BtRf-AlphaCoV/HuB2013

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Decacovirus; Rhinolophus ferrumequinum alphacoronavirus HuB-2013

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U1UZ30|A0A0U1UZ30_9ALPC 3C-like proteinase OS=BtRf-AlphaCoV/HuB2013 OX=1503292 PE=3 SV=1
MM1 pKa = 8.24DD2 pKa = 4.77IFNMSVLLFIVLCYY16 pKa = 10.24FEE18 pKa = 5.28VVLFALQAVFLGVEE32 pKa = 4.34LTSSVLFSSFILGCVAALFVTEE54 pKa = 5.74LPMQFLDD61 pKa = 4.87IIDD64 pKa = 5.34FYY66 pKa = 10.62TSRR69 pKa = 11.84LVHH72 pKa = 5.87TPVIRR77 pKa = 11.84TLAVSS82 pKa = 3.83

Molecular weight: 9.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U1WJW2|A0A0U1WJW2_9ALPC Spike glycoprotein OS=BtRf-AlphaCoV/HuB2013 OX=1503292 PE=4 SV=1
MM1 pKa = 7.6ASVSFQEE8 pKa = 4.47EE9 pKa = 3.91KK10 pKa = 10.28RR11 pKa = 11.84GRR13 pKa = 11.84SGKK16 pKa = 10.09VPLSYY21 pKa = 10.71YY22 pKa = 10.67NPVIVSGDD30 pKa = 3.33KK31 pKa = 10.81PFWKK35 pKa = 10.34VMPNNAVPKK44 pKa = 10.6GKK46 pKa = 10.41GNKK49 pKa = 8.87DD50 pKa = 3.28QQIGYY55 pKa = 8.97WNEE58 pKa = 3.5QPRR61 pKa = 11.84YY62 pKa = 9.28RR63 pKa = 11.84MVRR66 pKa = 11.84GTRR69 pKa = 11.84KK70 pKa = 9.91DD71 pKa = 4.11LPSKK75 pKa = 8.43WHH77 pKa = 6.58FYY79 pKa = 10.71YY80 pKa = 10.91LGTGPHH86 pKa = 6.66AEE88 pKa = 4.11SKK90 pKa = 10.53FRR92 pKa = 11.84TRR94 pKa = 11.84TEE96 pKa = 3.51GVYY99 pKa = 8.8WVAVQGSKK107 pKa = 9.39TEE109 pKa = 3.99PTGLGTRR116 pKa = 11.84KK117 pKa = 9.96RR118 pKa = 11.84NAEE121 pKa = 3.92LVNPEE126 pKa = 4.35FAIQLPAAIEE136 pKa = 4.03IQEE139 pKa = 4.22NTVSRR144 pKa = 11.84GNSRR148 pKa = 11.84SQSSNRR154 pKa = 11.84DD155 pKa = 2.93RR156 pKa = 11.84SQSGNRR162 pKa = 11.84SQNDD166 pKa = 3.31QKK168 pKa = 11.2QGNQNQKK175 pKa = 10.14SRR177 pKa = 11.84SNSQSRR183 pKa = 11.84KK184 pKa = 9.77GNQNSNDD191 pKa = 3.6SAVDD195 pKa = 2.96IVAAVKK201 pKa = 10.1QALKK205 pKa = 10.54EE206 pKa = 3.9LGVTNEE212 pKa = 4.09SNKK215 pKa = 9.98KK216 pKa = 9.75GKK218 pKa = 9.99NSGTNTPKK226 pKa = 10.12EE227 pKa = 4.09QRR229 pKa = 11.84SKK231 pKa = 11.3SPARR235 pKa = 11.84SPTVQKK241 pKa = 10.58KK242 pKa = 7.53QLEE245 pKa = 4.23RR246 pKa = 11.84PPWKK250 pKa = 9.82RR251 pKa = 11.84VPNSTEE257 pKa = 4.38DD258 pKa = 3.29VTKK261 pKa = 10.8CFGVRR266 pKa = 11.84DD267 pKa = 3.65THH269 pKa = 7.89RR270 pKa = 11.84NFGDD274 pKa = 3.47ADD276 pKa = 3.89LVRR279 pKa = 11.84NGIDD283 pKa = 3.11AKK285 pKa = 10.61HH286 pKa = 5.49YY287 pKa = 7.59PQLAEE292 pKa = 4.62LVPTPAAVLFGGEE305 pKa = 4.33VVTTEE310 pKa = 3.79VGSDD314 pKa = 3.34VEE316 pKa = 3.69ITYY319 pKa = 9.76IYY321 pKa = 10.16KK322 pKa = 9.86MKK324 pKa = 10.6VPKK327 pKa = 9.42TDD329 pKa = 3.04KK330 pKa = 10.77NLPAFLKK337 pKa = 10.43QVSAYY342 pKa = 9.25SQPSQAAEE350 pKa = 4.21VPSQLNPTAVAFQPLAEE367 pKa = 4.83DD368 pKa = 3.82EE369 pKa = 4.17QVEE372 pKa = 5.14IIDD375 pKa = 3.63QVYY378 pKa = 10.91GSFDD382 pKa = 3.02AA383 pKa = 5.67

Molecular weight: 42.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

9083

65

6727

1297.6

144.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.441 ± 0.254

3.138 ± 0.622

5.075 ± 0.593

4.393 ± 0.328

5.67 ± 0.362

6.43 ± 0.524

1.717 ± 0.23

5.097 ± 0.489

6.022 ± 0.881

8.378 ± 0.937

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.092 ± 0.242

6.22 ± 0.567

3.567 ± 0.358

3.182 ± 0.65

3.171 ± 0.41

7.321 ± 0.464

5.956 ± 0.509

10.173 ± 0.387

1.2 ± 0.294

4.756 ± 0.21
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski