Acinetobacter phage YMC-13-01-C62
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A068CDE4|A0A068CDE4_9CAUD Uncharacterized protein OS=Acinetobacter phage YMC-13-01-C62 OX=1505225 GN=BPABA14_00370 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.33 NALILAASVALAACSGKK19 pKa = 9.62 PVITGPYY26 pKa = 8.41 EE27 pKa = 4.1 VEE29 pKa = 4.22 SLDD32 pKa = 3.67 MEE34 pKa = 5.01 HH35 pKa = 6.49 NVAAIKK41 pKa = 10.92 SGDD44 pKa = 3.47 LVLEE48 pKa = 4.32 VEE50 pKa = 4.35 FEE52 pKa = 4.21 GRR54 pKa = 11.84 FFKK57 pKa = 10.87 DD58 pKa = 3.13 GNGFQSWNDD67 pKa = 3.42 VEE69 pKa = 4.56 VEE71 pKa = 4.22 SVNDD75 pKa = 3.26 VKK77 pKa = 11.33 VYY79 pKa = 11.08 NEE81 pKa = 4.28 DD82 pKa = 3.88 GEE84 pKa = 4.71 TEE86 pKa = 4.24 NYY88 pKa = 9.74 VLPSEE93 pKa = 4.22 EE94 pKa = 3.74 VSNIVQVIEE103 pKa = 3.93 NEE105 pKa = 4.04 IAEE108 pKa = 4.36 KK109 pKa = 9.83 MM110 pKa = 3.72
Molecular weight: 12.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.276
IPC2_protein 4.126
IPC_protein 4.012
Toseland 3.859
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.113
Wikipedia 3.808
Rodwell 3.846
Grimsley 3.77
Solomon 3.948
Lehninger 3.897
Nozaki 4.075
DTASelect 4.164
Thurlkill 3.872
EMBOSS 3.834
Sillero 4.113
Patrickios 3.528
IPC_peptide 3.948
IPC2_peptide 4.113
IPC2.peptide.svr19 4.038
Protein with the highest isoelectric point:
>tr|A0A068C8I7|A0A068C8I7_9CAUD Uncharacterized protein OS=Acinetobacter phage YMC-13-01-C62 OX=1505225 GN=BPABA14_00430 PE=4 SV=1
MM1 pKa = 7.31 NPIAILIYY9 pKa = 10.03 FVLSFVFVAVLYY21 pKa = 10.59 LYY23 pKa = 9.68 EE24 pKa = 4.28 AYY26 pKa = 9.53 KK27 pKa = 10.26 RR28 pKa = 11.84 KK29 pKa = 10.12 RR30 pKa = 11.84 YY31 pKa = 7.46 VQRR34 pKa = 11.84 KK35 pKa = 8.76 QDD37 pKa = 3.67 DD38 pKa = 3.52 QRR40 pKa = 11.84 KK41 pKa = 8.64 FNPIRR46 pKa = 11.84 EE47 pKa = 4.37 KK48 pKa = 10.67 VWWRR52 pKa = 11.84 KK53 pKa = 7.81
Molecular weight: 6.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.262
IPC2_protein 9.911
IPC_protein 10.335
Toseland 10.35
ProMoST 10.131
Dawson 10.54
Bjellqvist 10.233
Wikipedia 10.73
Rodwell 10.935
Grimsley 10.613
Solomon 10.584
Lehninger 10.54
Nozaki 10.306
DTASelect 10.233
Thurlkill 10.394
EMBOSS 10.745
Sillero 10.452
Patrickios 10.672
IPC_peptide 10.584
IPC2_peptide 8.946
IPC2.peptide.svr19 8.635
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
84
0
84
13776
40
698
164.0
18.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.947 ± 0.41
1.372 ± 0.152
6.366 ± 0.295
6.765 ± 0.33
4.443 ± 0.218
6.693 ± 0.279
1.662 ± 0.141
7.23 ± 0.27
7.818 ± 0.352
7.84 ± 0.253
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.925 ± 0.173
5.873 ± 0.283
3.085 ± 0.226
3.731 ± 0.213
3.913 ± 0.212
6.294 ± 0.26
5.147 ± 0.316
6.424 ± 0.245
1.43 ± 0.106
4.043 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here