Amino acid dipepetide frequency for Actinotignum schaalii FB123-CNA-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.745AlaAla: 18.745 ± 0.3	0.994AlaCys: 0.994 ± 0.041	6.656AlaAsp: 6.656 ± 0.105	7.621AlaGlu: 7.621 ± 0.145	3.368AlaPhe: 3.368 ± 0.091	12.291AlaGly: 12.291 ± 0.177	2.635AlaHis: 2.635 ± 0.07	5.205AlaIle: 5.205 ± 0.104	3.211AlaLys: 3.211 ± 0.108	12.996AlaLeu: 12.996 ± 0.221	2.62AlaMet: 2.62 ± 0.075	2.766AlaAsn: 2.766 ± 0.073	6.681AlaPro: 6.681 ± 0.159	4.872AlaGln: 4.872 ± 0.109	9.861AlaArg: 9.861 ± 0.19	7.043AlaSer: 7.043 ± 0.127	7.344AlaThr: 7.344 ± 0.143	9.77AlaVal: 9.77 ± 0.17	1.641AlaTrp: 1.641 ± 0.054	2.503AlaTyr: 2.503 ± 0.06	0.0AlaXaa: 0.0 ± 0.0
Cys	1.009CysAla: 1.009 ± 0.045	0.073CysCys: 0.073 ± 0.013	0.374CysAsp: 0.374 ± 0.024	0.447CysGlu: 0.447 ± 0.031	0.263CysPhe: 0.263 ± 0.02	0.88CysGly: 0.88 ± 0.035	0.165CysHis: 0.165 ± 0.019	0.294CysIle: 0.294 ± 0.023	0.104CysLys: 0.104 ± 0.012	0.679CysLeu: 0.679 ± 0.034	0.129CysMet: 0.129 ± 0.014	0.168CysAsn: 0.168 ± 0.017	0.42CysPro: 0.42 ± 0.029	0.222CysGln: 0.222 ± 0.019	0.397CysArg: 0.397 ± 0.027	0.475CysSer: 0.475 ± 0.032	0.473CysThr: 0.473 ± 0.027	0.616CysVal: 0.616 ± 0.038	0.08CysTrp: 0.08 ± 0.012	0.176CysTyr: 0.176 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	6.867AspAla: 6.867 ± 0.118	0.34AspCys: 0.34 ± 0.024	2.976AspAsp: 2.976 ± 0.084	3.334AspGlu: 3.334 ± 0.08	1.931AspPhe: 1.931 ± 0.059	4.625AspGly: 4.625 ± 0.149	1.06AspHis: 1.06 ± 0.043	3.435AspIle: 3.435 ± 0.08	1.507AspLys: 1.507 ± 0.071	5.087AspLeu: 5.087 ± 0.101	1.197AspMet: 1.197 ± 0.046	1.422AspAsn: 1.422 ± 0.058	3.605AspPro: 3.605 ± 0.087	1.249AspGln: 1.249 ± 0.048	3.06AspArg: 3.06 ± 0.085	3.048AspSer: 3.048 ± 0.075	3.525AspThr: 3.525 ± 0.093	4.483AspVal: 4.483 ± 0.091	0.782AspTrp: 0.782 ± 0.049	1.422AspTyr: 1.422 ± 0.058	0.0AspXaa: 0.0 ± 0.0
Glu	7.31GluAla: 7.31 ± 0.135	0.335GluCys: 0.335 ± 0.026	3.229GluAsp: 3.229 ± 0.085	4.735GluGlu: 4.735 ± 0.117	1.827GluPhe: 1.827 ± 0.055	4.349GluGly: 4.349 ± 0.099	1.447GluHis: 1.447 ± 0.054	3.198GluIle: 3.198 ± 0.081	2.568GluLys: 2.568 ± 0.089	6.511GluLeu: 6.511 ± 0.118	1.27GluMet: 1.27 ± 0.05	2.08GluAsn: 2.08 ± 0.066	2.939GluPro: 2.939 ± 0.096	2.082GluGln: 2.082 ± 0.069	4.857GluArg: 4.857 ± 0.114	3.257GluSer: 3.257 ± 0.084	3.344GluThr: 3.344 ± 0.087	4.509GluVal: 4.509 ± 0.094	0.795GluTrp: 0.795 ± 0.034	1.402GluTyr: 1.402 ± 0.043	0.0GluXaa: 0.0 ± 0.0
Phe	3.847PheAla: 3.847 ± 0.097	0.253PheCys: 0.253 ± 0.019	1.941PheAsp: 1.941 ± 0.053	1.664PheGlu: 1.664 ± 0.057	1.269PhePhe: 1.269 ± 0.055	3.145PheGly: 3.145 ± 0.094	0.611PheHis: 0.611 ± 0.033	1.496PheIle: 1.496 ± 0.054	0.648PheLys: 0.648 ± 0.04	2.859PheLeu: 2.859 ± 0.072	0.529PheMet: 0.529 ± 0.031	0.825PheAsn: 0.825 ± 0.042	1.58PhePro: 1.58 ± 0.053	0.854PheGln: 0.854 ± 0.033	1.625PheArg: 1.625 ± 0.061	2.136PheSer: 2.136 ± 0.062	2.251PheThr: 2.251 ± 0.075	2.389PheVal: 2.389 ± 0.061	0.464PheTrp: 0.464 ± 0.03	0.779PheTyr: 0.779 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	10.803GlyAla: 10.803 ± 0.175	0.73GlyCys: 0.73 ± 0.034	4.418GlyAsp: 4.418 ± 0.125	5.452GlyGlu: 5.452 ± 0.094	2.834GlyPhe: 2.834 ± 0.074	7.247GlyGly: 7.247 ± 0.164	1.754GlyHis: 1.754 ± 0.059	4.795GlyIle: 4.795 ± 0.1	3.203GlyLys: 3.203 ± 0.078	7.481GlyLeu: 7.481 ± 0.136	2.158GlyMet: 2.158 ± 0.064	2.514GlyAsn: 2.514 ± 0.088	3.442GlyPro: 3.442 ± 0.089	2.813GlyGln: 2.813 ± 0.067	5.61GlyArg: 5.61 ± 0.1	5.12GlySer: 5.12 ± 0.094	5.876GlyThr: 5.876 ± 0.114	6.87GlyVal: 6.87 ± 0.122	1.313GlyTrp: 1.313 ± 0.052	2.431GlyTyr: 2.431 ± 0.073	0.0GlyXaa: 0.0 ± 0.0
His	2.361HisAla: 2.361 ± 0.066	0.171HisCys: 0.171 ± 0.016	1.11HisAsp: 1.11 ± 0.049	1.102HisGlu: 1.102 ± 0.041	0.617HisPhe: 0.617 ± 0.037	1.794HisGly: 1.794 ± 0.065	0.537HisHis: 0.537 ± 0.036	1.053HisIle: 1.053 ± 0.044	0.385HisLys: 0.385 ± 0.022	1.961HisLeu: 1.961 ± 0.069	0.393HisMet: 0.393 ± 0.025	0.55HisAsn: 0.55 ± 0.029	1.509HisPro: 1.509 ± 0.048	0.532HisGln: 0.532 ± 0.032	1.53HisArg: 1.53 ± 0.057	1.133HisSer: 1.133 ± 0.052	1.265HisThr: 1.265 ± 0.048	1.538HisVal: 1.538 ± 0.05	0.276HisTrp: 0.276 ± 0.025	0.534HisTyr: 0.534 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	6.413IleAla: 6.413 ± 0.112	0.439IleCys: 0.439 ± 0.033	2.986IleAsp: 2.986 ± 0.079	2.568IleGlu: 2.568 ± 0.081	1.861IlePhe: 1.861 ± 0.061	4.237IleGly: 4.237 ± 0.097	0.924IleHis: 0.924 ± 0.041	2.733IleIle: 2.733 ± 0.076	1.174IleLys: 1.174 ± 0.061	4.299IleLeu: 4.299 ± 0.088	0.922IleMet: 0.922 ± 0.037	1.36IleAsn: 1.36 ± 0.051	2.993IlePro: 2.993 ± 0.073	1.138IleGln: 1.138 ± 0.046	2.815IleArg: 2.815 ± 0.077	3.083IleSer: 3.083 ± 0.078	3.383IleThr: 3.383 ± 0.084	3.845IleVal: 3.845 ± 0.091	0.545IleTrp: 0.545 ± 0.033	1.131IleTyr: 1.131 ± 0.045	0.0IleXaa: 0.0 ± 0.0
Lys	2.932LysAla: 2.932 ± 0.094	0.126LysCys: 0.126 ± 0.014	1.598LysAsp: 1.598 ± 0.069	1.789LysGlu: 1.789 ± 0.054	0.751LysPhe: 0.751 ± 0.037	1.84LysGly: 1.84 ± 0.059	0.531LysHis: 0.531 ± 0.027	1.644LysIle: 1.644 ± 0.068	1.461LysLys: 1.461 ± 0.07	2.552LysLeu: 2.552 ± 0.075	0.787LysMet: 0.787 ± 0.035	1.182LysAsn: 1.182 ± 0.058	1.505LysPro: 1.505 ± 0.065	0.829LysGln: 0.829 ± 0.04	1.773LysArg: 1.773 ± 0.058	1.393LysSer: 1.393 ± 0.051	1.598LysThr: 1.598 ± 0.053	2.202LysVal: 2.202 ± 0.07	0.271LysTrp: 0.271 ± 0.02	0.696LysTyr: 0.696 ± 0.035	0.0LysXaa: 0.0 ± 0.0
Leu	13.558LeuAla: 13.558 ± 0.228	0.758LeuCys: 0.758 ± 0.036	5.341LeuAsp: 5.341 ± 0.108	5.595LeuGlu: 5.595 ± 0.103	2.647LeuPhe: 2.647 ± 0.089	8.487LeuGly: 8.487 ± 0.146	1.74LeuHis: 1.74 ± 0.054	4.46LeuIle: 4.46 ± 0.105	2.067LeuLys: 2.067 ± 0.06	9.295LeuLeu: 9.295 ± 0.174	1.739LeuMet: 1.739 ± 0.052	2.263LeuAsn: 2.263 ± 0.065	5.37LeuPro: 5.37 ± 0.101	2.17LeuGln: 2.17 ± 0.061	7.071LeuArg: 7.071 ± 0.109	6.139LeuSer: 6.139 ± 0.126	6.336LeuThr: 6.336 ± 0.11	7.261LeuVal: 7.261 ± 0.127	1.113LeuTrp: 1.113 ± 0.051	1.848LeuTyr: 1.848 ± 0.058	0.0LeuXaa: 0.0 ± 0.0
Met	2.366MetAla: 2.366 ± 0.064	0.173MetCys: 0.173 ± 0.016	1.159MetAsp: 1.159 ± 0.044	1.097MetGlu: 1.097 ± 0.042	0.651MetPhe: 0.651 ± 0.033	1.602MetGly: 1.602 ± 0.06	0.376MetHis: 0.376 ± 0.028	0.978MetIle: 0.978 ± 0.045	0.673MetLys: 0.673 ± 0.033	1.809MetLeu: 1.809 ± 0.056	0.438MetMet: 0.438 ± 0.029	0.62MetAsn: 0.62 ± 0.035	1.045MetPro: 1.045 ± 0.038	0.542MetGln: 0.542 ± 0.029	1.582MetArg: 1.582 ± 0.052	1.523MetSer: 1.523 ± 0.052	1.504MetThr: 1.504 ± 0.056	1.497MetVal: 1.497 ± 0.047	0.248MetTrp: 0.248 ± 0.018	0.408MetTyr: 0.408 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	3.03AsnAla: 3.03 ± 0.086	0.162AsnCys: 0.162 ± 0.016	1.412AsnAsp: 1.412 ± 0.056	1.378AsnGlu: 1.378 ± 0.056	0.911AsnPhe: 0.911 ± 0.044	2.315AsnGly: 2.315 ± 0.089	0.478AsnHis: 0.478 ± 0.038	1.456AsnIle: 1.456 ± 0.052	0.754AsnLys: 0.754 ± 0.035	2.49AsnLeu: 2.49 ± 0.066	0.609AsnMet: 0.609 ± 0.035	0.882AsnAsn: 0.882 ± 0.045	2.096AsnPro: 2.096 ± 0.069	0.771AsnGln: 0.771 ± 0.036	1.523AsnArg: 1.523 ± 0.044	1.589AsnSer: 1.589 ± 0.059	1.579AsnThr: 1.579 ± 0.062	1.977AsnVal: 1.977 ± 0.074	0.377AsnTrp: 0.377 ± 0.029	0.779AsnTyr: 0.779 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	7.891ProAla: 7.891 ± 0.178	0.291ProCys: 0.291 ± 0.023	3.38ProAsp: 3.38 ± 0.086	4.573ProGlu: 4.573 ± 0.112	1.509ProPhe: 1.509 ± 0.05	5.383ProGly: 5.383 ± 0.138	1.176ProHis: 1.176 ± 0.042	2.011ProIle: 2.011 ± 0.057	1.184ProLys: 1.184 ± 0.048	4.565ProLeu: 4.565 ± 0.094	0.795ProMet: 0.795 ± 0.041	1.401ProAsn: 1.401 ± 0.06	2.078ProPro: 2.078 ± 0.106	1.811ProGln: 1.811 ± 0.081	3.239ProArg: 3.239 ± 0.086	3.051ProSer: 3.051 ± 0.096	3.345ProThr: 3.345 ± 0.123	4.421ProVal: 4.421 ± 0.09	0.733ProTrp: 0.733 ± 0.037	1.231ProTyr: 1.231 ± 0.05	0.0ProXaa: 0.0 ± 0.0
Gln	3.941GlnAla: 3.941 ± 0.081	0.201GlnCys: 0.201 ± 0.02	1.265GlnAsp: 1.265 ± 0.045	1.687GlnGlu: 1.687 ± 0.057	0.846GlnPhe: 0.846 ± 0.042	1.998GlnGly: 1.998 ± 0.057	0.65GlnHis: 0.65 ± 0.035	1.582GlnIle: 1.582 ± 0.06	0.825GlnLys: 0.825 ± 0.039	3.52GlnLeu: 3.52 ± 0.083	0.671GlnMet: 0.671 ± 0.034	0.81GlnAsn: 0.81 ± 0.042	1.842GlnPro: 1.842 ± 0.092	1.123GlnGln: 1.123 ± 0.053	2.661GlnArg: 2.661 ± 0.081	1.463GlnSer: 1.463 ± 0.044	1.314GlnThr: 1.314 ± 0.053	2.503GlnVal: 2.503 ± 0.064	0.456GlnTrp: 0.456 ± 0.028	0.64GlnTyr: 0.64 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	9.138ArgAla: 9.138 ± 0.18	0.513ArgCys: 0.513 ± 0.03	3.842ArgAsp: 3.842 ± 0.089	5.216ArgGlu: 5.216 ± 0.113	2.093ArgPhe: 2.093 ± 0.066	5.597ArgGly: 5.597 ± 0.101	1.492ArgHis: 1.492 ± 0.048	3.497ArgIle: 3.497 ± 0.087	1.807ArgLys: 1.807 ± 0.055	6.08ArgLeu: 6.08 ± 0.111	1.644ArgMet: 1.644 ± 0.048	1.673ArgAsn: 1.673 ± 0.059	3.12ArgPro: 3.12 ± 0.074	1.977ArgGln: 1.977 ± 0.059	5.465ArgArg: 5.465 ± 0.123	3.948ArgSer: 3.948 ± 0.094	3.99ArgThr: 3.99 ± 0.092	5.236ArgVal: 5.236 ± 0.099	1.071ArgTrp: 1.071 ± 0.042	1.876ArgTyr: 1.876 ± 0.061	0.0ArgXaa: 0.0 ± 0.0
Ser	7.275SerAla: 7.275 ± 0.133	0.442SerCys: 0.442 ± 0.026	2.983SerAsp: 2.983 ± 0.069	3.422SerGlu: 3.422 ± 0.086	2.06SerPhe: 2.06 ± 0.058	6.247SerGly: 6.247 ± 0.131	1.252SerHis: 1.252 ± 0.044	2.449SerIle: 2.449 ± 0.063	1.38SerLys: 1.38 ± 0.055	5.285SerLeu: 5.285 ± 0.107	1.164SerMet: 1.164 ± 0.045	1.37SerAsn: 1.37 ± 0.049	3.197SerPro: 3.197 ± 0.077	1.945SerGln: 1.945 ± 0.055	3.819SerArg: 3.819 ± 0.084	3.502SerSer: 3.502 ± 0.082	3.603SerThr: 3.603 ± 0.085	4.353SerVal: 4.353 ± 0.082	0.947SerTrp: 0.947 ± 0.043	1.607SerTyr: 1.607 ± 0.059	0.0SerXaa: 0.0 ± 0.0
Thr	7.68ThrAla: 7.68 ± 0.133	0.418ThrCys: 0.418 ± 0.028	3.228ThrAsp: 3.228 ± 0.106	3.419ThrGlu: 3.419 ± 0.076	1.951ThrPhe: 1.951 ± 0.063	5.67ThrGly: 5.67 ± 0.11	1.267ThrHis: 1.267 ± 0.05	2.971ThrIle: 2.971 ± 0.084	1.546ThrLys: 1.546 ± 0.063	5.858ThrLeu: 5.858 ± 0.102	1.084ThrMet: 1.084 ± 0.038	1.538ThrAsn: 1.538 ± 0.054	4.354ThrPro: 4.354 ± 0.135	1.899ThrGln: 1.899 ± 0.054	3.765ThrArg: 3.765 ± 0.09	3.554ThrSer: 3.554 ± 0.084	4.106ThrThr: 4.106 ± 0.098	5.463ThrVal: 5.463 ± 0.12	0.97ThrTrp: 0.97 ± 0.045	1.517ThrTyr: 1.517 ± 0.057	0.0ThrXaa: 0.0 ± 0.0
Val	9.816ValAla: 9.816 ± 0.143	0.712ValCys: 0.712 ± 0.036	4.803ValAsp: 4.803 ± 0.103	4.774ValGlu: 4.774 ± 0.099	2.428ValPhe: 2.428 ± 0.07	6.038ValGly: 6.038 ± 0.12	1.482ValHis: 1.482 ± 0.05	3.931ValIle: 3.931 ± 0.08	2.034ValLys: 2.034 ± 0.07	7.832ValLeu: 7.832 ± 0.151	1.388ValMet: 1.388 ± 0.049	1.997ValAsn: 1.997 ± 0.058	4.345ValPro: 4.345 ± 0.08	1.904ValGln: 1.904 ± 0.058	5.626ValArg: 5.626 ± 0.1	4.859ValSer: 4.859 ± 0.101	5.236ValThr: 5.236 ± 0.106	7.154ValVal: 7.154 ± 0.12	0.94ValTrp: 0.94 ± 0.037	1.6ValTyr: 1.6 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	1.332TrpAla: 1.332 ± 0.053	0.136TrpCys: 0.136 ± 0.014	0.882TrpAsp: 0.882 ± 0.048	0.81TrpGlu: 0.81 ± 0.038	0.462TrpPhe: 0.462 ± 0.032	0.983TrpGly: 0.983 ± 0.044	0.31TrpHis: 0.31 ± 0.024	0.681TrpIle: 0.681 ± 0.036	0.424TrpLys: 0.424 ± 0.026	1.482TrpLeu: 1.482 ± 0.056	0.34TrpMet: 0.34 ± 0.023	0.531TrpAsn: 0.531 ± 0.033	0.593TrpPro: 0.593 ± 0.029	0.509TrpGln: 0.509 ± 0.027	1.076TrpArg: 1.076 ± 0.042	0.71TrpSer: 0.71 ± 0.038	0.748TrpThr: 0.748 ± 0.035	0.996TrpVal: 0.996 ± 0.041	0.31TrpTrp: 0.31 ± 0.022	0.31TrpTyr: 0.31 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.511TyrAla: 2.511 ± 0.067	0.18TyrCys: 0.18 ± 0.019	1.371TyrAsp: 1.371 ± 0.048	1.363TyrGlu: 1.363 ± 0.052	0.882TyrPhe: 0.882 ± 0.04	2.137TyrGly: 2.137 ± 0.065	0.467TyrHis: 0.467 ± 0.028	0.978TyrIle: 0.978 ± 0.043	0.504TyrLys: 0.504 ± 0.031	2.616TyrLeu: 2.616 ± 0.068	0.411TyrMet: 0.411 ± 0.027	0.617TyrAsn: 0.617 ± 0.034	1.355TyrPro: 1.355 ± 0.054	0.756TyrGln: 0.756 ± 0.033	1.922TyrArg: 1.922 ± 0.065	1.275TyrSer: 1.275 ± 0.05	1.437TyrThr: 1.437 ± 0.049	1.816TyrVal: 1.816 ± 0.064	0.292TyrTrp: 0.292 ± 0.021	0.604TyrTyr: 0.604 ± 0.034	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1726 proteins (612490 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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