Streptomyces sp. SID625

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9651 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I5C8E8|A0A6I5C8E8_9ACTN NADPH:quinone reductase (Fragment) OS=Streptomyces sp. SID625 OX=2690321 GN=GTY54_16420 PE=4 SV=1
MM1 pKa = 7.65SVQQQTADD9 pKa = 3.3GDD11 pKa = 3.96EE12 pKa = 4.25EE13 pKa = 5.18LEE15 pKa = 4.13VWIDD19 pKa = 3.43QDD21 pKa = 3.99LCTGDD26 pKa = 5.01GICAQYY32 pKa = 10.9APEE35 pKa = 4.23VFEE38 pKa = 5.9LDD40 pKa = 3.05IDD42 pKa = 3.65GLAYY46 pKa = 10.7VKK48 pKa = 11.02GPGDD52 pKa = 4.09EE53 pKa = 4.71LLQAQGAATPVPLPLLTDD71 pKa = 3.69VVDD74 pKa = 4.29SARR77 pKa = 11.84EE78 pKa = 3.94CPGEE82 pKa = 4.38CIHH85 pKa = 6.36VRR87 pKa = 11.84RR88 pKa = 11.84VLDD91 pKa = 3.47RR92 pKa = 11.84VEE94 pKa = 4.7VYY96 pKa = 10.88GPDD99 pKa = 3.22AGG101 pKa = 4.06

Molecular weight: 10.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I5CQN5|A0A6I5CQN5_9ACTN ATP-binding cassette domain-containing protein (Fragment) OS=Streptomyces sp. SID625 OX=2690321 GN=GTY54_45250 PE=4 SV=1
MM1 pKa = 7.62VSGLLVRR8 pKa = 11.84VGPLSRR14 pKa = 11.84SRR16 pKa = 11.84LLSRR20 pKa = 11.84PRR22 pKa = 11.84LLSRR26 pKa = 11.84SRR28 pKa = 11.84LLRR31 pKa = 11.84MLPAPWRR38 pKa = 11.84LFSWPHH44 pKa = 4.72GRR46 pKa = 4.45

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9651

0

9651

2221653

19

2189

230.2

24.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.772 ± 0.042

0.831 ± 0.009

6.037 ± 0.022

5.59 ± 0.029

2.698 ± 0.015

9.537 ± 0.028

2.419 ± 0.015

2.926 ± 0.018

2.067 ± 0.024

10.433 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.658 ± 0.011

1.665 ± 0.015

6.063 ± 0.02

2.756 ± 0.018

8.332 ± 0.03

4.912 ± 0.019

6.183 ± 0.024

8.503 ± 0.026

1.504 ± 0.012

2.116 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski