Actinidia chinensis var. chinensis (Chinese soft-hair kiwi)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; Ericales; Actinidiaceae; Actinidia; Actinidia chinensis

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32913 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R6PPN1|A0A2R6PPN1_ACTCC ZZ-type zinc finger-containing protein OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc25744 PE=4 SV=1
MM1 pKa = 7.75GDD3 pKa = 3.51SEE5 pKa = 4.84TVVAQTSAVMGYY17 pKa = 8.2TSAGYY22 pKa = 10.69SSTNYY27 pKa = 10.48SSISSNVASDD37 pKa = 3.57AGAFADD43 pKa = 3.7ATTTRR48 pKa = 11.84DD49 pKa = 3.05LVAAVAVADD58 pKa = 4.0VANTVADD65 pKa = 4.2GSDD68 pKa = 3.39VGGEE72 pKa = 4.11AYY74 pKa = 8.93STYY77 pKa = 10.83SNSAIQGAPATMLYY91 pKa = 10.79GSDD94 pKa = 3.79GVDD97 pKa = 2.94AMEE100 pKa = 5.6NIAASTKK107 pKa = 10.04ISTGSSQVTGADD119 pKa = 3.08SSVNGNDD126 pKa = 3.14VIEE129 pKa = 4.59ARR131 pKa = 11.84DD132 pKa = 3.49IVLTGISKK140 pKa = 10.6NGNSSDD146 pKa = 3.59KK147 pKa = 10.44VHH149 pKa = 6.66EE150 pKa = 4.58SSSDD154 pKa = 3.32HH155 pKa = 5.94QLVNGSS161 pKa = 3.39

Molecular weight: 15.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R6R1C9|A0A2R6R1C9_ACTCC DNA mismatch repair protein like OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc10995 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.39LPYY5 pKa = 9.61EE6 pKa = 4.23PQHH9 pKa = 6.49RR10 pKa = 11.84GFMTQIYY17 pKa = 9.59DD18 pKa = 3.72LGSRR22 pKa = 11.84TQAPNTLTLAPTSPSLVVHH41 pKa = 6.44RR42 pKa = 11.84HH43 pKa = 4.4HH44 pKa = 6.85HH45 pKa = 5.21HH46 pKa = 6.38TPTVINSNTNIQPPLEE62 pKa = 4.18VATPKK67 pKa = 10.4TSMVVGLLRR76 pKa = 11.84WGLGFNGGMVMRR88 pKa = 11.84FAIPVAVVQFMTAATRR104 pKa = 11.84IKK106 pKa = 10.19RR107 pKa = 11.84QQLDD111 pKa = 3.53SRR113 pKa = 11.84WRR115 pKa = 11.84RR116 pKa = 11.84RR117 pKa = 11.84RR118 pKa = 11.84LNSNGNYY125 pKa = 9.05SRR127 pKa = 11.84HH128 pKa = 5.67PVRR131 pKa = 11.84ICAFCEE137 pKa = 4.24CEE139 pKa = 3.92FQEE142 pKa = 5.94DD143 pKa = 3.54IGDD146 pKa = 4.19EE147 pKa = 3.68IAAIKK152 pKa = 9.33VRR154 pKa = 11.84KK155 pKa = 9.92SGVCRR160 pKa = 11.84RR161 pKa = 11.84IFLLCTPP168 pKa = 5.05

Molecular weight: 19.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

32853

60

32913

14039321

13

5453

426.6

47.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.781 ± 0.012

1.841 ± 0.007

5.304 ± 0.008

6.526 ± 0.014

4.143 ± 0.008

6.742 ± 0.013

2.389 ± 0.006

5.171 ± 0.008

5.969 ± 0.011

9.586 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.423 ± 0.005

4.383 ± 0.008

5.098 ± 0.014

3.674 ± 0.009

5.459 ± 0.01

9.017 ± 0.014

4.891 ± 0.009

6.592 ± 0.009

1.276 ± 0.004

2.736 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski