Aeromonas phage Aswh_1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Emmerichvirinae; Ishigurovirus; unclassified Ishigurovirus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 406 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411BHE2|A0A411BHE2_9CAUD Uncharacterized protein OS=Aeromonas phage Aswh_1 OX=2419740 GN=ASwh1_39 PE=4 SV=1
MM1 pKa = 7.18NLKK4 pKa = 9.69IKK6 pKa = 10.58CDD8 pKa = 3.57ALVSLWQQSQYY19 pKa = 10.66CDD21 pKa = 2.73NGVFVEE27 pKa = 4.66LEE29 pKa = 4.06GVDD32 pKa = 4.2LSEE35 pKa = 5.74LIQEE39 pKa = 4.2LVINNSLSDD48 pKa = 3.38IVHH51 pKa = 6.79EE52 pKa = 4.66IGLEE56 pKa = 3.9KK57 pKa = 10.77LQDD60 pKa = 4.89CIDD63 pKa = 3.67EE64 pKa = 4.74TYY66 pKa = 11.5SNLDD70 pKa = 3.66GII72 pKa = 4.78

Molecular weight: 8.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411BIA9|A0A411BIA9_9CAUD Uncharacterized protein OS=Aeromonas phage Aswh_1 OX=2419740 GN=ASwh1_283 PE=4 SV=1
MM1 pKa = 7.49SGLNRR6 pKa = 11.84SPAKK10 pKa = 9.94GVNRR14 pKa = 11.84KK15 pKa = 7.54VSRR18 pKa = 11.84VRR20 pKa = 11.84ISLSPPNIEE29 pKa = 4.91DD30 pKa = 3.29VVEE33 pKa = 4.19LVNIPACHH41 pKa = 5.65VGDD44 pKa = 3.7RR45 pKa = 11.84GFEE48 pKa = 3.98PRR50 pKa = 11.84HH51 pKa = 5.43PRR53 pKa = 11.84QIRR56 pKa = 11.84PISAFNEE63 pKa = 4.21TT64 pKa = 3.9

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

406

0

406

69972

37

1533

172.3

19.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.923 ± 0.124

1.331 ± 0.059

6.704 ± 0.108

7.114 ± 0.186

4.716 ± 0.099

6.407 ± 0.194

1.978 ± 0.07

7.103 ± 0.097

7.706 ± 0.206

7.059 ± 0.098

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.126 ± 0.091

5.578 ± 0.12

3.226 ± 0.096

3.136 ± 0.076

4.223 ± 0.079

6.691 ± 0.122

5.597 ± 0.169

7.276 ± 0.115

1.402 ± 0.053

4.706 ± 0.121
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski