Amino acid dipepetide frequency for Lactococcus phage 936 group phage PhiD.18

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.92AlaAla: 0.92 ± 0.437
0.115AlaCys: 0.115 ± 0.116
3.794AlaAsp: 3.794 ± 0.642
4.484AlaGlu: 4.484 ± 0.906
3.564AlaPhe: 3.564 ± 0.951
4.484AlaGly: 4.484 ± 0.879
0.805AlaHis: 0.805 ± 0.376
4.599AlaIle: 4.599 ± 0.84
6.553AlaLys: 6.553 ± 1.009
6.898AlaLeu: 6.898 ± 1.018
2.644AlaMet: 2.644 ± 0.624
4.599AlaAsn: 4.599 ± 0.87
0.805AlaPro: 0.805 ± 0.387
2.989AlaGln: 2.989 ± 0.642
2.299AlaArg: 2.299 ± 0.467
3.104AlaSer: 3.104 ± 0.72
3.104AlaThr: 3.104 ± 0.703
4.484AlaVal: 4.484 ± 1.156
2.299AlaTrp: 2.299 ± 0.877
1.61AlaTyr: 1.61 ± 0.361
0.0AlaXaa: 0.0 ± 0.0
Cys
0.23CysAla: 0.23 ± 0.17
0.0CysCys: 0.0 ± 0.0
0.23CysAsp: 0.23 ± 0.181
0.23CysGlu: 0.23 ± 0.148
0.115CysPhe: 0.115 ± 0.117
0.805CysGly: 0.805 ± 0.348
0.115CysHis: 0.115 ± 0.134
0.345CysIle: 0.345 ± 0.192
0.69CysLys: 0.69 ± 0.365
0.115CysLeu: 0.115 ± 0.128
0.23CysMet: 0.23 ± 0.181
0.575CysAsn: 0.575 ± 0.285
0.115CysPro: 0.115 ± 0.131
0.23CysGln: 0.23 ± 0.161
0.46CysArg: 0.46 ± 0.225
0.115CysSer: 0.115 ± 0.111
0.23CysThr: 0.23 ± 0.163
0.345CysVal: 0.345 ± 0.209
0.23CysTrp: 0.23 ± 0.2
0.345CysTyr: 0.345 ± 0.214
0.0CysXaa: 0.0 ± 0.0
Asp
2.299AspAla: 2.299 ± 0.626
0.115AspCys: 0.115 ± 0.118
3.219AspAsp: 3.219 ± 0.68
3.564AspGlu: 3.564 ± 0.691
3.679AspPhe: 3.679 ± 0.624
3.794AspGly: 3.794 ± 0.602
0.92AspHis: 0.92 ± 0.352
4.024AspIle: 4.024 ± 0.661
5.748AspLys: 5.748 ± 0.669
6.093AspLeu: 6.093 ± 0.821
1.15AspMet: 1.15 ± 0.271
3.564AspAsn: 3.564 ± 0.675
1.61AspPro: 1.61 ± 0.476
0.69AspGln: 0.69 ± 0.299
1.38AspArg: 1.38 ± 0.512
3.564AspSer: 3.564 ± 0.733
3.679AspThr: 3.679 ± 0.675
2.989AspVal: 2.989 ± 0.674
0.805AspTrp: 0.805 ± 0.257
2.759AspTyr: 2.759 ± 0.587
0.0AspXaa: 0.0 ± 0.0
Glu
4.369GluAla: 4.369 ± 0.643
0.575GluCys: 0.575 ± 0.319
3.334GluAsp: 3.334 ± 0.644
5.289GluGlu: 5.289 ± 1.144
3.679GluPhe: 3.679 ± 0.695
2.414GluGly: 2.414 ± 0.546
1.15GluHis: 1.15 ± 0.37
6.323GluIle: 6.323 ± 0.936
5.404GluLys: 5.404 ± 1.06
10.002GluLeu: 10.002 ± 1.475
2.184GluMet: 2.184 ± 0.458
4.599GluAsn: 4.599 ± 0.836
1.035GluPro: 1.035 ± 0.408
3.909GluGln: 3.909 ± 0.773
2.989GluArg: 2.989 ± 0.66
4.024GluSer: 4.024 ± 0.511
4.139GluThr: 4.139 ± 0.632
4.599GluVal: 4.599 ± 0.733
0.92GluTrp: 0.92 ± 0.272
3.104GluTyr: 3.104 ± 0.701
0.0GluXaa: 0.0 ± 0.0
Phe
2.644PheAla: 2.644 ± 0.519
0.23PheCys: 0.23 ± 0.152
2.989PheAsp: 2.989 ± 0.526
2.644PheGlu: 2.644 ± 0.593
1.725PhePhe: 1.725 ± 0.53
2.184PheGly: 2.184 ± 0.503
0.345PheHis: 0.345 ± 0.238
3.104PheIle: 3.104 ± 0.575
3.679PheLys: 3.679 ± 0.636
2.759PheLeu: 2.759 ± 0.54
0.805PheMet: 0.805 ± 0.282
2.759PheAsn: 2.759 ± 0.611
1.035PhePro: 1.035 ± 0.361
1.495PheGln: 1.495 ± 0.381
1.265PheArg: 1.265 ± 0.355
4.254PheSer: 4.254 ± 0.801
3.219PheThr: 3.219 ± 0.447
2.299PheVal: 2.299 ± 0.467
0.23PheTrp: 0.23 ± 0.142
1.495PheTyr: 1.495 ± 0.42
0.0PheXaa: 0.0 ± 0.0
Gly
4.024GlyAla: 4.024 ± 1.264
0.46GlyCys: 0.46 ± 0.206
3.219GlyAsp: 3.219 ± 0.722
4.139GlyGlu: 4.139 ± 0.673
2.529GlyPhe: 2.529 ± 0.563
4.484GlyGly: 4.484 ± 0.586
0.92GlyHis: 0.92 ± 0.321
4.369GlyIle: 4.369 ± 1.198
6.438GlyLys: 6.438 ± 0.853
5.978GlyLeu: 5.978 ± 1.085
1.38GlyMet: 1.38 ± 0.431
3.909GlyAsn: 3.909 ± 0.709
0.23GlyPro: 0.23 ± 0.158
2.414GlyGln: 2.414 ± 0.436
1.954GlyArg: 1.954 ± 0.406
4.944GlySer: 4.944 ± 0.874
3.909GlyThr: 3.909 ± 1.007
5.748GlyVal: 5.748 ± 1.117
1.15GlyTrp: 1.15 ± 0.341
2.989GlyTyr: 2.989 ± 0.618
0.0GlyXaa: 0.0 ± 0.0
His
0.805HisAla: 0.805 ± 0.239
0.46HisCys: 0.46 ± 0.249
0.69HisAsp: 0.69 ± 0.273
0.575HisGlu: 0.575 ± 0.214
0.345HisPhe: 0.345 ± 0.202
1.61HisGly: 1.61 ± 0.538
0.115HisHis: 0.115 ± 0.128
1.035HisIle: 1.035 ± 0.309
0.92HisLys: 0.92 ± 0.289
0.92HisLeu: 0.92 ± 0.33
0.115HisMet: 0.115 ± 0.129
1.38HisAsn: 1.38 ± 0.525
0.115HisPro: 0.115 ± 0.104
0.23HisGln: 0.23 ± 0.185
0.46HisArg: 0.46 ± 0.237
0.46HisSer: 0.46 ± 0.268
1.265HisThr: 1.265 ± 0.383
0.69HisVal: 0.69 ± 0.277
0.0HisTrp: 0.0 ± 0.0
0.575HisTyr: 0.575 ± 0.267
0.0HisXaa: 0.0 ± 0.0
Ile
5.633IleAla: 5.633 ± 0.92
0.0IleCys: 0.0 ± 0.0
3.794IleAsp: 3.794 ± 0.673
6.783IleGlu: 6.783 ± 0.81
3.104IlePhe: 3.104 ± 0.582
3.909IleGly: 3.909 ± 0.829
0.92IleHis: 0.92 ± 0.331
4.714IleIle: 4.714 ± 0.683
6.438IleLys: 6.438 ± 0.803
5.174IleLeu: 5.174 ± 0.864
1.84IleMet: 1.84 ± 0.42
4.944IleAsn: 4.944 ± 0.602
1.954IlePro: 1.954 ± 0.412
2.644IleGln: 2.644 ± 0.472
1.954IleArg: 1.954 ± 0.526
3.794IleSer: 3.794 ± 0.792
5.289IleThr: 5.289 ± 0.727
4.714IleVal: 4.714 ± 0.731
1.15IleTrp: 1.15 ± 0.385
2.529IleTyr: 2.529 ± 0.515
0.0IleXaa: 0.0 ± 0.0
Lys
6.553LysAla: 6.553 ± 1.05
0.46LysCys: 0.46 ± 0.229
4.484LysAsp: 4.484 ± 0.614
8.623LysGlu: 8.623 ± 1.41
2.069LysPhe: 2.069 ± 0.413
5.748LysGly: 5.748 ± 0.922
1.265LysHis: 1.265 ± 0.453
5.519LysIle: 5.519 ± 0.678
8.853LysLys: 8.853 ± 1.079
7.473LysLeu: 7.473 ± 0.731
3.449LysMet: 3.449 ± 0.468
5.404LysAsn: 5.404 ± 0.777
1.265LysPro: 1.265 ± 0.428
3.449LysGln: 3.449 ± 0.811
2.874LysArg: 2.874 ± 0.709
5.059LysSer: 5.059 ± 0.799
5.633LysThr: 5.633 ± 0.752
5.519LysVal: 5.519 ± 0.795
1.495LysTrp: 1.495 ± 0.339
3.449LysTyr: 3.449 ± 0.656
0.0LysXaa: 0.0 ± 0.0
Leu
5.059LeuAla: 5.059 ± 0.761
0.23LeuCys: 0.23 ± 0.162
4.944LeuAsp: 4.944 ± 0.664
5.633LeuGlu: 5.633 ± 0.848
3.334LeuPhe: 3.334 ± 0.638
4.944LeuGly: 4.944 ± 0.931
1.265LeuHis: 1.265 ± 0.389
7.588LeuIle: 7.588 ± 0.982
7.933LeuLys: 7.933 ± 0.804
6.668LeuLeu: 6.668 ± 1.064
1.495LeuMet: 1.495 ± 0.481
5.174LeuAsn: 5.174 ± 0.834
2.989LeuPro: 2.989 ± 0.624
3.104LeuGln: 3.104 ± 0.508
2.874LeuArg: 2.874 ± 0.624
4.829LeuSer: 4.829 ± 0.691
6.208LeuThr: 6.208 ± 0.713
6.208LeuVal: 6.208 ± 0.704
1.495LeuTrp: 1.495 ± 0.39
4.484LeuTyr: 4.484 ± 0.859
0.0LeuXaa: 0.0 ± 0.0
Met
2.069MetAla: 2.069 ± 0.573
0.115MetCys: 0.115 ± 0.117
1.265MetAsp: 1.265 ± 0.384
1.954MetGlu: 1.954 ± 0.59
0.46MetPhe: 0.46 ± 0.23
1.15MetGly: 1.15 ± 0.378
0.23MetHis: 0.23 ± 0.137
2.299MetIle: 2.299 ± 0.404
2.874MetLys: 2.874 ± 0.548
1.15MetLeu: 1.15 ± 0.405
0.46MetMet: 0.46 ± 0.229
2.069MetAsn: 2.069 ± 0.495
0.345MetPro: 0.345 ± 0.189
1.954MetGln: 1.954 ± 0.403
0.345MetArg: 0.345 ± 0.23
1.61MetSer: 1.61 ± 0.428
1.954MetThr: 1.954 ± 0.529
1.035MetVal: 1.035 ± 0.281
0.115MetTrp: 0.115 ± 0.111
1.15MetTyr: 1.15 ± 0.416
0.0MetXaa: 0.0 ± 0.0
Asn
5.633AsnAla: 5.633 ± 1.076
0.23AsnCys: 0.23 ± 0.174
4.484AsnAsp: 4.484 ± 0.825
4.944AsnGlu: 4.944 ± 0.982
1.84AsnPhe: 1.84 ± 0.525
6.093AsnGly: 6.093 ± 0.839
0.69AsnHis: 0.69 ± 0.338
4.254AsnIle: 4.254 ± 0.718
5.289AsnLys: 5.289 ± 0.974
5.863AsnLeu: 5.863 ± 0.814
1.265AsnMet: 1.265 ± 0.365
4.369AsnAsn: 4.369 ± 1.024
2.069AsnPro: 2.069 ± 0.573
1.84AsnGln: 1.84 ± 0.5
2.184AsnArg: 2.184 ± 0.44
4.599AsnSer: 4.599 ± 0.601
4.024AsnThr: 4.024 ± 0.698
3.334AsnVal: 3.334 ± 0.662
0.92AsnTrp: 0.92 ± 0.392
2.529AsnTyr: 2.529 ± 0.622
0.0AsnXaa: 0.0 ± 0.0
Pro
1.38ProAla: 1.38 ± 0.376
0.115ProCys: 0.115 ± 0.127
1.61ProAsp: 1.61 ± 0.497
1.84ProGlu: 1.84 ± 0.523
0.92ProPhe: 0.92 ± 0.297
0.23ProGly: 0.23 ± 0.177
0.0ProHis: 0.0 ± 0.0
1.38ProIle: 1.38 ± 0.404
1.725ProLys: 1.725 ± 0.444
1.84ProLeu: 1.84 ± 0.364
0.575ProMet: 0.575 ± 0.23
2.414ProAsn: 2.414 ± 0.838
0.575ProPro: 0.575 ± 0.283
0.575ProGln: 0.575 ± 0.29
0.46ProArg: 0.46 ± 0.219
0.92ProSer: 0.92 ± 0.344
2.414ProThr: 2.414 ± 0.569
1.84ProVal: 1.84 ± 0.396
0.115ProTrp: 0.115 ± 0.127
0.92ProTyr: 0.92 ± 0.346
0.0ProXaa: 0.0 ± 0.0
Gln
3.679GlnAla: 3.679 ± 0.813
0.23GlnCys: 0.23 ± 0.167
1.954GlnAsp: 1.954 ± 0.508
2.759GlnGlu: 2.759 ± 0.578
1.265GlnPhe: 1.265 ± 0.466
2.759GlnGly: 2.759 ± 0.538
0.345GlnHis: 0.345 ± 0.173
1.38GlnIle: 1.38 ± 0.309
2.989GlnLys: 2.989 ± 0.625
2.759GlnLeu: 2.759 ± 0.503
1.035GlnMet: 1.035 ± 0.316
1.725GlnAsn: 1.725 ± 0.375
1.15GlnPro: 1.15 ± 0.315
1.61GlnGln: 1.61 ± 0.492
1.954GlnArg: 1.954 ± 0.506
2.759GlnSer: 2.759 ± 0.507
2.874GlnThr: 2.874 ± 0.654
2.414GlnVal: 2.414 ± 0.601
0.69GlnTrp: 0.69 ± 0.221
1.38GlnTyr: 1.38 ± 0.395
0.0GlnXaa: 0.0 ± 0.0
Arg
2.069ArgAla: 2.069 ± 0.497
0.23ArgCys: 0.23 ± 0.179
1.954ArgAsp: 1.954 ± 0.523
1.84ArgGlu: 1.84 ± 0.4
0.69ArgPhe: 0.69 ± 0.265
2.184ArgGly: 2.184 ± 0.392
0.805ArgHis: 0.805 ± 0.319
2.184ArgIle: 2.184 ± 0.461
3.679ArgLys: 3.679 ± 0.845
3.564ArgLeu: 3.564 ± 0.653
0.805ArgMet: 0.805 ± 0.361
2.414ArgAsn: 2.414 ± 0.505
0.575ArgPro: 0.575 ± 0.243
1.61ArgGln: 1.61 ± 0.418
2.184ArgArg: 2.184 ± 0.534
1.38ArgSer: 1.38 ± 0.413
1.84ArgThr: 1.84 ± 0.421
1.954ArgVal: 1.954 ± 0.517
0.345ArgTrp: 0.345 ± 0.225
1.954ArgTyr: 1.954 ± 0.497
0.0ArgXaa: 0.0 ± 0.0
Ser
4.829SerAla: 4.829 ± 1.219
0.805SerCys: 0.805 ± 0.384
3.104SerAsp: 3.104 ± 0.549
4.024SerGlu: 4.024 ± 0.704
2.644SerPhe: 2.644 ± 0.528
6.093SerGly: 6.093 ± 1.541
0.575SerHis: 0.575 ± 0.267
5.174SerIle: 5.174 ± 0.873
4.254SerLys: 4.254 ± 0.702
6.093SerLeu: 6.093 ± 1.06
1.38SerMet: 1.38 ± 0.329
3.794SerAsn: 3.794 ± 0.638
1.61SerPro: 1.61 ± 0.442
1.61SerGln: 1.61 ± 0.387
2.414SerArg: 2.414 ± 0.47
5.748SerSer: 5.748 ± 1.309
2.759SerThr: 2.759 ± 0.663
4.139SerVal: 4.139 ± 0.816
1.265SerTrp: 1.265 ± 0.348
1.84SerTyr: 1.84 ± 0.489
0.0SerXaa: 0.0 ± 0.0
Thr
5.863ThrAla: 5.863 ± 0.952
0.23ThrCys: 0.23 ± 0.187
3.219ThrAsp: 3.219 ± 0.698
5.404ThrGlu: 5.404 ± 0.751
2.989ThrPhe: 2.989 ± 0.542
4.829ThrGly: 4.829 ± 0.817
0.115ThrHis: 0.115 ± 0.117
4.254ThrIle: 4.254 ± 0.813
5.289ThrLys: 5.289 ± 0.644
5.059ThrLeu: 5.059 ± 0.841
1.035ThrMet: 1.035 ± 0.309
4.484ThrAsn: 4.484 ± 0.715
1.725ThrPro: 1.725 ± 0.341
3.219ThrGln: 3.219 ± 0.543
1.725ThrArg: 1.725 ± 0.468
4.139ThrSer: 4.139 ± 0.577
4.484ThrThr: 4.484 ± 0.547
4.714ThrVal: 4.714 ± 0.844
1.38ThrTrp: 1.38 ± 0.377
1.725ThrTyr: 1.725 ± 0.47
0.0ThrXaa: 0.0 ± 0.0
Val
3.679ValAla: 3.679 ± 0.567
0.575ValCys: 0.575 ± 0.319
4.254ValAsp: 4.254 ± 0.834
4.484ValGlu: 4.484 ± 0.711
2.644ValPhe: 2.644 ± 0.481
3.449ValGly: 3.449 ± 0.523
0.575ValHis: 0.575 ± 0.213
5.289ValIle: 5.289 ± 0.569
6.208ValLys: 6.208 ± 0.785
3.449ValLeu: 3.449 ± 0.514
1.725ValMet: 1.725 ± 0.503
3.219ValAsn: 3.219 ± 0.733
1.61ValPro: 1.61 ± 0.498
1.954ValGln: 1.954 ± 0.582
2.874ValArg: 2.874 ± 0.766
6.208ValSer: 6.208 ± 1.526
5.174ValThr: 5.174 ± 1.047
3.219ValVal: 3.219 ± 0.681
0.23ValTrp: 0.23 ± 0.167
2.874ValTyr: 2.874 ± 0.61
0.0ValXaa: 0.0 ± 0.0
Trp
0.805TrpAla: 0.805 ± 0.298
0.23TrpCys: 0.23 ± 0.157
1.035TrpAsp: 1.035 ± 0.44
0.805TrpGlu: 0.805 ± 0.315
1.265TrpPhe: 1.265 ± 0.666
0.92TrpGly: 0.92 ± 0.348
0.23TrpHis: 0.23 ± 0.19
0.575TrpIle: 0.575 ± 0.252
1.15TrpLys: 1.15 ± 0.305
1.15TrpLeu: 1.15 ± 0.417
0.345TrpMet: 0.345 ± 0.185
1.725TrpAsn: 1.725 ± 0.459
0.0TrpPro: 0.0 ± 0.0
0.92TrpGln: 0.92 ± 0.304
0.345TrpArg: 0.345 ± 0.249
1.15TrpSer: 1.15 ± 0.345
0.92TrpThr: 0.92 ± 0.359
0.575TrpVal: 0.575 ± 0.245
0.23TrpTrp: 0.23 ± 0.159
1.035TrpTyr: 1.035 ± 0.385
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.61TyrAla: 1.61 ± 0.52
0.345TyrCys: 0.345 ± 0.297
2.069TyrAsp: 2.069 ± 0.707
4.024TyrGlu: 4.024 ± 0.809
2.299TyrPhe: 2.299 ± 0.469
3.104TyrGly: 3.104 ± 0.621
1.38TyrHis: 1.38 ± 0.41
2.989TyrIle: 2.989 ± 0.786
2.529TyrLys: 2.529 ± 0.62
3.449TyrLeu: 3.449 ± 0.773
0.575TyrMet: 0.575 ± 0.289
3.334TyrAsn: 3.334 ± 0.656
1.035TyrPro: 1.035 ± 0.39
1.265TyrGln: 1.265 ± 0.605
1.265TyrArg: 1.265 ± 0.367
1.495TyrSer: 1.495 ± 0.397
2.759TyrThr: 2.759 ± 0.632
2.874TyrVal: 2.874 ± 0.481
0.345TyrTrp: 0.345 ± 0.211
2.184TyrTyr: 2.184 ± 0.602
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (8699 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski