Amino acid dipepetide frequency for Firmicutes bacterium ASF500

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.389AlaAla: 11.389 ± 0.15
1.363AlaCys: 1.363 ± 0.039
4.9AlaAsp: 4.9 ± 0.076
6.402AlaGlu: 6.402 ± 0.093
3.167AlaPhe: 3.167 ± 0.065
8.008AlaGly: 8.008 ± 0.11
1.462AlaHis: 1.462 ± 0.037
4.593AlaIle: 4.593 ± 0.073
4.185AlaLys: 4.185 ± 0.078
10.425AlaLeu: 10.425 ± 0.142
2.526AlaMet: 2.526 ± 0.052
2.363AlaAsn: 2.363 ± 0.061
3.932AlaPro: 3.932 ± 0.09
4.011AlaGln: 4.011 ± 0.066
5.011AlaArg: 5.011 ± 0.081
4.636AlaSer: 4.636 ± 0.079
3.358AlaThr: 3.358 ± 0.09
7.793AlaVal: 7.793 ± 0.109
0.944AlaTrp: 0.944 ± 0.032
2.669AlaTyr: 2.669 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
1.534CysAla: 1.534 ± 0.043
0.348CysCys: 0.348 ± 0.019
0.855CysAsp: 0.855 ± 0.024
0.785CysGlu: 0.785 ± 0.027
0.619CysPhe: 0.619 ± 0.025
1.781CysGly: 1.781 ± 0.05
0.315CysHis: 0.315 ± 0.016
0.693CysIle: 0.693 ± 0.027
0.658CysLys: 0.658 ± 0.028
1.386CysLeu: 1.386 ± 0.042
0.354CysMet: 0.354 ± 0.018
0.423CysAsn: 0.423 ± 0.023
0.865CysPro: 0.865 ± 0.038
0.573CysGln: 0.573 ± 0.026
0.981CysArg: 0.981 ± 0.037
0.844CysSer: 0.844 ± 0.027
0.805CysThr: 0.805 ± 0.03
1.2CysVal: 1.2 ± 0.037
0.193CysTrp: 0.193 ± 0.014
0.521CysTyr: 0.521 ± 0.031
0.0CysXaa: 0.0 ± 0.0
Asp
4.61AspAla: 4.61 ± 0.071
0.969AspCys: 0.969 ± 0.032
2.598AspAsp: 2.598 ± 0.06
3.708AspGlu: 3.708 ± 0.07
2.491AspPhe: 2.491 ± 0.057
5.244AspGly: 5.244 ± 0.106
1.015AspHis: 1.015 ± 0.037
3.323AspIle: 3.323 ± 0.075
2.736AspLys: 2.736 ± 0.055
5.113AspLeu: 5.113 ± 0.075
1.561AspMet: 1.561 ± 0.039
1.747AspAsn: 1.747 ± 0.055
2.705AspPro: 2.705 ± 0.058
1.799AspGln: 1.799 ± 0.049
3.532AspArg: 3.532 ± 0.072
3.07AspSer: 3.07 ± 0.062
2.99AspThr: 2.99 ± 0.057
3.405AspVal: 3.405 ± 0.063
0.78AspTrp: 0.78 ± 0.032
2.254AspTyr: 2.254 ± 0.053
0.0AspXaa: 0.0 ± 0.0
Glu
6.453GluAla: 6.453 ± 0.083
0.773GluCys: 0.773 ± 0.03
3.94GluAsp: 3.94 ± 0.064
6.181GluGlu: 6.181 ± 0.097
2.053GluPhe: 2.053 ± 0.051
5.068GluGly: 5.068 ± 0.074
1.384GluHis: 1.384 ± 0.041
4.005GluIle: 4.005 ± 0.075
4.318GluLys: 4.318 ± 0.077
7.503GluLeu: 7.503 ± 0.097
1.916GluMet: 1.916 ± 0.048
2.829GluAsn: 2.829 ± 0.053
2.348GluPro: 2.348 ± 0.063
3.051GluGln: 3.051 ± 0.069
4.788GluArg: 4.788 ± 0.096
2.672GluSer: 2.672 ± 0.049
3.394GluThr: 3.394 ± 0.071
4.266GluVal: 4.266 ± 0.066
0.714GluTrp: 0.714 ± 0.024
2.43GluTyr: 2.43 ± 0.054
0.0GluXaa: 0.0 ± 0.0
Phe
3.254PheAla: 3.254 ± 0.063
0.737PheCys: 0.737 ± 0.03
2.381PheAsp: 2.381 ± 0.041
2.114PheGlu: 2.114 ± 0.047
1.705PhePhe: 1.705 ± 0.042
2.817PheGly: 2.817 ± 0.062
0.784PheHis: 0.784 ± 0.03
1.885PheIle: 1.885 ± 0.044
1.452PheLys: 1.452 ± 0.042
3.797PheLeu: 3.797 ± 0.076
0.813PheMet: 0.813 ± 0.025
1.243PheAsn: 1.243 ± 0.038
1.556PhePro: 1.556 ± 0.039
1.722PheGln: 1.722 ± 0.043
1.981PheArg: 1.981 ± 0.048
2.693PheSer: 2.693 ± 0.055
2.401PheThr: 2.401 ± 0.054
2.381PheVal: 2.381 ± 0.059
0.501PheTrp: 0.501 ± 0.023
1.447PheTyr: 1.447 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
6.527GlyAla: 6.527 ± 0.091
1.398GlyCys: 1.398 ± 0.047
4.117GlyAsp: 4.117 ± 0.071
5.421GlyGlu: 5.421 ± 0.075
2.991GlyPhe: 2.991 ± 0.055
6.855GlyGly: 6.855 ± 0.126
1.467GlyHis: 1.467 ± 0.041
4.282GlyIle: 4.282 ± 0.076
4.322GlyLys: 4.322 ± 0.062
7.544GlyLeu: 7.544 ± 0.102
2.446GlyMet: 2.446 ± 0.058
2.524GlyAsn: 2.524 ± 0.076
2.369GlyPro: 2.369 ± 0.057
2.949GlyGln: 2.949 ± 0.06
4.759GlyArg: 4.759 ± 0.084
4.458GlySer: 4.458 ± 0.089
4.63GlyThr: 4.63 ± 0.155
5.813GlyVal: 5.813 ± 0.075
1.078GlyTrp: 1.078 ± 0.03
3.169GlyTyr: 3.169 ± 0.063
0.0GlyXaa: 0.0 ± 0.0
His
1.212HisAla: 1.212 ± 0.034
0.333HisCys: 0.333 ± 0.016
0.894HisAsp: 0.894 ± 0.033
0.987HisGlu: 0.987 ± 0.034
0.792HisPhe: 0.792 ± 0.028
1.378HisGly: 1.378 ± 0.039
0.402HisHis: 0.402 ± 0.03
1.305HisIle: 1.305 ± 0.045
0.651HisLys: 0.651 ± 0.026
1.683HisLeu: 1.683 ± 0.047
0.495HisMet: 0.495 ± 0.019
0.572HisAsn: 0.572 ± 0.023
1.015HisPro: 1.015 ± 0.034
0.625HisGln: 0.625 ± 0.028
1.049HisArg: 1.049 ± 0.034
1.007HisSer: 1.007 ± 0.031
1.081HisThr: 1.081 ± 0.039
1.055HisVal: 1.055 ± 0.035
0.242HisTrp: 0.242 ± 0.014
0.721HisTyr: 0.721 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
4.681IleAla: 4.681 ± 0.076
1.016IleCys: 1.016 ± 0.035
3.123IleAsp: 3.123 ± 0.065
3.113IleGlu: 3.113 ± 0.056
2.052IlePhe: 2.052 ± 0.049
3.666IleGly: 3.666 ± 0.071
1.02IleHis: 1.02 ± 0.032
2.92IleIle: 2.92 ± 0.072
2.294IleLys: 2.294 ± 0.058
5.307IleLeu: 5.307 ± 0.083
1.28IleMet: 1.28 ± 0.043
1.859IleAsn: 1.859 ± 0.05
2.903IlePro: 2.903 ± 0.059
2.185IleGln: 2.185 ± 0.046
3.197IleArg: 3.197 ± 0.059
3.551IleSer: 3.551 ± 0.065
3.404IleThr: 3.404 ± 0.085
3.594IleVal: 3.594 ± 0.058
0.586IleTrp: 0.586 ± 0.026
1.738IleTyr: 1.738 ± 0.043
0.0IleXaa: 0.0 ± 0.0
Lys
4.387LysAla: 4.387 ± 0.074
0.508LysCys: 0.508 ± 0.024
2.608LysAsp: 2.608 ± 0.057
3.594LysGlu: 3.594 ± 0.061
1.331LysPhe: 1.331 ± 0.036
3.521LysGly: 3.521 ± 0.056
0.776LysHis: 0.776 ± 0.028
2.813LysIle: 2.813 ± 0.059
3.6LysLys: 3.6 ± 0.078
4.592LysLeu: 4.592 ± 0.078
1.343LysMet: 1.343 ± 0.039
1.981LysAsn: 1.981 ± 0.049
2.061LysPro: 2.061 ± 0.053
1.799LysGln: 1.799 ± 0.045
2.991LysArg: 2.991 ± 0.064
2.498LysSer: 2.498 ± 0.058
2.841LysThr: 2.841 ± 0.062
3.061LysVal: 3.061 ± 0.061
0.486LysTrp: 0.486 ± 0.02
1.751LysTyr: 1.751 ± 0.042
0.0LysXaa: 0.0 ± 0.0
Leu
9.692LeuAla: 9.692 ± 0.149
1.91LeuCys: 1.91 ± 0.044
5.864LeuAsp: 5.864 ± 0.091
7.224LeuGlu: 7.224 ± 0.099
3.89LeuPhe: 3.89 ± 0.075
6.766LeuGly: 6.766 ± 0.096
1.573LeuHis: 1.573 ± 0.041
4.67LeuIle: 4.67 ± 0.088
4.426LeuLys: 4.426 ± 0.072
10.147LeuLeu: 10.147 ± 0.156
2.658LeuMet: 2.658 ± 0.058
3.317LeuAsn: 3.317 ± 0.062
4.782LeuPro: 4.782 ± 0.082
2.836LeuGln: 2.836 ± 0.05
6.099LeuArg: 6.099 ± 0.099
7.157LeuSer: 7.157 ± 0.099
6.253LeuThr: 6.253 ± 0.104
5.891LeuVal: 5.891 ± 0.09
1.121LeuTrp: 1.121 ± 0.037
3.296LeuTyr: 3.296 ± 0.064
0.0LeuXaa: 0.0 ± 0.0
Met
2.623MetAla: 2.623 ± 0.055
0.294MetCys: 0.294 ± 0.017
1.809MetAsp: 1.809 ± 0.045
2.28MetGlu: 2.28 ± 0.051
0.848MetPhe: 0.848 ± 0.031
2.15MetGly: 2.15 ± 0.047
0.267MetHis: 0.267 ± 0.017
1.288MetIle: 1.288 ± 0.041
1.76MetLys: 1.76 ± 0.042
2.492MetLeu: 2.492 ± 0.055
0.73MetMet: 0.73 ± 0.03
1.129MetAsn: 1.129 ± 0.035
1.133MetPro: 1.133 ± 0.033
0.679MetGln: 0.679 ± 0.026
1.387MetArg: 1.387 ± 0.04
1.734MetSer: 1.734 ± 0.044
1.619MetThr: 1.619 ± 0.038
1.843MetVal: 1.843 ± 0.044
0.207MetTrp: 0.207 ± 0.015
0.675MetTyr: 0.675 ± 0.025
0.0MetXaa: 0.0 ± 0.0
Asn
2.851AsnAla: 2.851 ± 0.056
0.473AsnCys: 0.473 ± 0.023
1.519AsnAsp: 1.519 ± 0.046
1.8AsnGlu: 1.8 ± 0.046
1.2AsnPhe: 1.2 ± 0.032
3.082AsnGly: 3.082 ± 0.085
0.629AsnHis: 0.629 ± 0.027
2.173AsnIle: 2.173 ± 0.048
1.352AsnLys: 1.352 ± 0.037
3.059AsnLeu: 3.059 ± 0.059
0.89AsnMet: 0.89 ± 0.031
1.114AsnAsn: 1.114 ± 0.041
1.787AsnPro: 1.787 ± 0.041
1.213AsnGln: 1.213 ± 0.037
2.094AsnArg: 2.094 ± 0.05
1.747AsnSer: 1.747 ± 0.043
2.019AsnThr: 2.019 ± 0.045
2.27AsnVal: 2.27 ± 0.053
0.384AsnTrp: 0.384 ± 0.017
1.254AsnTyr: 1.254 ± 0.049
0.0AsnXaa: 0.0 ± 0.0
Pro
4.652ProAla: 4.652 ± 0.095
0.61ProCys: 0.61 ± 0.03
3.033ProAsp: 3.033 ± 0.054
4.262ProGlu: 4.262 ± 0.079
1.555ProPhe: 1.555 ± 0.041
3.543ProGly: 3.543 ± 0.074
0.734ProHis: 0.734 ± 0.028
2.019ProIle: 2.019 ± 0.044
1.932ProLys: 1.932 ± 0.048
3.734ProLeu: 3.734 ± 0.069
1.043ProMet: 1.043 ± 0.035
1.302ProAsn: 1.302 ± 0.037
1.974ProPro: 1.974 ± 0.061
1.705ProGln: 1.705 ± 0.042
1.968ProArg: 1.968 ± 0.055
2.194ProSer: 2.194 ± 0.05
2.011ProThr: 2.011 ± 0.058
3.49ProVal: 3.49 ± 0.062
0.543ProTrp: 0.543 ± 0.024
1.478ProTyr: 1.478 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
3.833GlnAla: 3.833 ± 0.067
0.462GlnCys: 0.462 ± 0.025
1.875GlnAsp: 1.875 ± 0.042
2.96GlnGlu: 2.96 ± 0.056
1.324GlnPhe: 1.324 ± 0.034
2.688GlnGly: 2.688 ± 0.052
0.54GlnHis: 0.54 ± 0.021
2.033GlnIle: 2.033 ± 0.05
2.053GlnLys: 2.053 ± 0.046
3.542GlnLeu: 3.542 ± 0.065
1.149GlnMet: 1.149 ± 0.032
1.312GlnAsn: 1.312 ± 0.043
1.447GlnPro: 1.447 ± 0.038
1.283GlnGln: 1.283 ± 0.043
2.376GlnArg: 2.376 ± 0.055
2.128GlnSer: 2.128 ± 0.051
1.983GlnThr: 1.983 ± 0.053
2.648GlnVal: 2.648 ± 0.045
0.47GlnTrp: 0.47 ± 0.024
1.293GlnTyr: 1.293 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
4.973ArgAla: 4.973 ± 0.084
0.923ArgCys: 0.923 ± 0.032
3.175ArgAsp: 3.175 ± 0.062
4.953ArgGlu: 4.953 ± 0.08
2.364ArgPhe: 2.364 ± 0.051
3.895ArgGly: 3.895 ± 0.072
1.086ArgHis: 1.086 ± 0.035
2.877ArgIle: 2.877 ± 0.053
2.905ArgLys: 2.905 ± 0.06
6.133ArgLeu: 6.133 ± 0.106
1.753ArgMet: 1.753 ± 0.044
1.752ArgAsn: 1.752 ± 0.041
2.522ArgPro: 2.522 ± 0.058
2.747ArgGln: 2.747 ± 0.061
4.677ArgArg: 4.677 ± 0.1
2.947ArgSer: 2.947 ± 0.061
3.01ArgThr: 3.01 ± 0.056
3.8ArgVal: 3.8 ± 0.069
0.876ArgTrp: 0.876 ± 0.03
2.314ArgTyr: 2.314 ± 0.049
0.0ArgXaa: 0.0 ± 0.0
Ser
5.384SerAla: 5.384 ± 0.082
0.839SerCys: 0.839 ± 0.029
2.945SerAsp: 2.945 ± 0.064
3.26SerGlu: 3.26 ± 0.067
2.413SerPhe: 2.413 ± 0.053
5.495SerGly: 5.495 ± 0.099
0.996SerHis: 0.996 ± 0.032
3.106SerIle: 3.106 ± 0.057
2.477SerLys: 2.477 ± 0.049
5.496SerLeu: 5.496 ± 0.072
1.506SerMet: 1.506 ± 0.04
1.709SerAsn: 1.709 ± 0.046
2.494SerPro: 2.494 ± 0.049
2.146SerGln: 2.146 ± 0.05
3.178SerArg: 3.178 ± 0.063
3.376SerSer: 3.376 ± 0.077
2.924SerThr: 2.924 ± 0.055
4.143SerVal: 4.143 ± 0.076
0.656SerTrp: 0.656 ± 0.024
2.2SerTyr: 2.2 ± 0.048
0.0SerXaa: 0.0 ± 0.0
Thr
5.91ThrAla: 5.91 ± 0.128
0.702ThrCys: 0.702 ± 0.025
3.042ThrAsp: 3.042 ± 0.064
3.287ThrGlu: 3.287 ± 0.056
2.082ThrPhe: 2.082 ± 0.057
4.96ThrGly: 4.96 ± 0.119
0.951ThrHis: 0.951 ± 0.033
3.2ThrIle: 3.2 ± 0.09
2.0ThrLys: 2.0 ± 0.044
5.604ThrLeu: 5.604 ± 0.087
1.369ThrMet: 1.369 ± 0.039
1.584ThrAsn: 1.584 ± 0.056
3.015ThrPro: 3.015 ± 0.065
1.85ThrGln: 1.85 ± 0.051
2.667ThrArg: 2.667 ± 0.057
2.636ThrSer: 2.636 ± 0.058
2.805ThrThr: 2.805 ± 0.073
5.131ThrVal: 5.131 ± 0.184
0.622ThrTrp: 0.622 ± 0.028
1.776ThrTyr: 1.776 ± 0.067
0.0ThrXaa: 0.0 ± 0.0
Val
5.299ValAla: 5.299 ± 0.08
1.267ValCys: 1.267 ± 0.034
4.118ValAsp: 4.118 ± 0.071
4.587ValGlu: 4.587 ± 0.072
2.817ValPhe: 2.817 ± 0.058
4.582ValGly: 4.582 ± 0.082
1.132ValHis: 1.132 ± 0.03
3.826ValIle: 3.826 ± 0.062
3.419ValLys: 3.419 ± 0.069
7.33ValLeu: 7.33 ± 0.108
1.987ValMet: 1.987 ± 0.046
2.395ValAsn: 2.395 ± 0.045
3.138ValPro: 3.138 ± 0.054
2.218ValGln: 2.218 ± 0.047
3.992ValArg: 3.992 ± 0.073
4.725ValSer: 4.725 ± 0.074
4.674ValThr: 4.674 ± 0.186
4.985ValVal: 4.985 ± 0.09
0.796ValTrp: 0.796 ± 0.03
2.433ValTyr: 2.433 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
1.013TrpAla: 1.013 ± 0.031
0.195TrpCys: 0.195 ± 0.014
0.675TrpAsp: 0.675 ± 0.027
0.882TrpGlu: 0.882 ± 0.031
0.49TrpPhe: 0.49 ± 0.027
0.896TrpGly: 0.896 ± 0.035
0.198TrpHis: 0.198 ± 0.014
0.449TrpIle: 0.449 ± 0.023
0.585TrpLys: 0.585 ± 0.028
1.286TrpLeu: 1.286 ± 0.042
0.337TrpMet: 0.337 ± 0.019
0.475TrpAsn: 0.475 ± 0.021
0.375TrpPro: 0.375 ± 0.017
0.456TrpGln: 0.456 ± 0.02
0.725TrpArg: 0.725 ± 0.028
0.687TrpSer: 0.687 ± 0.028
0.647TrpThr: 0.647 ± 0.026
0.722TrpVal: 0.722 ± 0.027
0.163TrpTrp: 0.163 ± 0.012
0.471TrpTyr: 0.471 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.861TyrAla: 2.861 ± 0.049
0.613TyrCys: 0.613 ± 0.027
2.178TyrAsp: 2.178 ± 0.055
2.288TyrGlu: 2.288 ± 0.053
1.48TyrPhe: 1.48 ± 0.038
2.72TyrGly: 2.72 ± 0.055
0.767TyrHis: 0.767 ± 0.026
2.029TyrIle: 2.029 ± 0.048
1.416TyrLys: 1.416 ± 0.037
3.4TyrLeu: 3.4 ± 0.061
0.791TyrMet: 0.791 ± 0.027
1.281TyrAsn: 1.281 ± 0.037
1.363TyrPro: 1.363 ± 0.048
1.553TyrGln: 1.553 ± 0.042
2.23TyrArg: 2.23 ± 0.053
1.981TyrSer: 1.981 ± 0.054
2.345TyrThr: 2.345 ± 0.077
2.23TyrVal: 2.23 ± 0.056
0.364TyrTrp: 0.364 ± 0.02
1.519TyrTyr: 1.519 ± 0.045
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3472 proteins (1032909 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski