Ignavibacterium album (strain DSM 19864 / JCM 16511 / NBRC 101810 / Mat9-16)
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3169 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I0AIB4|I0AIB4_IGNAJ Uncharacterized protein OS=Ignavibacterium album (strain DSM 19864 / JCM 16511 / NBRC 101810 / Mat9-16) OX=945713 GN=IALB_1010 PE=4 SV=1
MM1 pKa = 7.5 KK2 pKa = 10.55 FINLYY7 pKa = 9.73 IAILIITTSANAEE20 pKa = 4.0 VDD22 pKa = 3.37 SLTIIRR28 pKa = 11.84 KK29 pKa = 9.34 DD30 pKa = 3.84 FYY32 pKa = 11.1 QATDD36 pKa = 3.36 DD37 pKa = 3.32 TSAYY41 pKa = 10.79 VKK43 pKa = 10.22 ILYY46 pKa = 8.4 PQINGLDD53 pKa = 3.52 NPEE56 pKa = 3.99 VQKK59 pKa = 10.93 KK60 pKa = 9.96 INSFLEE66 pKa = 4.3 TEE68 pKa = 4.64 FMQAKK73 pKa = 9.44 SWFDD77 pKa = 3.7 DD78 pKa = 4.61 FVADD82 pKa = 3.62 TDD84 pKa = 4.13 YY85 pKa = 11.44 TSEE88 pKa = 4.89 FPPDD92 pKa = 2.99 WVFSFEE98 pKa = 3.82 TDD100 pKa = 3.74 FNVTYY105 pKa = 11.05 NSIEE109 pKa = 4.02 FLSIVLNYY117 pKa = 10.77 YY118 pKa = 9.9 EE119 pKa = 4.44 FTGGAHH125 pKa = 6.0 GNYY128 pKa = 9.89 YY129 pKa = 10.46 SVGYY133 pKa = 10.0 NIRR136 pKa = 11.84 TSDD139 pKa = 4.05 GEE141 pKa = 4.4 VLILPDD147 pKa = 3.34 ILKK150 pKa = 10.37 PNSLQALSEE159 pKa = 4.24 FCTEE163 pKa = 4.55 EE164 pKa = 3.92 ILNMFDD170 pKa = 4.5 ANSLNEE176 pKa = 3.99 AGLFEE181 pKa = 5.64 DD182 pKa = 4.99 EE183 pKa = 5.64 LNISDD188 pKa = 5.14 DD189 pKa = 3.34 QDD191 pKa = 4.37 FYY193 pKa = 10.83 ITPDD197 pKa = 3.36 ALVLQFDD204 pKa = 4.5 PYY206 pKa = 10.78 EE207 pKa = 4.16 IAPYY211 pKa = 11.15 AMGSIDD217 pKa = 4.41 VEE219 pKa = 4.54 LKK221 pKa = 10.12 FSKK224 pKa = 10.17 IKK226 pKa = 10.92 NILKK230 pKa = 10.4 EE231 pKa = 3.93 NLPFLNRR238 pKa = 3.63
Molecular weight: 27.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.91
IPC_protein 3.897
Toseland 3.694
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.037
Wikipedia 3.795
Rodwell 3.719
Grimsley 3.605
Solomon 3.872
Lehninger 3.821
Nozaki 3.986
DTASelect 4.202
Thurlkill 3.732
EMBOSS 3.808
Sillero 4.012
Patrickios 1.901
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.909
Protein with the highest isoelectric point:
>tr|I0ALU3|I0ALU3_IGNAJ Serine phosphatase RsbU OS=Ignavibacterium album (strain DSM 19864 / JCM 16511 / NBRC 101810 / Mat9-16) OX=945713 GN=IALB_2247 PE=4 SV=1
MM1 pKa = 7.32 PCGRR5 pKa = 11.84 KK6 pKa = 9.29 RR7 pKa = 11.84 KK8 pKa = 6.92 RR9 pKa = 11.84 HH10 pKa = 5.04 KK11 pKa = 10.06 MATHH15 pKa = 6.02 KK16 pKa = 10.34 RR17 pKa = 11.84 KK18 pKa = 9.84 KK19 pKa = 9.28 RR20 pKa = 11.84 LRR22 pKa = 11.84 KK23 pKa = 9.16 NRR25 pKa = 11.84 HH26 pKa = 4.6 KK27 pKa = 10.96 KK28 pKa = 10.03 KK29 pKa = 10.26 IRR31 pKa = 3.43
Molecular weight: 3.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.357
IPC2_protein 10.789
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.398
Grimsley 12.544
Solomon 12.983
Lehninger 12.896
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.135
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.011
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3169
0
3169
1115965
22
2657
352.2
39.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.752 ± 0.04
0.708 ± 0.015
5.365 ± 0.033
6.652 ± 0.043
5.644 ± 0.039
6.218 ± 0.039
1.462 ± 0.018
8.884 ± 0.05
7.901 ± 0.043
9.47 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.902 ± 0.019
6.189 ± 0.04
3.642 ± 0.025
3.079 ± 0.024
3.718 ± 0.026
6.894 ± 0.04
5.214 ± 0.034
6.131 ± 0.033
1.04 ± 0.018
4.135 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here