
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.191 ± 0.149
2.97 ± 0.234
6.046 ± 0.313
3.265 ± 0.16
6.727 ± 0.383
5.091 ± 0.21
7.078 ± 0.277
7.436 ± 0.212
2.086 ± 0.126
2.38 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here