Rio Grande virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Rio Grande phlebovirus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C0E4I4|A0A5C0E4I4_9VIRU Polyprotein G1G2 OS=Rio Grande virus OX=629740 PE=4 SV=1
MM1 pKa = 7.38EE2 pKa = 5.46NYY4 pKa = 9.78YY5 pKa = 9.93IHH7 pKa = 7.73DD8 pKa = 3.76MPFVRR13 pKa = 11.84RR14 pKa = 11.84TGGRR18 pKa = 11.84ASVHH22 pKa = 5.08FRR24 pKa = 11.84ASLSYY29 pKa = 10.65IEE31 pKa = 4.58WPVANYY37 pKa = 10.89YY38 pKa = 11.16GMEE41 pKa = 4.19FPIKK45 pKa = 9.86HH46 pKa = 5.28YY47 pKa = 10.73QVDD50 pKa = 4.13LVSRR54 pKa = 11.84ARR56 pKa = 11.84FNEE59 pKa = 3.79FFDD62 pKa = 4.98RR63 pKa = 11.84GFLPLRR69 pKa = 11.84ISSEE73 pKa = 3.89ADD75 pKa = 3.13SQVPVSPPEE84 pKa = 3.75PEE86 pKa = 5.02GIFEE90 pKa = 4.62FLACQTEE97 pKa = 4.38EE98 pKa = 4.59SISSANEE105 pKa = 3.54EE106 pKa = 4.27YY107 pKa = 10.12MLEE110 pKa = 4.52AISWPLGRR118 pKa = 11.84PSLGFFRR125 pKa = 11.84HH126 pKa = 5.58YY127 pKa = 10.59YY128 pKa = 9.57NRR130 pKa = 11.84GTRR133 pKa = 11.84RR134 pKa = 11.84SWIQRR139 pKa = 11.84SMLASDD145 pKa = 4.98LLNASGYY152 pKa = 11.0DD153 pKa = 3.82CLCCAFPKK161 pKa = 10.46VYY163 pKa = 11.04SNVLSIAQRR172 pKa = 11.84MDD174 pKa = 3.41LDD176 pKa = 4.67LNLFTGNDD184 pKa = 3.14IVKK187 pKa = 8.78EE188 pKa = 3.98ICHH191 pKa = 5.24IQCVKK196 pKa = 9.56MMKK199 pKa = 9.82AALMEE204 pKa = 4.32RR205 pKa = 11.84SMGTQLSPSTEE216 pKa = 4.66LILTAMGEE224 pKa = 4.34ATGDD228 pKa = 3.21RR229 pKa = 11.84DD230 pKa = 4.28PIMEE234 pKa = 4.44EE235 pKa = 3.98LLKK238 pKa = 9.9EE239 pKa = 4.17TAALPSPPPTPP250 pKa = 3.62

Molecular weight: 28.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8D7T4|A0A4P8D7T4_9VIRU Replicase OS=Rio Grande virus OX=629740 PE=4 SV=1
MM1 pKa = 7.48SFAKK5 pKa = 10.06LAKK8 pKa = 10.28DD9 pKa = 3.53LGLEE13 pKa = 4.03VLDD16 pKa = 5.06LKK18 pKa = 10.91EE19 pKa = 3.72IQQWVSEE26 pKa = 4.04FAYY29 pKa = 10.52QGFDD33 pKa = 3.06ANRR36 pKa = 11.84VVEE39 pKa = 4.32LVQEE43 pKa = 4.18RR44 pKa = 11.84AKK46 pKa = 10.42GRR48 pKa = 11.84KK49 pKa = 5.03WQEE52 pKa = 3.49DD53 pKa = 4.02VKK55 pKa = 11.35RR56 pKa = 11.84MIILALTRR64 pKa = 11.84GNKK67 pKa = 7.63PDD69 pKa = 3.45KK70 pKa = 9.5MRR72 pKa = 11.84KK73 pKa = 9.1KK74 pKa = 9.84MSPEE78 pKa = 4.44GIAVLDD84 pKa = 4.45DD85 pKa = 3.86LVKK88 pKa = 9.89TYY90 pKa = 8.2QLKK93 pKa = 10.47SSSPGRR99 pKa = 11.84DD100 pKa = 3.39DD101 pKa = 3.58LTLARR106 pKa = 11.84IAAAFAPWTCQATEE120 pKa = 3.76AVEE123 pKa = 4.76NYY125 pKa = 9.75MPVNGAAMDD134 pKa = 4.21EE135 pKa = 4.23LSKK138 pKa = 11.08NYY140 pKa = 8.08PRR142 pKa = 11.84PMMHH146 pKa = 7.23PAFAGLIDD154 pKa = 4.86PSLKK158 pKa = 10.08PQQLDD163 pKa = 3.33IVVKK167 pKa = 10.25AHH169 pKa = 6.67SLFLLRR175 pKa = 11.84FSKK178 pKa = 10.8VINVNLRR185 pKa = 11.84GKK187 pKa = 9.19PKK189 pKa = 10.31HH190 pKa = 6.52DD191 pKa = 3.66VEE193 pKa = 5.69LSFKK197 pKa = 10.77QPLTAAVNSNFLTGEE212 pKa = 3.85EE213 pKa = 4.01RR214 pKa = 11.84RR215 pKa = 11.84EE216 pKa = 3.58ILIKK220 pKa = 10.82LKK222 pKa = 10.38IVDD225 pKa = 4.13RR226 pKa = 11.84NGEE229 pKa = 3.73ATTNVVRR236 pKa = 11.84AAEE239 pKa = 4.46AYY241 pKa = 10.2DD242 pKa = 3.8LEE244 pKa = 4.67SS245 pKa = 3.4

Molecular weight: 27.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3955

245

2094

988.8

111.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.992 ± 1.083

2.554 ± 0.84

5.613 ± 0.758

7.13 ± 0.208

4.779 ± 0.284

5.992 ± 0.585

2.402 ± 0.303

6.422 ± 0.539

6.549 ± 0.938

8.647 ± 0.666

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.009 ± 0.518

4.121 ± 0.214

3.767 ± 0.973

3.413 ± 0.237

5.461 ± 0.527

9.178 ± 0.793

4.753 ± 0.349

6.094 ± 0.628

1.239 ± 0.036

2.882 ± 0.421
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski