Moheibacter sediminis
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2974 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W2C911|A0A1W2C911_9FLAO Putative fluoride ion transporter CrcB OS=Moheibacter sediminis OX=1434700 GN=crcB PE=3 SV=1
MM1 pKa = 7.82 KK2 pKa = 9.9 IFNYY6 pKa = 9.37 ILSFIFISGLITSCSDD22 pKa = 4.21 DD23 pKa = 5.53 DD24 pKa = 4.0 FTKK27 pKa = 10.89 TNLNDD32 pKa = 3.63 ALKK35 pKa = 10.42 PSITSPSEE43 pKa = 3.66 GSSYY47 pKa = 11.43 EE48 pKa = 3.92 LLQEE52 pKa = 4.2 NAGNEE57 pKa = 4.1 AFTMEE62 pKa = 4.15 WEE64 pKa = 4.15 EE65 pKa = 4.26 ADD67 pKa = 3.9 FGVNSPVKK75 pKa = 10.3 YY76 pKa = 10.16 DD77 pKa = 3.55 VQASSSEE84 pKa = 4.18 DD85 pKa = 3.48 FSEE88 pKa = 4.22 TTNVITALTGTSTSLTVGRR107 pKa = 11.84 LNAVSLSSGLPFDD120 pKa = 4.23 VEE122 pKa = 4.31 GNLFIRR128 pKa = 11.84 VKK130 pKa = 10.61 AYY132 pKa = 10.77 LGVAGSSGFIYY143 pKa = 10.37 SEE145 pKa = 4.58 PISLNVTPYY154 pKa = 10.84 EE155 pKa = 3.92 PLIDD159 pKa = 5.57 LSTPWGMVGSAVPNGWEE176 pKa = 4.41 GPDD179 pKa = 3.31 VPFWKK184 pKa = 9.47 TDD186 pKa = 3.53 LNGIDD191 pKa = 3.56 DD192 pKa = 4.72 GKK194 pKa = 10.3 YY195 pKa = 9.18 VAYY198 pKa = 8.6 ATLTDD203 pKa = 3.62 GDD205 pKa = 3.64 IKK207 pKa = 10.61 IRR209 pKa = 11.84 KK210 pKa = 8.55 DD211 pKa = 3.45 NSWDD215 pKa = 3.49 EE216 pKa = 4.38 NYY218 pKa = 10.87 GGAAGTLVSNGDD230 pKa = 4.95 DD231 pKa = 3.27 IPMTAGTYY239 pKa = 10.1 KK240 pKa = 10.73 FNVDD244 pKa = 3.6 MNALTYY250 pKa = 10.47 EE251 pKa = 4.14 FEE253 pKa = 4.3 EE254 pKa = 4.59 YY255 pKa = 10.35 SWGIVGDD262 pKa = 3.8 ATPNGWNGPDD272 pKa = 3.42 VQLQYY277 pKa = 11.52 NGATNTWDD285 pKa = 3.61 AEE287 pKa = 4.54 VEE289 pKa = 4.28 FTNGNIKK296 pKa = 10.19 FRR298 pKa = 11.84 FNNAWALSYY307 pKa = 11.4 GDD309 pKa = 3.82 TGADD313 pKa = 3.07 GTLEE317 pKa = 4.19 SEE319 pKa = 4.38 NGADD323 pKa = 3.75 IPVTAGNYY331 pKa = 8.8 LVSVDD336 pKa = 4.41 FTNLTYY342 pKa = 10.68 SITPLL347 pKa = 3.45
Molecular weight: 37.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.695
IPC2_protein 3.732
IPC_protein 3.745
Toseland 3.516
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.427
Solomon 3.719
Lehninger 3.668
Nozaki 3.834
DTASelect 4.062
Thurlkill 3.567
EMBOSS 3.656
Sillero 3.859
Patrickios 1.189
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.773
Protein with the highest isoelectric point:
>tr|A0A1W2ATL0|A0A1W2ATL0_9FLAO Putative iron-regulated protein OS=Moheibacter sediminis OX=1434700 GN=SAMN06296427_10541 PE=4 SV=1
MM1 pKa = 7.54 SVRR4 pKa = 11.84 KK5 pKa = 9.68 LKK7 pKa = 10.5 PITPGQRR14 pKa = 11.84 FRR16 pKa = 11.84 IVNNFDD22 pKa = 3.19 NVEE25 pKa = 4.17 SVKK28 pKa = 10.43 PEE30 pKa = 3.49 KK31 pKa = 10.78 SLTVGKK37 pKa = 10.15 KK38 pKa = 9.23 RR39 pKa = 11.84 SGGRR43 pKa = 11.84 NHH45 pKa = 6.53 SGKK48 pKa = 8.3 MTMRR52 pKa = 11.84 YY53 pKa = 9.23 IGGGHH58 pKa = 5.49 KK59 pKa = 9.73 QSYY62 pKa = 9.64 RR63 pKa = 11.84 IVDD66 pKa = 4.1 FKK68 pKa = 11.27 RR69 pKa = 11.84 EE70 pKa = 4.05 KK71 pKa = 10.74 EE72 pKa = 4.26 GVATVDD78 pKa = 4.3 SIQYY82 pKa = 10.04 DD83 pKa = 3.71 PNRR86 pKa = 11.84 TAFIALIVYY95 pKa = 10.33 GDD97 pKa = 3.55 GEE99 pKa = 4.33 KK100 pKa = 10.32 RR101 pKa = 11.84 YY102 pKa = 10.71 VIAQNGLKK110 pKa = 10.12 KK111 pKa = 10.73 GQTIEE116 pKa = 4.0 SGEE119 pKa = 4.08 NVAPEE124 pKa = 3.74 IGNTLKK130 pKa = 10.95 LKK132 pKa = 10.04 NVPLGTVISCIEE144 pKa = 3.9 LRR146 pKa = 11.84 PGQGALMARR155 pKa = 11.84 SAGSYY160 pKa = 9.0 AQLVARR166 pKa = 11.84 DD167 pKa = 3.78 GKK169 pKa = 10.37 YY170 pKa = 9.12 ATVKK174 pKa = 10.47 LPSGEE179 pKa = 3.88 SRR181 pKa = 11.84 MILVEE186 pKa = 4.29 CKK188 pKa = 9.45 ATIGVVSNSDD198 pKa = 3.13 HH199 pKa = 5.64 QLEE202 pKa = 4.55 VSGKK206 pKa = 10.07 AGRR209 pKa = 11.84 TRR211 pKa = 11.84 WKK213 pKa = 9.99 GRR215 pKa = 11.84 RR216 pKa = 11.84 PRR218 pKa = 11.84 TRR220 pKa = 11.84 PMVMNPVDD228 pKa = 3.52 HH229 pKa = 7.07 PMGGGEE235 pKa = 3.92 GRR237 pKa = 11.84 ATGGIPRR244 pKa = 11.84 SRR246 pKa = 11.84 NGQPSKK252 pKa = 10.7 GYY254 pKa = 8.28 KK255 pKa = 8.33 TRR257 pKa = 11.84 APKK260 pKa = 8.88 KK261 pKa = 7.78 TSSKK265 pKa = 10.57 YY266 pKa = 8.93 IVEE269 pKa = 4.17 RR270 pKa = 11.84 RR271 pKa = 11.84 KK272 pKa = 10.24 KK273 pKa = 10.1
Molecular weight: 29.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.294
IPC2_protein 9.78
IPC_protein 10.277
Toseland 10.76
ProMoST 10.365
Dawson 10.847
Bjellqvist 10.496
Wikipedia 11.008
Rodwell 11.272
Grimsley 10.891
Solomon 10.921
Lehninger 10.891
Nozaki 10.73
DTASelect 10.496
Thurlkill 10.745
EMBOSS 11.155
Sillero 10.774
Patrickios 10.965
IPC_peptide 10.935
IPC2_peptide 9.18
IPC2.peptide.svr19 8.684
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2974
0
2974
985835
28
3536
331.5
37.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.889 ± 0.047
0.7 ± 0.015
5.413 ± 0.035
7.13 ± 0.046
5.412 ± 0.033
6.498 ± 0.049
1.696 ± 0.019
8.204 ± 0.043
7.651 ± 0.066
9.064 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.28 ± 0.02
6.588 ± 0.048
3.323 ± 0.027
3.623 ± 0.022
3.223 ± 0.029
6.646 ± 0.037
5.513 ± 0.045
5.926 ± 0.036
1.069 ± 0.017
4.152 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here