Gilliamella apicola SCGC AB-598-B02
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1967 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A080KKX4|A0A080KKX4_9GAMM Uncharacterized protein OS=Gilliamella apicola SCGC AB-598-B02 OX=1445513 GN=GASC598B02_017370 PE=4 SV=1
MM1 pKa = 7.92 KK2 pKa = 10.15 DD3 pKa = 3.34 QIMLAQQDD11 pKa = 3.65 TNAIIEE17 pKa = 4.27 EE18 pKa = 4.18 LLEE21 pKa = 5.15 DD22 pKa = 4.64 GSDD25 pKa = 3.29 PHH27 pKa = 8.25 ALYY30 pKa = 10.15 IIEE33 pKa = 4.36 HH34 pKa = 6.84 HH35 pKa = 6.84 ISSQNFDD42 pKa = 4.16 LLEE45 pKa = 4.29 KK46 pKa = 10.66 VAVEE50 pKa = 4.36 AYY52 pKa = 9.72 KK53 pKa = 10.69 LGYY56 pKa = 9.4 EE57 pKa = 4.15 ATDD60 pKa = 3.65 PDD62 pKa = 4.57 EE63 pKa = 6.02 DD64 pKa = 4.28 VDD66 pKa = 3.9 DD67 pKa = 4.49 TGNVVIGFDD76 pKa = 3.36 IVAEE80 pKa = 4.26 SPLNAEE86 pKa = 5.42 LINAQIAEE94 pKa = 4.82 IITLTNQFGVNYY106 pKa = 9.22 DD107 pKa = 2.73 GWGTYY112 pKa = 10.12 FEE114 pKa = 5.88 DD115 pKa = 5.47 GSDD118 pKa = 4.31 DD119 pKa = 3.95 FNEE122 pKa = 4.04 VDD124 pKa = 5.19 DD125 pKa = 5.29 NMEE128 pKa = 4.12 TLHH131 pKa = 6.9
Molecular weight: 14.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.554
IPC_protein 3.541
Toseland 3.338
ProMoST 3.719
Dawson 3.541
Bjellqvist 3.706
Wikipedia 3.478
Rodwell 3.376
Grimsley 3.249
Solomon 3.528
Lehninger 3.478
Nozaki 3.668
DTASelect 3.872
Thurlkill 3.401
EMBOSS 3.49
Sillero 3.668
Patrickios 0.896
IPC_peptide 3.516
IPC2_peptide 3.643
IPC2.peptide.svr19 3.668
Protein with the highest isoelectric point:
>tr|A0A080K635|A0A080K635_9GAMM Uncharacterized protein (Fragment) OS=Gilliamella apicola SCGC AB-598-B02 OX=1445513 GN=GASC598B02_000450 PE=4 SV=1
MM1 pKa = 7.48 GKK3 pKa = 9.28 IFNTNYY9 pKa = 9.57 RR10 pKa = 11.84 AYY12 pKa = 9.38 GTRR15 pKa = 11.84 RR16 pKa = 11.84 LQAEE20 pKa = 4.28 LRR22 pKa = 11.84 KK23 pKa = 10.1 QGITVSRR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 IGRR35 pKa = 11.84 IMAKK39 pKa = 10.08 NGWVSKK45 pKa = 6.88 YY46 pKa = 7.4 TCKK49 pKa = 10.4 KK50 pKa = 9.37 YY51 pKa = 10.7 CIHH54 pKa = 6.13 TEE56 pKa = 3.77 KK57 pKa = 11.02 SNEE60 pKa = 4.09 SPVGNEE66 pKa = 3.74 LNRR69 pKa = 11.84 EE70 pKa = 4.03 FNIGQPRR77 pKa = 11.84 KK78 pKa = 9.91 ILVTDD83 pKa = 3.67 LTYY86 pKa = 11.18 VRR88 pKa = 11.84 VKK90 pKa = 10.53 QRR92 pKa = 11.84 WHH94 pKa = 5.38 YY95 pKa = 9.94 FCVIVDD101 pKa = 3.36 ISNRR105 pKa = 11.84 EE106 pKa = 3.64 IVGRR110 pKa = 11.84 SAGRR114 pKa = 11.84 HH115 pKa = 3.65 KK116 pKa = 9.51 TAALVMQAMSQIPMNLQTIEE136 pKa = 4.73 LFHH139 pKa = 7.14 SDD141 pKa = 3.49 RR142 pKa = 11.84 GNN144 pKa = 2.98
Molecular weight: 16.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.333
IPC2_protein 9.78
IPC_protein 10.467
Toseland 10.613
ProMoST 10.277
Dawson 10.73
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 11.038
Grimsley 10.789
Solomon 10.818
Lehninger 10.774
Nozaki 10.599
DTASelect 10.423
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.657
Patrickios 10.745
IPC_peptide 10.818
IPC2_peptide 9.428
IPC2.peptide.svr19 8.506
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1967
0
1967
494925
20
3876
251.6
28.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.907 ± 0.064
1.143 ± 0.02
5.376 ± 0.044
5.613 ± 0.058
4.45 ± 0.048
5.974 ± 0.068
2.075 ± 0.029
8.66 ± 0.057
6.689 ± 0.056
10.312 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.391 ± 0.032
5.76 ± 0.052
3.628 ± 0.035
4.496 ± 0.05
3.819 ± 0.042
6.432 ± 0.051
5.315 ± 0.032
6.063 ± 0.05
1.146 ± 0.021
3.75 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here