Amino acid dipepetide frequency for Streptomyces sp. sk2.1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.903AlaAla: 20.903 ± 0.154	1.051AlaCys: 1.051 ± 0.021	8.505AlaAsp: 8.505 ± 0.059	8.797AlaGlu: 8.797 ± 0.081	3.431AlaPhe: 3.431 ± 0.041	13.242AlaGly: 13.242 ± 0.09	2.921AlaHis: 2.921 ± 0.033	3.28AlaIle: 3.28 ± 0.042	2.565AlaLys: 2.565 ± 0.042	14.374AlaLeu: 14.374 ± 0.102	2.52AlaMet: 2.52 ± 0.034	1.792AlaAsn: 1.792 ± 0.027	7.412AlaPro: 7.412 ± 0.067	3.345AlaGln: 3.345 ± 0.044	10.755AlaArg: 10.755 ± 0.073	5.633AlaSer: 5.633 ± 0.048	6.837AlaThr: 6.837 ± 0.05	12.212AlaVal: 12.212 ± 0.087	1.801AlaTrp: 1.801 ± 0.027	2.561AlaTyr: 2.561 ± 0.032	0.008AlaXaa: 0.008 ± 0.002
Cys	1.136CysAla: 1.136 ± 0.021	0.098CysCys: 0.098 ± 0.007	0.491CysAsp: 0.491 ± 0.015	0.406CysGlu: 0.406 ± 0.012	0.231CysPhe: 0.231 ± 0.011	0.983CysGly: 0.983 ± 0.019	0.199CysHis: 0.199 ± 0.009	0.16CysIle: 0.16 ± 0.007	0.111CysLys: 0.111 ± 0.007	0.777CysLeu: 0.777 ± 0.018	0.129CysMet: 0.129 ± 0.007	0.135CysAsn: 0.135 ± 0.008	0.523CysPro: 0.523 ± 0.016	0.145CysGln: 0.145 ± 0.007	0.672CysArg: 0.672 ± 0.017	0.471CysSer: 0.471 ± 0.014	0.522CysThr: 0.522 ± 0.015	0.672CysVal: 0.672 ± 0.015	0.129CysTrp: 0.129 ± 0.008	0.154CysTyr: 0.154 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	7.746AspAla: 7.746 ± 0.067	0.455AspCys: 0.455 ± 0.014	3.811AspAsp: 3.811 ± 0.047	4.128AspGlu: 4.128 ± 0.044	1.641AspPhe: 1.641 ± 0.023	6.981AspGly: 6.981 ± 0.058	1.482AspHis: 1.482 ± 0.027	1.903AspIle: 1.903 ± 0.026	1.097AspLys: 1.097 ± 0.027	6.187AspLeu: 6.187 ± 0.053	0.832AspMet: 0.832 ± 0.019	0.924AspAsn: 0.924 ± 0.022	4.716AspPro: 4.716 ± 0.051	1.349AspGln: 1.349 ± 0.025	5.671AspArg: 5.671 ± 0.053	2.535AspSer: 2.535 ± 0.032	3.317AspThr: 3.317 ± 0.038	4.586AspVal: 4.586 ± 0.048	1.052AspTrp: 1.052 ± 0.023	1.062AspTyr: 1.062 ± 0.022	0.003AspXaa: 0.003 ± 0.001
Glu	7.255GluAla: 7.255 ± 0.067	0.396GluCys: 0.396 ± 0.012	2.896GluAsp: 2.896 ± 0.035	3.736GluGlu: 3.736 ± 0.048	1.469GluPhe: 1.469 ± 0.025	4.38GluGly: 4.38 ± 0.044	1.595GluHis: 1.595 ± 0.027	2.245GluIle: 2.245 ± 0.033	1.445GluLys: 1.445 ± 0.022	7.073GluLeu: 7.073 ± 0.063	0.856GluMet: 0.856 ± 0.018	1.098GluAsn: 1.098 ± 0.023	3.503GluPro: 3.503 ± 0.039	2.247GluGln: 2.247 ± 0.033	5.736GluArg: 5.736 ± 0.056	2.626GluSer: 2.626 ± 0.033	2.871GluThr: 2.871 ± 0.035	4.439GluVal: 4.439 ± 0.048	0.784GluTrp: 0.784 ± 0.016	1.142GluTyr: 1.142 ± 0.023	0.004GluXaa: 0.004 ± 0.001
Phe	3.581PheAla: 3.581 ± 0.038	0.283PheCys: 0.283 ± 0.01	2.037PheAsp: 2.037 ± 0.026	1.479PheGlu: 1.479 ± 0.026	0.856PhePhe: 0.856 ± 0.02	3.009PheGly: 3.009 ± 0.034	0.606PheHis: 0.606 ± 0.017	0.696PheIle: 0.696 ± 0.018	0.455PheLys: 0.455 ± 0.015	2.54PheLeu: 2.54 ± 0.034	0.394PheMet: 0.394 ± 0.011	0.57PheAsn: 0.57 ± 0.015	1.435PhePro: 1.435 ± 0.023	0.626PheGln: 0.626 ± 0.015	1.957PheArg: 1.957 ± 0.027	1.426PheSer: 1.426 ± 0.025	1.931PheThr: 1.931 ± 0.028	2.246PheVal: 2.246 ± 0.031	0.423PheTrp: 0.423 ± 0.013	0.535PheTyr: 0.535 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	11.541GlyAla: 11.541 ± 0.079	0.867GlyCys: 0.867 ± 0.02	5.325GlyAsp: 5.325 ± 0.049	5.232GlyGlu: 5.232 ± 0.053	2.894GlyPhe: 2.894 ± 0.033	9.051GlyGly: 9.051 ± 0.08	2.405GlyHis: 2.405 ± 0.032	3.606GlyIle: 3.606 ± 0.042	2.392GlyLys: 2.392 ± 0.034	9.566GlyLeu: 9.566 ± 0.069	2.016GlyMet: 2.016 ± 0.029	1.848GlyAsn: 1.848 ± 0.034	5.786GlyPro: 5.786 ± 0.055	2.427GlyGln: 2.427 ± 0.033	8.481GlyArg: 8.481 ± 0.075	5.569GlySer: 5.569 ± 0.055	6.879GlyThr: 6.879 ± 0.056	7.419GlyVal: 7.419 ± 0.066	1.654GlyTrp: 1.654 ± 0.028	2.195GlyTyr: 2.195 ± 0.03	0.008GlyXaa: 0.008 ± 0.002
His	2.609HisAla: 2.609 ± 0.029	0.219HisCys: 0.219 ± 0.009	1.462HisAsp: 1.462 ± 0.022	1.287HisGlu: 1.287 ± 0.02	0.635HisPhe: 0.635 ± 0.017	2.497HisGly: 2.497 ± 0.032	0.724HisHis: 0.724 ± 0.016	0.69HisIle: 0.69 ± 0.018	0.309HisLys: 0.309 ± 0.01	2.41HisLeu: 2.41 ± 0.033	0.344HisMet: 0.344 ± 0.012	0.364HisAsn: 0.364 ± 0.013	1.862HisPro: 1.862 ± 0.029	0.619HisGln: 0.619 ± 0.014	2.34HisArg: 2.34 ± 0.031	1.022HisSer: 1.022 ± 0.02	1.332HisThr: 1.332 ± 0.024	1.669HisVal: 1.669 ± 0.024	0.383HisTrp: 0.383 ± 0.012	0.479HisTyr: 0.479 ± 0.013	0.001HisXaa: 0.001 ± 0.001
Ile	4.666IleAla: 4.666 ± 0.045	0.306IleCys: 0.306 ± 0.012	2.353IleAsp: 2.353 ± 0.035	2.048IleGlu: 2.048 ± 0.033	0.658IlePhe: 0.658 ± 0.016	3.757IleGly: 3.757 ± 0.045	0.642IleHis: 0.642 ± 0.016	0.818IleIle: 0.818 ± 0.022	0.703IleLys: 0.703 ± 0.019	2.267IleLeu: 2.267 ± 0.034	0.415IleMet: 0.415 ± 0.012	0.664IleAsn: 0.664 ± 0.017	1.677IlePro: 1.677 ± 0.025	0.665IleGln: 0.665 ± 0.017	2.296IleArg: 2.296 ± 0.033	1.64IleSer: 1.64 ± 0.03	2.192IleThr: 2.192 ± 0.031	2.676IleVal: 2.676 ± 0.039	0.387IleTrp: 0.387 ± 0.014	0.476IleTyr: 0.476 ± 0.014	0.0IleXaa: 0.0 ± 0.0
Lys	2.583LysAla: 2.583 ± 0.04	0.109LysCys: 0.109 ± 0.007	1.32LysAsp: 1.32 ± 0.026	1.202LysGlu: 1.202 ± 0.025	0.452LysPhe: 0.452 ± 0.014	1.786LysGly: 1.786 ± 0.03	0.441LysHis: 0.441 ± 0.014	0.793LysIle: 0.793 ± 0.02	0.898LysLys: 0.898 ± 0.032	1.907LysLeu: 1.907 ± 0.03	0.374LysMet: 0.374 ± 0.013	0.533LysAsn: 0.533 ± 0.018	1.282LysPro: 1.282 ± 0.024	0.658LysGln: 0.658 ± 0.019	1.364LysArg: 1.364 ± 0.025	1.132LysSer: 1.132 ± 0.024	1.197LysThr: 1.197 ± 0.026	1.723LysVal: 1.723 ± 0.03	0.261LysTrp: 0.261 ± 0.009	0.448LysTyr: 0.448 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	14.809LeuAla: 14.809 ± 0.112	0.898LeuCys: 0.898 ± 0.022	6.991LeuAsp: 6.991 ± 0.058	4.657LeuGlu: 4.657 ± 0.047	2.64LeuPhe: 2.64 ± 0.034	9.26LeuGly: 9.26 ± 0.068	2.304LeuHis: 2.304 ± 0.037	3.208LeuIle: 3.208 ± 0.04	1.888LeuLys: 1.888 ± 0.036	11.329LeuLeu: 11.329 ± 0.098	1.652LeuMet: 1.652 ± 0.027	1.629LeuAsn: 1.629 ± 0.024	6.522LeuPro: 6.522 ± 0.063	2.012LeuGln: 2.012 ± 0.034	8.939LeuArg: 8.939 ± 0.067	5.278LeuSer: 5.278 ± 0.047	6.729LeuThr: 6.729 ± 0.056	8.914LeuVal: 8.914 ± 0.067	1.297LeuTrp: 1.297 ± 0.022	1.768LeuTyr: 1.768 ± 0.027	0.003LeuXaa: 0.003 ± 0.001
Met	2.201MetAla: 2.201 ± 0.032	0.146MetCys: 0.146 ± 0.008	0.898MetAsp: 0.898 ± 0.02	0.773MetGlu: 0.773 ± 0.018	0.455MetPhe: 0.455 ± 0.012	1.346MetGly: 1.346 ± 0.027	0.347MetHis: 0.347 ± 0.013	0.666MetIle: 0.666 ± 0.017	0.425MetLys: 0.425 ± 0.011	1.697MetLeu: 1.697 ± 0.026	0.303MetMet: 0.303 ± 0.012	0.443MetAsn: 0.443 ± 0.013	1.132MetPro: 1.132 ± 0.021	0.416MetGln: 0.416 ± 0.015	1.439MetArg: 1.439 ± 0.021	1.337MetSer: 1.337 ± 0.024	1.504MetThr: 1.504 ± 0.026	1.283MetVal: 1.283 ± 0.026	0.208MetTrp: 0.208 ± 0.009	0.33MetTyr: 0.33 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.121AsnAla: 2.121 ± 0.027	0.163AsnCys: 0.163 ± 0.008	0.938AsnAsp: 0.938 ± 0.022	0.813AsnGlu: 0.813 ± 0.019	0.467AsnPhe: 0.467 ± 0.013	1.935AsnGly: 1.935 ± 0.031	0.401AsnHis: 0.401 ± 0.014	0.62AsnIle: 0.62 ± 0.014	0.398AsnLys: 0.398 ± 0.013	1.594AsnLeu: 1.594 ± 0.025	0.293AsnMet: 0.293 ± 0.011	0.423AsnAsn: 0.423 ± 0.013	1.336AsnPro: 1.336 ± 0.023	0.46AsnGln: 0.46 ± 0.013	1.361AsnArg: 1.361 ± 0.022	0.894AsnSer: 0.894 ± 0.021	1.168AsnThr: 1.168 ± 0.024	1.304AsnVal: 1.304 ± 0.025	0.284AsnTrp: 0.284 ± 0.01	0.392AsnTyr: 0.392 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	8.83ProAla: 8.83 ± 0.068	0.368ProCys: 0.368 ± 0.013	4.886ProAsp: 4.886 ± 0.053	4.474ProGlu: 4.474 ± 0.047	1.553ProPhe: 1.553 ± 0.025	7.128ProGly: 7.128 ± 0.062	1.394ProHis: 1.394 ± 0.024	1.218ProIle: 1.218 ± 0.024	1.137ProLys: 1.137 ± 0.027	5.366ProLeu: 5.366 ± 0.054	1.033ProMet: 1.033 ± 0.02	0.86ProAsn: 0.86 ± 0.019	3.492ProPro: 3.492 ± 0.058	1.628ProGln: 1.628 ± 0.03	4.117ProArg: 4.117 ± 0.046	3.158ProSer: 3.158 ± 0.038	3.07ProThr: 3.07 ± 0.036	5.925ProVal: 5.925 ± 0.052	0.906ProTrp: 0.906 ± 0.021	1.38ProTyr: 1.38 ± 0.023	0.006ProXaa: 0.006 ± 0.001
Gln	3.164GlnAla: 3.164 ± 0.038	0.157GlnCys: 0.157 ± 0.008	1.369GlnAsp: 1.369 ± 0.022	1.432GlnGlu: 1.432 ± 0.025	0.616GlnPhe: 0.616 ± 0.017	2.116GlnGly: 2.116 ± 0.031	0.631GlnHis: 0.631 ± 0.015	1.01GlnIle: 1.01 ± 0.02	0.559GlnLys: 0.559 ± 0.017	2.794GlnLeu: 2.794 ± 0.035	0.447GlnMet: 0.447 ± 0.013	0.477GlnAsn: 0.477 ± 0.015	1.494GlnPro: 1.494 ± 0.027	1.155GlnGln: 1.155 ± 0.031	2.201GlnArg: 2.201 ± 0.032	1.181GlnSer: 1.181 ± 0.024	1.185GlnThr: 1.185 ± 0.025	2.155GlnVal: 2.155 ± 0.025	0.451GlnTrp: 0.451 ± 0.014	0.584GlnTyr: 0.584 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	10.403ArgAla: 10.403 ± 0.088	0.669ArgCys: 0.669 ± 0.018	4.456ArgAsp: 4.456 ± 0.054	4.886ArgGlu: 4.886 ± 0.043	2.419ArgPhe: 2.419 ± 0.036	6.242ArgGly: 6.242 ± 0.06	2.168ArgHis: 2.168 ± 0.027	3.482ArgIle: 3.482 ± 0.038	1.695ArgLys: 1.695 ± 0.029	9.121ArgLeu: 9.121 ± 0.072	1.78ArgMet: 1.78 ± 0.026	1.473ArgAsn: 1.473 ± 0.027	5.29ArgPro: 5.29 ± 0.052	2.153ArgGln: 2.153 ± 0.029	8.168ArgArg: 8.168 ± 0.078	4.296ArgSer: 4.296 ± 0.04	6.102ArgThr: 6.102 ± 0.056	6.001ArgVal: 6.001 ± 0.053	1.383ArgTrp: 1.383 ± 0.023	1.749ArgTyr: 1.749 ± 0.026	0.005ArgXaa: 0.005 ± 0.001
Ser	6.611SerAla: 6.611 ± 0.06	0.429SerCys: 0.429 ± 0.012	2.705SerAsp: 2.705 ± 0.036	2.34SerGlu: 2.34 ± 0.034	1.607SerPhe: 1.607 ± 0.027	6.228SerGly: 6.228 ± 0.061	1.037SerHis: 1.037 ± 0.021	1.434SerIle: 1.434 ± 0.024	0.952SerLys: 0.952 ± 0.023	4.724SerLeu: 4.724 ± 0.045	1.071SerMet: 1.071 ± 0.021	0.849SerAsn: 0.849 ± 0.019	3.086SerPro: 3.086 ± 0.033	1.115SerGln: 1.115 ± 0.022	3.799SerArg: 3.799 ± 0.039	2.836SerSer: 2.836 ± 0.044	3.0SerThr: 3.0 ± 0.034	4.31SerVal: 4.31 ± 0.035	0.923SerTrp: 0.923 ± 0.019	1.182SerTyr: 1.182 ± 0.024	0.002SerXaa: 0.002 ± 0.001
Thr	9.04ThrAla: 9.04 ± 0.072	0.423ThrCys: 0.423 ± 0.013	3.857ThrAsp: 3.857 ± 0.049	3.327ThrGlu: 3.327 ± 0.039	1.553ThrPhe: 1.553 ± 0.027	7.447ThrGly: 7.447 ± 0.058	1.21ThrHis: 1.21 ± 0.02	1.682ThrIle: 1.682 ± 0.028	1.085ThrLys: 1.085 ± 0.024	5.505ThrLeu: 5.505 ± 0.053	0.951ThrMet: 0.951 ± 0.017	0.965ThrAsn: 0.965 ± 0.02	4.017ThrPro: 4.017 ± 0.045	1.147ThrGln: 1.147 ± 0.019	4.004ThrArg: 4.004 ± 0.04	3.095ThrSer: 3.095 ± 0.034	3.904ThrThr: 3.904 ± 0.053	6.123ThrVal: 6.123 ± 0.056	0.865ThrTrp: 0.865 ± 0.02	1.263ThrTyr: 1.263 ± 0.027	0.003ThrXaa: 0.003 ± 0.001
Val	10.53ValAla: 10.53 ± 0.074	0.759ValCys: 0.759 ± 0.018	4.922ValAsp: 4.922 ± 0.05	4.785ValGlu: 4.785 ± 0.047	2.404ValPhe: 2.404 ± 0.033	6.557ValGly: 6.557 ± 0.059	2.005ValHis: 2.005 ± 0.032	2.758ValIle: 2.758 ± 0.035	1.631ValLys: 1.631 ± 0.027	9.739ValLeu: 9.739 ± 0.078	1.406ValMet: 1.406 ± 0.024	1.542ValAsn: 1.542 ± 0.024	5.535ValPro: 5.535 ± 0.049	1.946ValGln: 1.946 ± 0.028	7.368ValArg: 7.368 ± 0.061	4.217ValSer: 4.217 ± 0.046	5.47ValThr: 5.47 ± 0.056	7.972ValVal: 7.972 ± 0.069	1.107ValTrp: 1.107 ± 0.024	1.498ValTyr: 1.498 ± 0.023	0.002ValXaa: 0.002 ± 0.001
Trp	1.645TrpAla: 1.645 ± 0.025	0.165TrpCys: 0.165 ± 0.008	0.858TrpAsp: 0.858 ± 0.018	0.748TrpGlu: 0.748 ± 0.018	0.491TrpPhe: 0.491 ± 0.016	1.005TrpGly: 1.005 ± 0.023	0.371TrpHis: 0.371 ± 0.014	0.569TrpIle: 0.569 ± 0.015	0.372TrpLys: 0.372 ± 0.011	1.715TrpLeu: 1.715 ± 0.027	0.277TrpMet: 0.277 ± 0.01	0.417TrpAsn: 0.417 ± 0.012	0.779TrpPro: 0.779 ± 0.019	0.563TrpGln: 0.563 ± 0.016	1.342TrpArg: 1.342 ± 0.025	0.984TrpSer: 0.984 ± 0.022	1.058TrpThr: 1.058 ± 0.021	0.942TrpVal: 0.942 ± 0.02	0.344TrpTrp: 0.344 ± 0.014	0.35TrpTyr: 0.35 ± 0.013	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.565TyrAla: 2.565 ± 0.033	0.184TyrCys: 0.184 ± 0.008	1.396TyrAsp: 1.396 ± 0.028	1.242TyrGlu: 1.242 ± 0.026	0.612TyrPhe: 0.612 ± 0.016	2.202TyrGly: 2.202 ± 0.036	0.372TyrHis: 0.372 ± 0.01	0.466TyrIle: 0.466 ± 0.014	0.349TyrLys: 0.349 ± 0.012	2.039TyrLeu: 2.039 ± 0.027	0.257TyrMet: 0.257 ± 0.011	0.358TyrAsn: 0.358 ± 0.013	1.047TyrPro: 1.047 ± 0.022	0.501TyrGln: 0.501 ± 0.014	1.925TyrArg: 1.925 ± 0.03	0.909TyrSer: 0.909 ± 0.021	1.196TyrThr: 1.196 ± 0.026	1.576TyrVal: 1.576 ± 0.025	0.354TyrTrp: 0.354 ± 0.012	0.413TyrTyr: 0.413 ± 0.015	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.008XaaAla: 0.008 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.002XaaAsp: 0.002 ± 0.001	0.003XaaGlu: 0.003 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.007XaaGly: 0.007 ± 0.002	0.002XaaHis: 0.002 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.005XaaLeu: 0.005 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.001XaaAsn: 0.001 ± 0.0	0.003XaaPro: 0.003 ± 0.001	0.002XaaGln: 0.002 ± 0.001	0.007XaaArg: 0.007 ± 0.002	0.002XaaSer: 0.002 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.002XaaVal: 0.002 ± 0.001	0.002XaaTrp: 0.002 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.003XaaXaa: 0.003 ± 0.001

Statistics based on 8051 proteins (2631059 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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