Achimota virus 1
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K7X4M6|K7X4M6_9MONO Fusion glycoprotein F0 OS=Achimota virus 1 OX=1261100 GN=F PE=3 SV=1
MM1 pKa = 7.43 SSVFKK6 pKa = 10.91 AFEE9 pKa = 4.08 QFTLEE14 pKa = 3.48 QDD16 pKa = 3.57 QYY18 pKa = 11.86 VGGNNLEE25 pKa = 4.56 LPPEE29 pKa = 4.29 TLQTTIKK36 pKa = 10.68 VFILNTQNPQTRR48 pKa = 11.84 YY49 pKa = 10.35 QMLCFCLRR57 pKa = 11.84 IIASNSARR65 pKa = 11.84 TAQKK69 pKa = 9.4 YY70 pKa = 9.41 GAMLTVLSLPTAMMHH85 pKa = 4.62 NHH87 pKa = 5.21 MRR89 pKa = 11.84 IADD92 pKa = 4.44 RR93 pKa = 11.84 SPDD96 pKa = 3.79 SIIEE100 pKa = 4.32 RR101 pKa = 11.84 IEE103 pKa = 3.76 IEE105 pKa = 4.03 GFEE108 pKa = 4.28 PGSYY112 pKa = 9.93 RR113 pKa = 11.84 LRR115 pKa = 11.84 PNARR119 pKa = 11.84 TPMTQGEE126 pKa = 4.68 IIAMEE131 pKa = 4.32 NMADD135 pKa = 4.26 DD136 pKa = 4.89 LPDD139 pKa = 4.01 TIVNQTPFVNTATEE153 pKa = 4.93 DD154 pKa = 4.0 EE155 pKa = 4.76 ICDD158 pKa = 3.7 EE159 pKa = 4.21 MEE161 pKa = 4.03 KK162 pKa = 10.66 FLNAVYY168 pKa = 10.47 SVLIQVWVMVCKK180 pKa = 10.67 CMTAFDD186 pKa = 4.24 QPTGSDD192 pKa = 2.97 ARR194 pKa = 11.84 RR195 pKa = 11.84 LAKK198 pKa = 10.15 YY199 pKa = 7.91 QQQGRR204 pKa = 11.84 MDD206 pKa = 3.5 QKK208 pKa = 10.9 YY209 pKa = 8.56 VLQGEE214 pKa = 4.53 VRR216 pKa = 11.84 RR217 pKa = 11.84 LIQLCIRR224 pKa = 11.84 EE225 pKa = 4.45 SLTIRR230 pKa = 11.84 QFLVNEE236 pKa = 4.03 MQTARR241 pKa = 11.84 RR242 pKa = 11.84 QGPITGKK249 pKa = 9.65 YY250 pKa = 7.68 YY251 pKa = 11.56 AMVGDD256 pKa = 3.68 VARR259 pKa = 11.84 YY260 pKa = 9.28 IEE262 pKa = 4.23 NAGMGAFFMTVKK274 pKa = 10.46 FALGNRR280 pKa = 11.84 WPPLALAAFSGEE292 pKa = 4.01 LVKK295 pKa = 10.88 IKK297 pKa = 10.64 GLMMLYY303 pKa = 10.23 RR304 pKa = 11.84 KK305 pKa = 10.32 LGDD308 pKa = 3.31 RR309 pKa = 11.84 AKK311 pKa = 11.1 YY312 pKa = 8.51 MALLEE317 pKa = 4.24 MSEE320 pKa = 4.3 MMDD323 pKa = 3.75 FAPANYY329 pKa = 9.58 QLLYY333 pKa = 10.1 SYY335 pKa = 11.65 AMGIGSVQDD344 pKa = 3.56 PQMRR348 pKa = 11.84 GYY350 pKa = 11.07 NFARR354 pKa = 11.84 PFLNPAFFQLGMEE367 pKa = 4.37 TANRR371 pKa = 11.84 QQGSVDD377 pKa = 3.22 RR378 pKa = 11.84 EE379 pKa = 3.97 MAEE382 pKa = 4.08 EE383 pKa = 4.62 LGLTDD388 pKa = 3.28 TEE390 pKa = 4.15 RR391 pKa = 11.84 RR392 pKa = 11.84 EE393 pKa = 3.76 MAAQVTRR400 pKa = 11.84 LTTGRR405 pKa = 11.84 GAGDD409 pKa = 3.22 QQDD412 pKa = 3.17 IVNIFGARR420 pKa = 11.84 ARR422 pKa = 11.84 NGRR425 pKa = 11.84 HH426 pKa = 5.43 GAQEE430 pKa = 3.68 RR431 pKa = 11.84 QFRR434 pKa = 11.84 VIEE437 pKa = 4.33 GDD439 pKa = 3.78 DD440 pKa = 3.52 VDD442 pKa = 5.49 DD443 pKa = 5.94 EE444 pKa = 4.49 EE445 pKa = 7.19 GEE447 pKa = 6.18 DD448 pKa = 5.04 DD449 pKa = 5.7 DD450 pKa = 5.78 QDD452 pKa = 4.07 NDD454 pKa = 3.74 DD455 pKa = 5.14 EE456 pKa = 4.35 EE457 pKa = 4.71 AQRR460 pKa = 11.84 QQYY463 pKa = 8.49 IANEE467 pKa = 3.9 QARR470 pKa = 11.84 WQARR474 pKa = 11.84 LAEE477 pKa = 4.19 IEE479 pKa = 4.01 EE480 pKa = 4.15 RR481 pKa = 11.84 QAAEE485 pKa = 3.77 RR486 pKa = 11.84 GRR488 pKa = 11.84 NRR490 pKa = 11.84 QGRR493 pKa = 11.84 QQDD496 pKa = 3.33 QARR499 pKa = 11.84 DD500 pKa = 3.57 IQRR503 pKa = 11.84 ATAQVHH509 pKa = 5.03 QEE511 pKa = 3.83 PAAQDD516 pKa = 4.44 LIADD520 pKa = 5.31 LIEE523 pKa = 4.04
Molecular weight: 59.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.959
IPC2_protein 4.978
IPC_protein 4.914
Toseland 4.774
ProMoST 4.978
Dawson 4.851
Bjellqvist 5.016
Wikipedia 4.711
Rodwell 4.762
Grimsley 4.685
Solomon 4.851
Lehninger 4.813
Nozaki 4.965
DTASelect 5.105
Thurlkill 4.762
EMBOSS 4.736
Sillero 5.029
Patrickios 4.164
IPC_peptide 4.863
IPC2_peptide 5.029
IPC2.peptide.svr19 4.995
Protein with the highest isoelectric point:
>tr|K7Y0L1|K7Y0L1_9MONO Phosphoprotein OS=Achimota virus 1 OX=1261100 GN=P PE=3 SV=1
MM1 pKa = 7.7 AHH3 pKa = 6.39 RR4 pKa = 11.84 QASVSLKK11 pKa = 10.01 VDD13 pKa = 3.25 PVSEE17 pKa = 4.31 KK18 pKa = 10.2 NHH20 pKa = 5.94 LRR22 pKa = 11.84 PFPIVQVTPDD32 pKa = 3.28 SGNGRR37 pKa = 11.84 GRR39 pKa = 11.84 LMKK42 pKa = 10.21 QIRR45 pKa = 11.84 IKK47 pKa = 10.92 DD48 pKa = 3.67 LTPAGSTEE56 pKa = 3.9 PPITFVNTYY65 pKa = 10.69 GFVKK69 pKa = 10.34 PLRR72 pKa = 11.84 TRR74 pKa = 11.84 SEE76 pKa = 4.2 FFSEE80 pKa = 4.18 FHH82 pKa = 6.89 RR83 pKa = 11.84 PDD85 pKa = 3.24 SSPSITACTLPFGAGPNIDD104 pKa = 3.26 HH105 pKa = 7.55 PMRR108 pKa = 11.84 LLEE111 pKa = 4.83 EE112 pKa = 4.05 IEE114 pKa = 4.08 KK115 pKa = 10.61 CHH117 pKa = 6.94 IVMRR121 pKa = 11.84 KK122 pKa = 8.62 SASLRR127 pKa = 11.84 EE128 pKa = 4.08 EE129 pKa = 3.92 VVYY132 pKa = 9.17 TIQKK136 pKa = 9.67 LPPILAYY143 pKa = 10.17 HH144 pKa = 6.26 QIASQKK150 pKa = 9.57 LICVPSEE157 pKa = 4.37 KK158 pKa = 10.36 YY159 pKa = 10.6 LKK161 pKa = 10.56 APSKK165 pKa = 8.81 MQSGVDD171 pKa = 3.36 YY172 pKa = 8.25 TYY174 pKa = 10.7 CISFLSIVYY183 pKa = 8.82 CPPSLKK189 pKa = 10.27 FRR191 pKa = 11.84 VLRR194 pKa = 11.84 PLQMMRR200 pKa = 11.84 SSTMRR205 pKa = 11.84 SIQIEE210 pKa = 4.15 VLLEE214 pKa = 3.91 VDD216 pKa = 5.01 CDD218 pKa = 4.06 DD219 pKa = 4.57 SSPATRR225 pKa = 11.84 FLIRR229 pKa = 11.84 DD230 pKa = 3.37 EE231 pKa = 4.51 ATQKK235 pKa = 9.32 WRR237 pKa = 11.84 ASVWFHH243 pKa = 6.44 LCNIIKK249 pKa = 9.87 SHH251 pKa = 6.21 QGGDD255 pKa = 3.53 KK256 pKa = 11.17 YY257 pKa = 11.28 DD258 pKa = 3.3 DD259 pKa = 4.24 HH260 pKa = 8.21 YY261 pKa = 11.97 FNDD264 pKa = 3.79 KK265 pKa = 9.32 CRR267 pKa = 11.84 KK268 pKa = 7.7 MDD270 pKa = 4.01 LEE272 pKa = 4.35 VGIIDD277 pKa = 3.23 MWGPTFLVKK286 pKa = 10.56 AHH288 pKa = 5.6 GRR290 pKa = 11.84 VPKK293 pKa = 9.93 SAKK296 pKa = 10.27 VYY298 pKa = 10.02 FSQKK302 pKa = 8.94 NWACHH307 pKa = 5.65 PLVDD311 pKa = 5.19 AAPALSKK318 pKa = 10.69 ILWSVGASIVQVNAILQPSDD338 pKa = 4.08 LNQLAQFSDD347 pKa = 3.9 VIYY350 pKa = 9.69 PKK352 pKa = 10.87 VKK354 pKa = 10.1 INRR357 pKa = 11.84 ALAGSPPSKK366 pKa = 9.84 WNPVKK371 pKa = 10.64 KK372 pKa = 10.41 AVLGG376 pKa = 3.89
Molecular weight: 42.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.858
IPC2_protein 8.726
IPC_protein 8.609
Toseland 9.37
ProMoST 9.151
Dawson 9.648
Bjellqvist 9.443
Wikipedia 9.794
Rodwell 9.97
Grimsley 9.692
Solomon 9.692
Lehninger 9.648
Nozaki 9.633
DTASelect 9.355
Thurlkill 9.516
EMBOSS 9.823
Sillero 9.648
Patrickios 5.08
IPC_peptide 9.677
IPC2_peptide 8.17
IPC2.peptide.svr19 7.841
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
2
8
5106
166
2271
638.3
71.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.091 ± 0.77
1.88 ± 0.26
5.112 ± 0.399
5.464 ± 0.519
3.662 ± 0.168
5.014 ± 0.493
1.861 ± 0.285
7.462 ± 0.555
5.288 ± 0.565
10.145 ± 1.373
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.742 ± 0.409
5.112 ± 0.522
5.484 ± 0.792
4.955 ± 0.897
5.092 ± 0.505
8.48 ± 0.948
5.895 ± 0.438
6.013 ± 0.385
1.018 ± 0.186
3.231 ± 0.463
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here