Cucumis melo var. makuwa

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis; Cucumis melo; Cucumi

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37791 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5A7THJ8|A0A5A7THJ8_CUCME Pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G001980 PE=4 SV=1
MM1 pKa = 7.56FLTIHH6 pKa = 7.29LILPLKK12 pKa = 9.57WEE14 pKa = 4.56AYY16 pKa = 3.97WTKK19 pKa = 10.96LMSCPQYY26 pKa = 10.62VDD28 pKa = 4.09LRR30 pKa = 11.84IISCEE35 pKa = 3.78QEE37 pKa = 3.82FTVCSRR43 pKa = 11.84LRR45 pKa = 11.84LSYY48 pKa = 10.42LVKK51 pKa = 10.65DD52 pKa = 4.03DD53 pKa = 4.46LLDD56 pKa = 3.95YY57 pKa = 11.23GHH59 pKa = 7.25IEE61 pKa = 4.18YY62 pKa = 10.83EE63 pKa = 4.19EE64 pKa = 5.78GDD66 pKa = 4.09DD67 pKa = 4.14PFGSAAFKK75 pKa = 10.32MKK77 pKa = 10.32IKK79 pKa = 10.36IILSTATILKK89 pKa = 9.09TIEE92 pKa = 3.96EE93 pKa = 4.42EE94 pKa = 4.1NEE96 pKa = 3.75KK97 pKa = 11.12DD98 pKa = 5.26LFSCDD103 pKa = 3.76YY104 pKa = 11.05IEE106 pKa = 6.51DD107 pKa = 3.64EE108 pKa = 5.75DD109 pKa = 4.79NPLDD113 pKa = 4.15CGSIEE118 pKa = 5.98DD119 pKa = 4.39YY120 pKa = 11.52DD121 pKa = 4.33EE122 pKa = 6.51DD123 pKa = 6.1DD124 pKa = 4.42PFCCGRR130 pKa = 11.84IEE132 pKa = 5.82DD133 pKa = 4.04EE134 pKa = 5.08DD135 pKa = 5.81GYY137 pKa = 11.62DD138 pKa = 4.38PLCCDD143 pKa = 5.29DD144 pKa = 6.45LLDD147 pKa = 4.11CSRR150 pKa = 11.84NEE152 pKa = 5.42DD153 pKa = 3.8EE154 pKa = 5.82NKK156 pKa = 10.63DD157 pKa = 3.44DD158 pKa = 4.23ALNYY162 pKa = 10.95GCMEE166 pKa = 5.0DD167 pKa = 4.8KK168 pKa = 10.71DD169 pKa = 5.18DD170 pKa = 4.69PFDD173 pKa = 3.88YY174 pKa = 11.19SHH176 pKa = 8.15IEE178 pKa = 5.2DD179 pKa = 4.45EE180 pKa = 6.06DD181 pKa = 4.15EE182 pKa = 5.26DD183 pKa = 5.29DD184 pKa = 4.05PLGYY188 pKa = 10.7VCIEE192 pKa = 5.04DD193 pKa = 4.06EE194 pKa = 6.02DD195 pKa = 4.67EE196 pKa = 6.36DD197 pKa = 4.83DD198 pKa = 6.35LLDD201 pKa = 4.02EE202 pKa = 4.75DD203 pKa = 5.37HH204 pKa = 7.6PLGCDD209 pKa = 3.44CIEE212 pKa = 4.85NKK214 pKa = 10.54DD215 pKa = 3.98DD216 pKa = 6.05DD217 pKa = 5.44DD218 pKa = 4.99PLICDD223 pKa = 4.66RR224 pKa = 11.84IKK226 pKa = 11.35DD227 pKa = 3.93EE228 pKa = 6.76DD229 pKa = 3.87DD230 pKa = 4.41LFYY233 pKa = 10.95CGCIEE238 pKa = 6.01DD239 pKa = 4.25EE240 pKa = 5.36DD241 pKa = 4.61EE242 pKa = 5.81DD243 pKa = 5.14GPLDD247 pKa = 3.93FVLIKK252 pKa = 10.93DD253 pKa = 3.72KK254 pKa = 11.55DD255 pKa = 3.95EE256 pKa = 4.64NDD258 pKa = 3.72PLSCGRR264 pKa = 11.84IEE266 pKa = 4.53VEE268 pKa = 5.03FDD270 pKa = 3.25PFCYY274 pKa = 10.13DD275 pKa = 4.38HH276 pKa = 7.08EE277 pKa = 5.38GKK279 pKa = 10.4DD280 pKa = 3.41EE281 pKa = 5.24DD282 pKa = 5.42DD283 pKa = 4.06PFGCRR288 pKa = 11.84RR289 pKa = 11.84IDD291 pKa = 4.66DD292 pKa = 3.85EE293 pKa = 6.42HH294 pKa = 7.6EE295 pKa = 4.34DD296 pKa = 4.25ASLNLDD302 pKa = 4.14NIKK305 pKa = 10.89DD306 pKa = 3.76EE307 pKa = 4.43YY308 pKa = 11.54DD309 pKa = 3.43PLDD312 pKa = 3.49YY313 pKa = 11.24DD314 pKa = 4.41RR315 pKa = 11.84IEE317 pKa = 6.06DD318 pKa = 3.94EE319 pKa = 6.51DD320 pKa = 4.69EE321 pKa = 5.07DD322 pKa = 5.13DD323 pKa = 4.61PLNIDD328 pKa = 3.58RR329 pKa = 11.84SEE331 pKa = 4.67DD332 pKa = 3.52KK333 pKa = 10.94DD334 pKa = 4.66DD335 pKa = 4.12NDD337 pKa = 4.17PLGFRR342 pKa = 11.84CIKK345 pKa = 10.95DD346 pKa = 3.65EE347 pKa = 4.37VDD349 pKa = 3.23DD350 pKa = 3.86TLGFGRR356 pKa = 11.84IKK358 pKa = 11.1DD359 pKa = 4.02EE360 pKa = 4.9DD361 pKa = 4.15DD362 pKa = 5.06HH363 pKa = 8.79PLDD366 pKa = 4.08CNKK369 pKa = 9.94WEE371 pKa = 4.04MLKK374 pKa = 10.95LLACNISMRR383 pKa = 11.84SRR385 pKa = 11.84IQQ387 pKa = 2.94

Molecular weight: 45.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5A7SN37|A0A5A7SN37_CUCME Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold581G00420 PE=4 SV=1
MM1 pKa = 7.57RR2 pKa = 11.84ASFKK6 pKa = 9.38STRR9 pKa = 11.84LMRR12 pKa = 11.84ASFGSTRR19 pKa = 11.84LMRR22 pKa = 11.84ASFGSTRR29 pKa = 11.84LMRR32 pKa = 11.84ASFGSTRR39 pKa = 11.84LMRR42 pKa = 11.84ASFGSTRR49 pKa = 11.84LMRR52 pKa = 11.84ASFGSTRR59 pKa = 11.84LMRR62 pKa = 11.84ASFGSTRR69 pKa = 11.84LMRR72 pKa = 11.84ASFGSTRR79 pKa = 11.84LMRR82 pKa = 11.84ASFGSTRR89 pKa = 11.84LMRR92 pKa = 11.84ASFGSTRR99 pKa = 11.84LMRR102 pKa = 11.84ASFGSTRR109 pKa = 11.84LMRR112 pKa = 11.84ASFGSTRR119 pKa = 11.84LMRR122 pKa = 11.84ASFGSTRR129 pKa = 11.84LMRR132 pKa = 11.84ASFGSTRR139 pKa = 11.84LMRR142 pKa = 11.84ASFGSTRR149 pKa = 11.84LMRR152 pKa = 11.84ASFGSTRR159 pKa = 11.84LMRR162 pKa = 11.84ASFGSTRR169 pKa = 11.84LMRR172 pKa = 11.84ASFGSTRR179 pKa = 11.84LMRR182 pKa = 11.84ASFGSTRR189 pKa = 11.84LMRR192 pKa = 11.84ASFGSTRR199 pKa = 11.84LMRR202 pKa = 11.84ASFGSTRR209 pKa = 11.84LMRR212 pKa = 11.84ASFGSTRR219 pKa = 11.84LMRR222 pKa = 11.84ASFGSTRR229 pKa = 11.84LMRR232 pKa = 11.84ASFGSTRR239 pKa = 11.84LICASFGSTRR249 pKa = 11.84LICAFYY255 pKa = 8.81GTTRR259 pKa = 11.84LLFKK263 pKa = 9.78GTARR267 pKa = 11.84GRR269 pKa = 11.84PTRR272 pKa = 11.84GRR274 pKa = 11.84KK275 pKa = 8.91DD276 pKa = 2.97AA277 pKa = 4.69

Molecular weight: 30.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

37791

0

37791

14011924

35

5438

370.8

41.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.215 ± 0.011

1.849 ± 0.006

5.333 ± 0.009

6.63 ± 0.015

4.336 ± 0.009

6.03 ± 0.01

2.451 ± 0.005

5.433 ± 0.01

6.504 ± 0.014

9.505 ± 0.012

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.5 ± 0.006

4.327 ± 0.01

4.822 ± 0.011

3.712 ± 0.008

5.726 ± 0.01

8.616 ± 0.014

5.111 ± 0.008

6.688 ± 0.01

1.351 ± 0.004

2.857 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski