Beihai tombus-like virus 7
Average proteome isoelectric point is 7.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFQ2|A0A1L3KFQ2_9VIRU C2H2-type domain-containing protein OS=Beihai tombus-like virus 7 OX=1922728 PE=4 SV=1
MM1 pKa = 7.66 HH2 pKa = 7.68 SITCPHH8 pKa = 6.11 CQKK11 pKa = 10.36 VFEE14 pKa = 4.65 FPSKK18 pKa = 9.95 RR19 pKa = 11.84 KK20 pKa = 9.94 ASIRR24 pKa = 11.84 EE25 pKa = 3.93 HH26 pKa = 6.76 LLAEE30 pKa = 4.69 HH31 pKa = 6.06 PHH33 pKa = 4.6 IHH35 pKa = 6.29 LRR37 pKa = 11.84 CEE39 pKa = 3.74 QRR41 pKa = 11.84 GAAEE45 pKa = 4.15 TNRR48 pKa = 11.84 VWFRR52 pKa = 11.84 CVLCDD57 pKa = 3.31 EE58 pKa = 5.0 TYY60 pKa = 11.51 NDD62 pKa = 3.45 LSVYY66 pKa = 9.52 EE67 pKa = 3.98 LHH69 pKa = 7.15 LVTHH73 pKa = 6.72 HH74 pKa = 6.23 EE75 pKa = 4.08 AEE77 pKa = 4.65 QMDD80 pKa = 4.55 FDD82 pKa = 5.5 SNAVPLDD89 pKa = 3.93 TMDD92 pKa = 3.88 KK93 pKa = 8.93 TCKK96 pKa = 10.18 LHH98 pKa = 6.04 MLRR101 pKa = 11.84 QRR103 pKa = 11.84 RR104 pKa = 11.84 TCKK107 pKa = 10.26 RR108 pKa = 11.84 EE109 pKa = 3.42 PDD111 pKa = 3.27 LFGALEE117 pKa = 4.06 ISHH120 pKa = 6.0 VV121 pKa = 3.6
Molecular weight: 14.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.206
IPC2_protein 6.122
IPC_protein 6.249
Toseland 6.547
ProMoST 6.678
Dawson 6.62
Bjellqvist 6.576
Wikipedia 6.649
Rodwell 6.62
Grimsley 6.678
Solomon 6.634
Lehninger 6.634
Nozaki 6.912
DTASelect 7.073
Thurlkill 7.102
EMBOSS 7.088
Sillero 7.029
Patrickios 4.062
IPC_peptide 6.649
IPC2_peptide 6.985
IPC2.peptide.svr19 6.843
Protein with the highest isoelectric point:
>tr|A0A1L3KFN4|A0A1L3KFN4_9VIRU RNA-directed RNA polymerase OS=Beihai tombus-like virus 7 OX=1922728 PE=4 SV=1
MM1 pKa = 7.08 QLASARR7 pKa = 11.84 QMSSHH12 pKa = 6.16 KK13 pKa = 10.2 VVPSADD19 pKa = 3.83 DD20 pKa = 4.5 GYY22 pKa = 11.88 GWDD25 pKa = 4.63 CPTLQLASQNSGFRR39 pKa = 11.84 QIGPGWDD46 pKa = 2.96 TMTSQRR52 pKa = 11.84 NRR54 pKa = 11.84 GSARR58 pKa = 11.84 GSQAYY63 pKa = 8.86 VVHH66 pKa = 6.93 LYY68 pKa = 10.96 AHH70 pKa = 7.45 LSTNQSGWVARR81 pKa = 11.84 SYY83 pKa = 11.9 AFLPTSAKK91 pKa = 9.6 MISGPSRR98 pKa = 11.84 FILLPLMDD106 pKa = 3.82 YY107 pKa = 7.57 TARR110 pKa = 11.84 SPIAGAHH117 pKa = 5.66 AMNFLLYY124 pKa = 10.54 AIEE127 pKa = 4.0
Molecular weight: 13.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.823
IPC2_protein 9.326
IPC_protein 9.75
Toseland 9.326
ProMoST 9.37
Dawson 9.706
Bjellqvist 9.575
Wikipedia 9.999
Rodwell 9.736
Grimsley 9.853
Solomon 9.809
Lehninger 9.75
Nozaki 9.414
DTASelect 9.545
Thurlkill 9.502
EMBOSS 9.78
Sillero 9.663
Patrickios 4.329
IPC_peptide 9.794
IPC2_peptide 8.682
IPC2.peptide.svr19 8.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1567
121
1036
391.8
44.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.658 ± 0.979
2.616 ± 0.599
4.786 ± 0.842
5.169 ± 0.86
2.999 ± 0.308
6.126 ± 1.057
3.063 ± 1.354
4.659 ± 0.839
3.765 ± 0.377
9.892 ± 1.567
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.425 ± 0.909
3.765 ± 0.703
6.509 ± 0.876
4.212 ± 0.423
8.168 ± 1.253
7.084 ± 0.933
5.807 ± 0.457
6.445 ± 0.618
1.659 ± 0.26
3.127 ± 0.701
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here