Sphingomonas sp. (strain SKA58)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3892 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1N8V0|Q1N8V0_SPHSS Uncharacterized protein OS=Sphingomonas sp. (strain SKA58) OX=314266 GN=SKA58_19670 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84STYY5 pKa = 10.97DD6 pKa = 3.08GATVRR11 pKa = 11.84LYY13 pKa = 10.95HH14 pKa = 6.71LGDD17 pKa = 4.57DD18 pKa = 4.34GGVTTLLYY26 pKa = 11.0GPLTEE31 pKa = 4.53ALRR34 pKa = 11.84VADD37 pKa = 3.95QQPADD42 pKa = 3.79MQDD45 pKa = 3.25GLFIATDD52 pKa = 3.65NDD54 pKa = 3.33VVAYY58 pKa = 10.5LDD60 pKa = 5.12LIAEE64 pKa = 4.5

Molecular weight: 6.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q1NCD5|Q1NCD5_SPHSS Uncharacterized protein OS=Sphingomonas sp. (strain SKA58) OX=314266 GN=SKA58_12712 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.11EE3 pKa = 3.57SAALDD8 pKa = 3.32EE9 pKa = 4.55RR10 pKa = 11.84LRR12 pKa = 11.84EE13 pKa = 4.04EE14 pKa = 4.36TLFLAVTRR22 pKa = 11.84PTMWLGVPLEE32 pKa = 4.22ASLPIALAACLTLIVSGNPLYY53 pKa = 11.0AGAIGGACLAVARR66 pKa = 11.84LIVRR70 pKa = 11.84HH71 pKa = 6.34DD72 pKa = 3.7ANAFRR77 pKa = 11.84LLWLWTMTKK86 pKa = 10.01ARR88 pKa = 11.84SRR90 pKa = 11.84NRR92 pKa = 11.84VWWGGSSYY100 pKa = 11.48SPLPVAGLKK109 pKa = 10.45RR110 pKa = 11.84KK111 pKa = 9.78GFARR115 pKa = 11.84GG116 pKa = 3.13

Molecular weight: 12.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3892

0

3892

1183197

20

1659

304.0

32.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.175 ± 0.058

0.797 ± 0.013

6.308 ± 0.033

5.1 ± 0.037

3.45 ± 0.025

8.773 ± 0.037

2.075 ± 0.021

5.153 ± 0.027

3.006 ± 0.031

9.932 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.674 ± 0.02

2.508 ± 0.024

5.301 ± 0.029

3.405 ± 0.026

7.385 ± 0.04

5.248 ± 0.027

5.109 ± 0.029

6.938 ± 0.031

1.434 ± 0.017

2.228 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski