Xylella phage Xfas53
Average proteome isoelectric point is 7.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C8CLG8|C8CLG8_9CAUD Gp15 OS=Xylella phage Xfas53 OX=670252 GN=Xfas53_gene15 PE=4 SV=1
MM1 pKa = 7.33 TGIGYY6 pKa = 10.41 SNYY9 pKa = 9.75 NDD11 pKa = 4.13 PRR13 pKa = 11.84 LQPPQDD19 pKa = 3.45 DD20 pKa = 3.7 AKK22 pKa = 10.74 EE23 pKa = 4.07 YY24 pKa = 10.08 FAEE27 pKa = 4.14 RR28 pKa = 11.84 VNARR32 pKa = 11.84 VQDD35 pKa = 3.99 YY36 pKa = 11.04 LSDD39 pKa = 3.73 PEE41 pKa = 5.24 KK42 pKa = 10.65 IEE44 pKa = 4.85 EE45 pKa = 4.01 ADD47 pKa = 3.36 EE48 pKa = 4.3 WVDD51 pKa = 3.6 GTLSEE56 pKa = 4.05 AHH58 pKa = 5.92 YY59 pKa = 11.08 KK60 pKa = 10.18 EE61 pKa = 4.16 MEE63 pKa = 4.01 IVLADD68 pKa = 3.75 LHH70 pKa = 6.86 ALPSDD75 pKa = 3.55 QLSDD79 pKa = 3.47 SDD81 pKa = 4.02 VLARR85 pKa = 11.84 LYY87 pKa = 11.18 ALAEE91 pKa = 4.22 VQGLARR97 pKa = 11.84 MEE99 pKa = 4.01 QLRR102 pKa = 11.84 ILAEE106 pKa = 3.83 QDD108 pKa = 3.19 VKK110 pKa = 11.54 EE111 pKa = 4.36 EE112 pKa = 3.87 MRR114 pKa = 11.84 HH115 pKa = 4.83 EE116 pKa = 4.37 SEE118 pKa = 4.94 CFHH121 pKa = 6.16 MMWGDD126 pKa = 3.55 VMQEE130 pKa = 3.85 EE131 pKa = 5.18 YY132 pKa = 11.36 AA133 pKa = 3.8
Molecular weight: 15.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.229
IPC2_protein 4.215
IPC_protein 4.151
Toseland 3.986
ProMoST 4.266
Dawson 4.113
Bjellqvist 4.253
Wikipedia 3.986
Rodwell 3.986
Grimsley 3.897
Solomon 4.101
Lehninger 4.062
Nozaki 4.215
DTASelect 4.368
Thurlkill 3.999
EMBOSS 3.999
Sillero 4.266
Patrickios 3.35
IPC_peptide 4.101
IPC2_peptide 4.253
IPC2.peptide.svr19 4.171
Protein with the highest isoelectric point:
>tr|C8CLG0|C8CLG0_9CAUD DNA polymerase A OS=Xylella phage Xfas53 OX=670252 GN=pol PE=3 SV=1
MM1 pKa = 7.59 TIPRR5 pKa = 11.84 EE6 pKa = 4.07 RR7 pKa = 11.84 TIEE10 pKa = 4.14 RR11 pKa = 11.84 YY12 pKa = 9.51 LVAQVRR18 pKa = 11.84 AKK20 pKa = 10.4 GGEE23 pKa = 3.67 IRR25 pKa = 11.84 KK26 pKa = 8.85 VKK28 pKa = 9.34 WGGRR32 pKa = 11.84 HH33 pKa = 5.33 GAPDD37 pKa = 4.98 RR38 pKa = 11.84 IAMLPNGRR46 pKa = 11.84 TLWVEE51 pKa = 4.21 LKK53 pKa = 10.98 APGQQCTPHH62 pKa = 5.68 QVRR65 pKa = 11.84 EE66 pKa = 4.1 HH67 pKa = 4.96 EE68 pKa = 4.44 RR69 pKa = 11.84 MRR71 pKa = 11.84 GMGQRR76 pKa = 11.84 VVVVDD81 pKa = 3.48 SLKK84 pKa = 11.21 GVDD87 pKa = 3.64 EE88 pKa = 4.38 VLAA91 pKa = 4.62
Molecular weight: 10.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.329
IPC2_protein 9.487
IPC_protein 10.175
Toseland 10.774
ProMoST 10.452
Dawson 10.833
Bjellqvist 10.526
Wikipedia 11.038
Rodwell 11.008
Grimsley 10.862
Solomon 10.994
Lehninger 10.965
Nozaki 10.76
DTASelect 10.526
Thurlkill 10.76
EMBOSS 11.184
Sillero 10.774
Patrickios 10.789
IPC_peptide 11.008
IPC2_peptide 9.458
IPC2.peptide.svr19 8.751
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
45
0
45
11483
54
845
255.2
27.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.148 ± 0.504
1.245 ± 0.172
5.391 ± 0.218
5.382 ± 0.356
3.074 ± 0.228
7.655 ± 0.355
2.552 ± 0.202
4.049 ± 0.185
4.084 ± 0.32
8.552 ± 0.369
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.282 ± 0.166
3.17 ± 0.225
5.661 ± 0.245
4.572 ± 0.345
6.941 ± 0.375
5.704 ± 0.545
6.261 ± 0.36
6.801 ± 0.301
1.655 ± 0.129
2.822 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here