Citrobacter phage CF1 ERZ-2017
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 306 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H4YFQ0|A0A2H4YFQ0_9CAUD Uncharacterized protein OS=Citrobacter phage CF1 ERZ-2017 OX=2267236 GN=Cf1_00118 PE=4 SV=1
MM1 pKa = 6.44 TTAEE5 pKa = 4.57 LKK7 pKa = 10.83 VEE9 pKa = 4.0 LEE11 pKa = 4.05 KK12 pKa = 11.01 LVEE15 pKa = 4.01 LSEE18 pKa = 4.49 YY19 pKa = 11.36 AMQSYY24 pKa = 10.64 NFGTDD29 pKa = 2.79 EE30 pKa = 4.39 EE31 pKa = 5.25 YY32 pKa = 10.58 INDD35 pKa = 3.54 TEE37 pKa = 5.37 ARR39 pKa = 11.84 DD40 pKa = 3.83 NQIGYY45 pKa = 9.59 ILDD48 pKa = 3.43 IFGDD52 pKa = 3.8 MM53 pKa = 4.71
Molecular weight: 6.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.798
IPC2_protein 3.808
IPC_protein 3.668
Toseland 3.503
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.821
Wikipedia 3.579
Rodwell 3.516
Grimsley 3.427
Solomon 3.617
Lehninger 3.567
Nozaki 3.795
DTASelect 3.897
Thurlkill 3.567
EMBOSS 3.592
Sillero 3.783
Patrickios 1.825
IPC_peptide 3.617
IPC2_peptide 3.757
IPC2.peptide.svr19 3.75
Protein with the highest isoelectric point:
>tr|A0A2H4YGP5|A0A2H4YGP5_9CAUD Uncharacterized protein OS=Citrobacter phage CF1 ERZ-2017 OX=2267236 GN=Cf1_00311 PE=4 SV=1
MM1 pKa = 7.48 SKK3 pKa = 10.61 AKK5 pKa = 10.34 KK6 pKa = 9.43 AVKK9 pKa = 9.76 SVKK12 pKa = 10.09 EE13 pKa = 3.83 VVGTSKK19 pKa = 10.63 RR20 pKa = 11.84 AGYY23 pKa = 9.64 KK24 pKa = 9.6 RR25 pKa = 11.84 STNARR30 pKa = 11.84 IDD32 pKa = 3.49 QLGDD36 pKa = 3.28 QLASRR41 pKa = 11.84 ARR43 pKa = 11.84 KK44 pKa = 9.36 VLAHH48 pKa = 6.98 DD49 pKa = 3.84 AAFGNPRR56 pKa = 11.84 KK57 pKa = 9.89 KK58 pKa = 10.66 AA59 pKa = 3.36
Molecular weight: 6.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.249
IPC2_protein 10.043
IPC_protein 10.906
Toseland 11.359
ProMoST 11.286
Dawson 11.389
Bjellqvist 11.14
Wikipedia 11.652
Rodwell 11.725
Grimsley 11.418
Solomon 11.637
Lehninger 11.579
Nozaki 11.33
DTASelect 11.14
Thurlkill 11.33
EMBOSS 11.784
Sillero 11.345
Patrickios 11.462
IPC_peptide 11.637
IPC2_peptide 10.087
IPC2.peptide.svr19 8.549
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
306
0
306
53823
31
1265
175.9
19.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.776 ± 0.163
1.13 ± 0.078
6.425 ± 0.112
7.292 ± 0.169
4.37 ± 0.094
6.204 ± 0.156
1.86 ± 0.085
7.192 ± 0.125
7.391 ± 0.175
7.541 ± 0.124
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.023 ± 0.081
5.336 ± 0.108
3.463 ± 0.105
3.413 ± 0.09
4.584 ± 0.083
6.293 ± 0.137
5.656 ± 0.184
6.475 ± 0.11
1.486 ± 0.063
4.091 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here