Nocardioides sp. Root122

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4143 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q6VDU1|A0A0Q6VDU1_9ACTN AB hydrolase-1 domain-containing protein OS=Nocardioides sp. Root122 OX=1736431 GN=ASC64_18955 PE=4 SV=1
MM1 pKa = 7.23TLRR4 pKa = 11.84VLSVVLAIAATTGCSSGHH22 pKa = 5.25PQEE25 pKa = 5.72DD26 pKa = 3.78PSSYY30 pKa = 10.83SIVEE34 pKa = 4.34EE35 pKa = 4.16IEE37 pKa = 4.02TGIGTEE43 pKa = 4.18VVVSVEE49 pKa = 4.1DD50 pKa = 3.23SAIAVDD56 pKa = 4.16VFDD59 pKa = 6.01EE60 pKa = 4.77VFDD63 pKa = 5.96DD64 pKa = 3.77NDD66 pKa = 3.72GYY68 pKa = 10.79AAVYY72 pKa = 9.19IFCAGSDD79 pKa = 3.44VRR81 pKa = 11.84QDD83 pKa = 3.96DD84 pKa = 4.43FILYY88 pKa = 9.7GRR90 pKa = 11.84ADD92 pKa = 3.63GDD94 pKa = 3.62DD95 pKa = 3.36RR96 pKa = 11.84NIIIRR101 pKa = 11.84HH102 pKa = 5.65EE103 pKa = 4.34DD104 pKa = 3.17QDD106 pKa = 4.01CC107 pKa = 3.58

Molecular weight: 11.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q6UNZ7|A0A0Q6UNZ7_9ACTN Fe-S cluster assembly ATPase SufC OS=Nocardioides sp. Root122 OX=1736431 GN=sufC PE=3 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.93RR4 pKa = 11.84TYY6 pKa = 10.0QPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 4.97KK15 pKa = 10.04VHH17 pKa = 6.59GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILSSRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.24GRR40 pKa = 11.84KK41 pKa = 8.84SLAVV45 pKa = 3.3

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4143

0

4143

1337500

37

2080

322.8

34.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.07 ± 0.051

0.694 ± 0.01

6.756 ± 0.031

5.755 ± 0.034

2.753 ± 0.026

9.149 ± 0.033

2.289 ± 0.019

3.284 ± 0.025

1.899 ± 0.028

10.227 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.899 ± 0.015

1.678 ± 0.017

5.525 ± 0.031

2.703 ± 0.022

7.651 ± 0.041

5.296 ± 0.025

6.218 ± 0.033

9.7 ± 0.037

1.592 ± 0.015

1.859 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski