Ornithinimicrobium sp. H23M54
Average proteome isoelectric point is 5.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3622 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A516GEQ0|A0A516GEQ0_9MICO Glycosyltransferase family 2 protein OS=Ornithinimicrobium sp. H23M54 OX=2594265 GN=FNH13_18165 PE=4 SV=1
MM1 pKa = 7.5 AVHH4 pKa = 6.16 GHH6 pKa = 5.92 RR7 pKa = 11.84 ARR9 pKa = 11.84 RR10 pKa = 11.84 WLAAVAGASLVASGFGLAGASAQPPQPMAEE40 pKa = 3.86 VGATDD45 pKa = 4.83 KK46 pKa = 10.92 IDD48 pKa = 3.8 EE49 pKa = 4.35 EE50 pKa = 4.67 VSAQIEE56 pKa = 4.4 EE57 pKa = 4.23 KK58 pKa = 10.64 GAADD62 pKa = 3.71 FWVRR66 pKa = 11.84 FDD68 pKa = 4.24 GRR70 pKa = 11.84 PDD72 pKa = 3.1 MSQFSSISDD81 pKa = 3.05 WDD83 pKa = 3.51 ARR85 pKa = 11.84 GQAVYY90 pKa = 10.66 DD91 pKa = 4.13 ALTEE95 pKa = 4.16 SADD98 pKa = 4.32 ASQQDD103 pKa = 3.16 IRR105 pKa = 11.84 EE106 pKa = 4.04 QLDD109 pKa = 3.47 ADD111 pKa = 3.95 GVDD114 pKa = 4.4 YY115 pKa = 11.03 QAFWATNAIRR125 pKa = 11.84 ITSGDD130 pKa = 3.3 QSLVSTLAADD140 pKa = 3.62 SGVEE144 pKa = 4.32 GIYY147 pKa = 8.5 PTTTYY152 pKa = 8.86 EE153 pKa = 4.0 APEE156 pKa = 3.97 LVEE159 pKa = 4.92 ADD161 pKa = 4.03 RR162 pKa = 11.84 EE163 pKa = 4.23 MSPSAVEE170 pKa = 3.77 WGVADD175 pKa = 4.75 INADD179 pKa = 4.1 DD180 pKa = 3.43 VWSTYY185 pKa = 9.22 GVHH188 pKa = 6.84 GEE190 pKa = 4.35 GIVVGSIDD198 pKa = 4.3 TGVQWDD204 pKa = 4.19 HH205 pKa = 6.93 PALVDD210 pKa = 4.72 AYY212 pKa = 10.43 RR213 pKa = 11.84 GNNGDD218 pKa = 3.42 GTFTHH223 pKa = 7.61 DD224 pKa = 3.92 YY225 pKa = 9.95 NWYY228 pKa = 9.31 DD229 pKa = 3.43 AAGTSPEE236 pKa = 4.22 VPADD240 pKa = 3.81 GNGHH244 pKa = 4.99 GTHH247 pKa = 5.45 VTGTMVGDD255 pKa = 4.21 DD256 pKa = 3.53 GGEE259 pKa = 4.11 NQVGVAPGATWISANGCCPSDD280 pKa = 3.48 QALIDD285 pKa = 3.6 SGQWMLAPTKK295 pKa = 10.68 LDD297 pKa = 3.24 GTSPDD302 pKa = 3.36 TTKK305 pKa = 10.66 RR306 pKa = 11.84 PHH308 pKa = 7.13 IINNSWGTTLPSNDD322 pKa = 3.57 PFMEE326 pKa = 4.26 DD327 pKa = 4.99 VIEE330 pKa = 4.34 AWDD333 pKa = 3.46 AAGIFSMWANGNSGPACNTSGAPGSRR359 pKa = 11.84 IITYY363 pKa = 10.34 SVGNYY368 pKa = 9.23 NSSHH372 pKa = 6.8 AISNTSGKK380 pKa = 10.58 GAGQDD385 pKa = 4.11 GEE387 pKa = 4.4 IKK389 pKa = 10.53 PNIAAPGSAIRR400 pKa = 11.84 SSVPGNGYY408 pKa = 10.96 ANYY411 pKa = 9.79 SGTSMASPHH420 pKa = 6.29 AAGAVALLWSAAPSLVGDD438 pKa = 3.92 IEE440 pKa = 4.42 GTRR443 pKa = 11.84 ALLDD447 pKa = 3.21 GTAIDD452 pKa = 4.75 TEE454 pKa = 4.17 NLTCGGTADD463 pKa = 4.16 DD464 pKa = 4.36 NNVFGEE470 pKa = 4.34 GRR472 pKa = 11.84 LDD474 pKa = 3.45 ALALIEE480 pKa = 4.46 AAPTGDD486 pKa = 3.27 TGEE489 pKa = 4.41 LNGTVTDD496 pKa = 4.63 ADD498 pKa = 3.7 TGDD501 pKa = 4.69 AISGANVVIVGEE513 pKa = 4.25 AEE515 pKa = 3.98 RR516 pKa = 11.84 TTVTDD521 pKa = 3.55 AEE523 pKa = 4.59 GTYY526 pKa = 10.85 SSLLVAGDD534 pKa = 3.63 YY535 pKa = 11.21 DD536 pKa = 3.97 VTVSKK541 pKa = 10.55 FGYY544 pKa = 8.34 GTEE547 pKa = 4.39 TEE549 pKa = 4.68 SVTVPAGGTATLDD562 pKa = 3.49 FDD564 pKa = 5.97 LEE566 pKa = 4.42 AQEE569 pKa = 4.35 STAVSGVVTDD579 pKa = 4.68 GSGHH583 pKa = 6.18 GWPVYY588 pKa = 10.87 AKK590 pKa = 10.6 VSVQGTDD597 pKa = 2.5 VSTYY601 pKa = 9.08 TDD603 pKa = 3.97 PATGEE608 pKa = 4.08 YY609 pKa = 10.02 TLALPIGADD618 pKa = 3.27 YY619 pKa = 10.12 TLEE622 pKa = 4.26 VEE624 pKa = 4.34 AQYY627 pKa = 10.76 PGYY630 pKa = 8.62 EE631 pKa = 4.01 TATVVVSPQGAPLTGSRR648 pKa = 11.84 EE649 pKa = 3.98 SAKK652 pKa = 10.18 RR653 pKa = 11.84 VPQVVQDD660 pKa = 3.34 IQVIVDD666 pKa = 3.88 ADD668 pKa = 3.77 SCVAPGYY675 pKa = 9.31 AHH677 pKa = 6.71 SSEE680 pKa = 4.22 GVVEE684 pKa = 4.59 SFDD687 pKa = 3.55 GTEE690 pKa = 4.14 TPEE693 pKa = 3.86 GWTVTDD699 pKa = 4.28 DD700 pKa = 3.71 AGTGQVWLFDD710 pKa = 4.11 DD711 pKa = 3.7 SRR713 pKa = 11.84 NRR715 pKa = 11.84 GNLTGGEE722 pKa = 3.84 GGYY725 pKa = 10.83 AIVDD729 pKa = 3.5 SDD731 pKa = 4.02 NYY733 pKa = 11.11 GSGGVQDD740 pKa = 4.2 TSLVTPVVDD749 pKa = 4.15 MSALAEE755 pKa = 3.83 PVIGFKK761 pKa = 10.26 HH762 pKa = 6.99 DD763 pKa = 3.55 YY764 pKa = 10.4 NAFLGTGEE772 pKa = 4.16 SGEE775 pKa = 4.15 VDD777 pKa = 3.41 VSIDD781 pKa = 4.25 GGEE784 pKa = 3.99 TWEE787 pKa = 4.23 TLATYY792 pKa = 7.64 TTDD795 pKa = 2.88 SRR797 pKa = 11.84 GEE799 pKa = 4.14 VVLPMPTAAGEE810 pKa = 4.02 SDD812 pKa = 3.75 VQVRR816 pKa = 11.84 FHH818 pKa = 6.96 YY819 pKa = 10.24 SASWDD824 pKa = 3.59 YY825 pKa = 8.9 WWQVDD830 pKa = 4.49 DD831 pKa = 4.34 VFVGNRR837 pKa = 11.84 TCEE840 pKa = 3.63 PSIAGGYY847 pKa = 8.72 VVGTVASAEE856 pKa = 4.16 TGEE859 pKa = 4.68 GIVGASVTSVEE870 pKa = 4.34 APEE873 pKa = 4.42 EE874 pKa = 4.22 SATTVATPDD883 pKa = 5.03 DD884 pKa = 4.37 EE885 pKa = 5.26 NLDD888 pKa = 4.13 DD889 pKa = 4.03 GFYY892 pKa = 10.19 WIFSTLTGAHH902 pKa = 6.65 DD903 pKa = 4.5 FEE905 pKa = 4.74 ATARR909 pKa = 11.84 GYY911 pKa = 11.12 EE912 pKa = 4.27 SVTQSVDD919 pKa = 3.21 VAEE922 pKa = 5.14 DD923 pKa = 3.55 DD924 pKa = 4.09 AVRR927 pKa = 11.84 ADD929 pKa = 3.6 FTLGSGLVDD938 pKa = 3.49 VSPDD942 pKa = 3.43 SITTYY947 pKa = 10.5 VQLGDD952 pKa = 3.86 SVTDD956 pKa = 3.78 KK957 pKa = 10.76 LTISNTGTGAAEE969 pKa = 4.08 VTLSEE974 pKa = 4.23 LAGDD978 pKa = 4.11 FEE980 pKa = 4.9 ILRR983 pKa = 11.84 ADD985 pKa = 3.57 GTKK988 pKa = 10.48 EE989 pKa = 4.28 LMSEE993 pKa = 3.81 MHH995 pKa = 5.86 KK996 pKa = 9.77 TEE998 pKa = 4.23 GAPLQEE1004 pKa = 4.53 IEE1006 pKa = 4.83 AEE1008 pKa = 4.3 VSFAQHH1014 pKa = 6.31 ASGLAAQAEE1023 pKa = 4.29 PAARR1027 pKa = 11.84 GVAEE1031 pKa = 4.94 APWTDD1036 pKa = 3.65 LPQLGSVNMDD1046 pKa = 2.61 GRR1048 pKa = 11.84 AVNLDD1053 pKa = 3.49 GTWYY1057 pKa = 10.68 LITGGSGSASYY1068 pKa = 11.59 ADD1070 pKa = 3.68 VLRR1073 pKa = 11.84 YY1074 pKa = 9.63 DD1075 pKa = 4.34 AEE1077 pKa = 4.45 DD1078 pKa = 3.7 MSWTAVAPLPSARR1091 pKa = 11.84 NAVTAGVVNGQIVVSGGWVAAGTTAEE1117 pKa = 4.15 TFVYY1121 pKa = 10.68 DD1122 pKa = 4.43 EE1123 pKa = 5.42 GADD1126 pKa = 3.32 SWTAVADD1133 pKa = 3.72 NPVAVSAAGQAVADD1147 pKa = 4.15 GKK1149 pKa = 10.74 LYY1151 pKa = 11.03 SVGGCSSADD1160 pKa = 3.53 CTPMSNAVTAYY1171 pKa = 10.61 DD1172 pKa = 4.24 PGTDD1176 pKa = 3.05 TWEE1179 pKa = 4.04 TLANYY1184 pKa = 7.54 PAPVAFASCGGINGEE1199 pKa = 4.61 VYY1201 pKa = 9.44 CTGGNGGAAGTAASYY1216 pKa = 11.22 VYY1218 pKa = 10.53 TPDD1221 pKa = 3.21 TDD1223 pKa = 3.04 TWTAIADD1230 pKa = 3.79 APIDD1234 pKa = 3.62 TWASQYY1240 pKa = 11.21 AAANGMLIVNGGVQGGTITNATFAYY1265 pKa = 10.29 DD1266 pKa = 3.62 PSTDD1270 pKa = 3.12 EE1271 pKa = 5.12 WVDD1274 pKa = 3.84 LPNSNTAVYY1283 pKa = 9.86 RR1284 pKa = 11.84 GAAACGFGKK1293 pKa = 10.67 FGGSSGNFNATANTEE1308 pKa = 4.12 YY1309 pKa = 11.08 LPGFDD1314 pKa = 4.45 DD1315 pKa = 5.08 CGAAGADD1322 pKa = 3.99 VEE1324 pKa = 4.49 WLSLSATEE1332 pKa = 4.03 LTIPAGEE1339 pKa = 4.38 SVTVDD1344 pKa = 2.97 VTTDD1348 pKa = 3.22 GNVAQPGVYY1357 pKa = 8.65 TAGIKK1362 pKa = 10.33 VSANTPQSFDD1372 pKa = 4.45 PIPVTMHH1379 pKa = 5.99 VSPPRR1384 pKa = 11.84 PWGKK1388 pKa = 10.83 LMGNVSGVDD1397 pKa = 3.78 CDD1399 pKa = 3.94 TVTAPIAGATVDD1411 pKa = 3.43 ITPTRR1416 pKa = 11.84 TDD1418 pKa = 2.8 HH1419 pKa = 6.63 PGWVLSTDD1427 pKa = 3.23 ADD1429 pKa = 3.3 GDD1431 pKa = 3.93 YY1432 pKa = 10.28 ATWVNTQHH1440 pKa = 5.9 TRR1442 pKa = 11.84 PGGVQIIAAKK1452 pKa = 10.48 DD1453 pKa = 3.2 GWRR1456 pKa = 11.84 PKK1458 pKa = 10.6 AATADD1463 pKa = 3.79 PARR1466 pKa = 11.84 GVVRR1470 pKa = 11.84 TVNFTLAKK1478 pKa = 9.76 IGCC1481 pKa = 4.1
Molecular weight: 152.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.795
IPC_protein 3.834
Toseland 3.617
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.745
Rodwell 3.656
Grimsley 3.516
Solomon 3.821
Lehninger 3.783
Nozaki 3.923
DTASelect 4.177
Thurlkill 3.656
EMBOSS 3.757
Sillero 3.961
Patrickios 1.265
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.833
Protein with the highest isoelectric point:
>tr|A0A516G6M8|A0A516G6M8_9MICO Formyltetrahydrofolate deformylase OS=Ornithinimicrobium sp. H23M54 OX=2594265 GN=purU PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SRR15 pKa = 11.84 VHH17 pKa = 6.33 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 SILASRR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.66 GRR40 pKa = 11.84 TKK42 pKa = 10.86 LSAA45 pKa = 3.61
Molecular weight: 5.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.676
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.398
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.243
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3622
0
3622
1205281
32
3158
332.8
35.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.637 ± 0.048
0.667 ± 0.011
6.3 ± 0.039
6.06 ± 0.038
2.653 ± 0.024
9.27 ± 0.039
2.299 ± 0.021
3.707 ± 0.031
1.662 ± 0.026
10.395 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.887 ± 0.018
1.741 ± 0.021
5.744 ± 0.031
3.3 ± 0.019
7.277 ± 0.049
5.458 ± 0.033
6.414 ± 0.038
9.06 ± 0.036
1.589 ± 0.015
1.88 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here