Streptococcus phage SW21

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus; unclassified Moineauvirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 39 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S5H0V9|A0A3S5H0V9_9CAUD Head-tail connector protein OS=Streptococcus phage SW21 OX=2419642 GN=SW21_009 PE=4 SV=1
MM1 pKa = 7.3SVSKK5 pKa = 9.08EE6 pKa = 4.26TIMQTLNLDD15 pKa = 3.8EE16 pKa = 4.97TDD18 pKa = 3.78DD19 pKa = 3.8TALIPAYY26 pKa = 9.65IEE28 pKa = 3.9SARR31 pKa = 11.84QYY33 pKa = 10.3VVNSVGDD40 pKa = 3.54DD41 pKa = 3.37PKK43 pKa = 10.95FYY45 pKa = 11.07NLDD48 pKa = 3.41SVRR51 pKa = 11.84ALFDD55 pKa = 3.34TAVIALTSSYY65 pKa = 8.66FTYY68 pKa = 10.07RR69 pKa = 11.84VALTDD74 pKa = 3.37TATYY78 pKa = 9.41PVNLTLNSIIGQLRR92 pKa = 11.84GLYY95 pKa = 8.66ATYY98 pKa = 10.56SEE100 pKa = 4.46EE101 pKa = 5.06RR102 pKa = 11.84GDD104 pKa = 3.53

Molecular weight: 11.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S7W8V9|A0A3S7W8V9_9CAUD Terminase large subunit OS=Streptococcus phage SW21 OX=2419642 GN=SW21_003 PE=4 SV=1
MM1 pKa = 7.56PMVRR5 pKa = 11.84RR6 pKa = 11.84CKK8 pKa = 10.43ADD10 pKa = 2.91SCRR13 pKa = 11.84TLVEE17 pKa = 4.73RR18 pKa = 11.84PAHH21 pKa = 5.79YY22 pKa = 8.38CTAHH26 pKa = 7.09KK27 pKa = 10.81DD28 pKa = 3.29MEE30 pKa = 4.31AQEE33 pKa = 4.1RR34 pKa = 11.84QRR36 pKa = 11.84YY37 pKa = 8.7SRR39 pKa = 11.84TRR41 pKa = 11.84YY42 pKa = 5.83NTRR45 pKa = 11.84VRR47 pKa = 11.84NRR49 pKa = 11.84DD50 pKa = 3.33DD51 pKa = 3.47EE52 pKa = 4.57TKK54 pKa = 10.41EE55 pKa = 3.75RR56 pKa = 11.84YY57 pKa = 9.08AFYY60 pKa = 10.6RR61 pKa = 11.84SRR63 pKa = 11.84VWSSIRR69 pKa = 11.84KK70 pKa = 8.48VALEE74 pKa = 4.0RR75 pKa = 11.84DD76 pKa = 3.58NYY78 pKa = 10.08LCQYY82 pKa = 10.11CLALGVTTPDD92 pKa = 3.19ARR94 pKa = 11.84IGDD97 pKa = 3.91HH98 pKa = 5.15VTPVEE103 pKa = 3.97IAPEE107 pKa = 4.07LKK109 pKa = 9.69TDD111 pKa = 3.36VSNIVATCRR120 pKa = 11.84SCDD123 pKa = 3.27NTKK126 pKa = 9.28RR127 pKa = 11.84TLEE130 pKa = 3.83QEE132 pKa = 4.04IYY134 pKa = 9.73GTGQNRR140 pKa = 11.84TKK142 pKa = 10.92QNTDD146 pKa = 2.61LRR148 pKa = 11.84LSVAAWSGLIARR160 pKa = 11.84KK161 pKa = 9.55KK162 pKa = 10.19ADD164 pKa = 3.35VVKK167 pKa = 10.34PLL169 pKa = 3.7

Molecular weight: 19.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

39

0

39

10604

59

1591

271.9

30.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.46 ± 0.492

0.481 ± 0.136

6.648 ± 0.232

6.347 ± 0.487

4.121 ± 0.22

6.743 ± 0.522

1.405 ± 0.133

6.686 ± 0.346

8.252 ± 0.455

7.931 ± 0.399

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.424 ± 0.195

6.375 ± 0.323

3.121 ± 0.197

4.121 ± 0.243

4.055 ± 0.318

6.535 ± 0.304

6.526 ± 0.282

6.224 ± 0.212

1.594 ± 0.174

3.951 ± 0.27
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski